Araştırma Makalesi
BibTex RIS Kaynak Göster

KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ

Yıl 2013, Cilt: 17 Sayı: 1, 71 - 74, 01.04.2013

Öz

Carboxin is one of the most widely used fungicides in agriculture, but information about toxicity on fish is limited.This study assessed the effects of exposure to to carboxin on the antioxidant defence system of rainbow trout (Oncorhynchus mykiss). The fish were exposed to carboxin (3,85 ppm) for seven days. And antioxidant parameter (glutathione reductase, (GR)) was measured in hepar. The results indicated that carboxin exposure significantly affected the activity of GR in fish hepar (p< 0.01). Thus, it was assumed that carboxin caused oxidative stress in rainbow trout and GR enzyme played a role in protection against carboxin toxicity.

Kaynakça

  • EPA. Environmental Protection Agency and Toxic Substances September 2004.
  • CEYHUN, S.B., ŞENTÜRK, M., EKİNCİ, D., ERDOĞAN, O., ÇİLTAŞ, A., KOCAMAN, E.M., Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout, Comp. Biochem. Physiol., 152, 215, 2010.
  • TALAS, Z.S., ORUN, I., OZDEMİR, İ., ERDOĞAN, K., ALKAN, A., YILMAZ, I., Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2, Cr+3) on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792), Fish. Physiol. Biochem., 34, 217, 2008.
  • DOYOTTE, A., COSSU, C., JACQUIN, M.C., BABUTB, M., VASEURAL, P., Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve unio tumidus, Aquat. Toxicol., 39, 93, 1997. ZWART, L.L.D., MEERMAN, J.H.N., COMMANDEUR, J.N.M., VERMEULEN, N.P.E., Biomarkers of free radical damage applications in experimental animals and in humans, Free Radıcal. Bıo. Med., 27, 202, 1999.
  • ORUÇ, E.Ö., SEVGİLER, Y., ÜNER, N., Tissuespecific oxidative stress responses in fish exposed to 2,4D and azinphosmethyl, Comp. Biochem. Phys., 137, 43, 200 ÇINKILOĞLU, E. Cyprinus carpio’da beyin dokusunda fenthionun antioksidant savunma sistemi, lipid peroksidasyonu ve asetilkolinesteraz enzim aktivitesine n-asetilsistein modülatörlüğünde etkileri. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü. 2007.
  • WIEGAND, C., KRAUSE, E., STEINBERG C., PFLUGMACHER, S., Toxicokinetics of atrazine in embryos of the zebrafish (Danio rerio), Ecotoxicol. Environ. Safe.. 49, 199, 2001.
  • RAO,J.V.,Sublethaleffectsofanorganophosphorusinse cticide (RPR- I)onbiochemical parametersoftilapia, Oreochromis mossambicus, Comp.Biochem. Physiol., 143,492, 2006.
  • SETH, V., BANERJEE, B.D., CHAKRAVORTY, A.K., Lipid peroxidation, free radical scavenging enzymes, and glutathione redox system in blood rats exposed to propoxur, Pestıc. Bıochem. Phys., 71, 133, 200 JOS, A., PICHARDO, S., PRIETO, A.I., REPETTO, G., VAZQUEZ, C.M., MORENO, I., CAMEAN, A.M., Toxic cyanobacterial cells containing microcystins induce oxidative stress in exposed tilapia fish (Oreochromis sp.) under laboratory conditions, Aquat. Toxicol., 72, 261, 2005.
  • PEIXOTO, F., ALVES-FERNANDES, D., SANTOS, D., FONTAÍNHASFERNANDES, A., Toxicological Effects of Oxyfluorfen on Oxidative Stress Enzymes in Tilapia Oreochromis niloticus. Pestıc. Bıochem. Phys., 85, 91, 2006.
  • LI, Z., ZLABEKA, V., GRABICA, R., LIA, P., MACHOVAA, J., VELISEKA, J., RANDAK, T., Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na+– K+-ATPase activity in brain of rainbow trout, Oncorhynchus mykiss, Aquat. Toxicol.,98, 297,2010.
  • FIGUEIREDO-FERNANDES,A.,FONTAI´NHASFERNANDES, A., PEIXOTO, F., ROCHA, E., REISHENRIQUES, M.A., Effects of gender and temperature on oxidative stress enzymes in Nile tilapia (Oreochromis niloticus) exposed to paraquat, Pestıc. Bıochem. Phys., 85, 97, 2006.
  • LOPES, P.A., PINHEIRO, T., SANTOS, M.C., MATHIAS, M.D.L., COLLARESPEREIRA, M.J., VIEGAS-CRESPO, A.M., Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides) to inorganic pollutants exposure, Sci. Total Environ., 280, 153, 2001.

