Effects of Food Based Yeast on Oxidant-Antioxidant Systems in Rats fed by High Cholesterol Diet

Abstract

In living organisms, oxidant and antioxidant systems are in a balance. In the present study, our aim was to study the effects of Cryptococcus humicola, which is a food based yeast whose cholesterol lowering activity is under investigation, on oxidant and antioxidant systems.

31 adult male, Wistar albino rats weighing 200-250 gr were included in the study. Rats were divided into four groups based on their diets. Group 1(Control Group) was fed a normal diet, Group 2 was fed a high cholesterol diet, Group 3 was fed a high cholesterol and low dose yeast diet (0.1% lyophilysed yeast), and Group 4 was fed a high cholesterol and high dose yeast diet (2% lyophilysed yeast). After fifty six days, bloods were taken and serums were collected. Total antioxidant capacity (TAS) and total oxidant capacity (TOS) levels were determined through Erel method.

When TAS, TOS and OSI values were compared by nonparametric Kruskall Wallis test, no significant difference was found among the groups. 

This may lead to questioning the efficiency of the diet. It may be possible to show the antioxidant activity of c. humicola by increasing the yeast dose, number of subjects and duration of the experiment.

Keywords

• Antioxidant,Cholesterol,Yeast,Oxidative stress

References

1. Amdekar S, Singh V, Kumar A, Sharma P, Singh R. 2013. Lactobacillus casei and Lactobacillus acidophilus regulate inflammatory pathway and improve antioxidant status in collagen-induced arthritic rats. J Interferon Cytokine Res. 33(1): 1-8.
2. Anwar H, Rahman ZU, Javed I, Muhammad F. 2012. Effect of protein, probiotic, and symbiotic supplementation on serum biological health markers of molted layers. Poult Sci 91(10): 2606-2613.
3. Awaisheh SS, Khalifeh MS, Al-Ruwaili MA, Khalil OM, Al-Ameri OH, Al-Groom R. 2013. Effect of supplementation of probiotics and phytosterols alone or in combination on serum and hepatic lipid profiles and thyroid hormones of hypercholesterolemic rats. J Dairy Sci. 96(1): 9-15.
4. Belet N. 2011. Çocuklarda Nadir Görülen İnvazif Mantar Enfeksiyonları. J Pediatr Inf 5 (Suppl 1), 183-187.
5. Benyacoub J, Rochat F, Saudan KY.2008. Feeding a diet containing a fructooligosaccharide mix can anhance Salmonella vacine efficacy in mice. J Nutr 138: 123-129
6. Bockelmann W, Heller M, Heller KJ. 2008. Identification of Yeasts of Dairy Origin by Amplified Ribosomal DNA Restriction Analysis (ARDRA), Int Dairy J. 18: 1066-1