EFFECTS OF CARBOXIN ON GLUTATHIONE REDUCTASE ENZYME ACTIVITY IN RAINBOW TROUT (Oncorhynchus mykiss)

Yıl 2013, Cilt: 17 Sayı: 1, 71 - 74, 01.04.2013

Öz

Carboxin is one of the most widely used fungicides in agriculture, but information about toxicity on fish is limited. This study assessed the effects of exposure to to carboxin on the antioxidant defence system of rainbow trout (Oncorhynchus mykiss). The fish were exposed to carboxin (3,85 ppm) for seven days. And antioxidant parameter (glutathione reductase, (GR)) was measured in hepar. The results indicated that carboxin exposure significantly affected the activity of GR in fish hepar (p< 0.01). Thus, it was assumed that carboxin caused oxidative stress in rainbow trout and GR enzyme played a role in protection against carboxin toxicity.

Kaynakça

  • EPA. Environmental Protection Agency and Toxic Substances September 2004.
  • CEYHUN, S.B., ŞENTÜRK, M., EKİNCİ, D., ERDOĞAN, O., ÇİLTAŞ, A., KOCAMAN, E.M., Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout, Comp. Biochem. Physiol., 152, 215, 2010.
  • TALAS, Z.S., ORUN, I., OZDEMİR, İ., ERDOĞAN, K., ALKAN, A., YILMAZ, I., Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2, Cr+3) on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792), Fish. Physiol. Biochem., 34, 217, 2008.
  • DOYOTTE, A., COSSU, C., JACQUIN, M.C., BABUTB, M., VASEURAL, P., Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve unio tumidus, Aquat. Toxicol., 39, 93, 1997. ZWART, L.L.D., MEERMAN, J.H.N., COMMANDEUR, J.N.M., VERMEULEN, N.P.E., Biomarkers of free radical damage applications in experimental animals and in humans, Free Radıcal. Bıo. Med., 27, 202, 1999.
  • ORUÇ, E.Ö., SEVGİLER, Y., ÜNER, N., Tissuespecific oxidative stress responses in fish exposed to 2,4D and azinphosmethyl, Comp. Biochem. Phys., 137, 43, 200 ÇINKILOĞLU, E. Cyprinus carpio’da beyin dokusunda fenthionun antioksidant savunma sistemi, lipid peroksidasyonu ve asetilkolinesteraz enzim aktivitesine n-asetilsistein modülatörlüğünde etkileri. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü. 2007.
  • WIEGAND, C., KRAUSE, E., STEINBERG C., PFLUGMACHER, S., Toxicokinetics of atrazine in embryos of the zebrafish (Danio rerio), Ecotoxicol. Environ. Safe.. 49, 199, 2001.
  • RAO,J.V.,Sublethaleffectsofanorganophosphorusinse cticide (RPR- I)onbiochemical parametersoftilapia, Oreochromis mossambicus, Comp.Biochem. Physiol., 143,492, 2006.
  • SETH, V., BANERJEE, B.D., CHAKRAVORTY, A.K., Lipid peroxidation, free radical scavenging enzymes, and glutathione redox system in blood rats exposed to propoxur, Pestıc. Bıochem. Phys., 71, 133, 200 JOS, A., PICHARDO, S., PRIETO, A.I., REPETTO, G., VAZQUEZ, C.M., MORENO, I., CAMEAN, A.M., Toxic cyanobacterial cells containing microcystins induce oxidative stress in exposed tilapia fish (Oreochromis sp.) under laboratory conditions, Aquat. Toxicol., 72, 261, 2005.
  • PEIXOTO, F., ALVES-FERNANDES, D., SANTOS, D., FONTAÍNHASFERNANDES, A., Toxicological Effects of Oxyfluorfen on Oxidative Stress Enzymes in Tilapia Oreochromis niloticus. Pestıc. Bıochem. Phys., 85, 91, 2006.
  • LI, Z., ZLABEKA, V., GRABICA, R., LIA, P., MACHOVAA, J., VELISEKA, J., RANDAK, T., Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na+– K+-ATPase activity in brain of rainbow trout, Oncorhynchus mykiss, Aquat. Toxicol.,98, 297,2010.
  • FIGUEIREDO-FERNANDES,A.,FONTAI´NHASFERNANDES, A., PEIXOTO, F., ROCHA, E., REISHENRIQUES, M.A., Effects of gender and temperature on oxidative stress enzymes in Nile tilapia (Oreochromis niloticus) exposed to paraquat, Pestıc. Bıochem. Phys., 85, 97, 2006.
  • LOPES, P.A., PINHEIRO, T., SANTOS, M.C., MATHIAS, M.D.L., COLLARESPEREIRA, M.J., VIEGAS-CRESPO, A.M., Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides) to inorganic pollutants exposure, Sci. Total Environ., 280, 153, 2001.
Toplam 12 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Mühendislik
Bölüm Araştırma Makalesi
Yazarlar

Gonca Alak Bu kişi benim

Muhammed Atamanalp Bu kişi benim

Esat Mahmut Kocaman Bu kişi benim

Arzu Uçar Bu kişi benim

Yayımlanma Tarihi 1 Nisan 2013
Gönderilme Tarihi 18 Ekim 2012
Kabul Tarihi 20 Ocak 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 17 Sayı: 1

Kaynak Göster

APA Alak, G., Atamanalp, M., Kocaman, E. M., Uçar, A. (2013). KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ. Sakarya University Journal of Science, 17(1), 71-74. https://doi.org/10.16984/saufbed.85782
AMA Alak G, Atamanalp M, Kocaman EM, Uçar A. KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ. SAUJS. Nisan 2013;17(1):71-74. doi:10.16984/saufbed.85782
Chicago Alak, Gonca, Muhammed Atamanalp, Esat Mahmut Kocaman, ve Arzu Uçar. “KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus Mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ”. Sakarya University Journal of Science 17, sy. 1 (Nisan 2013): 71-74. https://doi.org/10.16984/saufbed.85782.
EndNote Alak G, Atamanalp M, Kocaman EM, Uçar A (01 Nisan 2013) KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ. Sakarya University Journal of Science 17 1 71–74.
IEEE G. Alak, M. Atamanalp, E. M. Kocaman, ve A. Uçar, “KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ”, SAUJS, c. 17, sy. 1, ss. 71–74, 2013, doi: 10.16984/saufbed.85782.
ISNAD Alak, Gonca vd. “KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus Mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ”. Sakarya University Journal of Science 17/1 (Nisan 2013), 71-74. https://doi.org/10.16984/saufbed.85782.
JAMA Alak G, Atamanalp M, Kocaman EM, Uçar A. KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ. SAUJS. 2013;17:71–74.
MLA Alak, Gonca vd. “KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus Mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ”. Sakarya University Journal of Science, c. 17, sy. 1, 2013, ss. 71-74, doi:10.16984/saufbed.85782.
Vancouver Alak G, Atamanalp M, Kocaman EM, Uçar A. KARBOKSİNİN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss) GULUTATYON REDÜKTAZ ENZİM AKTİVİTESİ ÜZERİNE ETKİSİ. SAUJS. 2013;17(1):71-4.

30930 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.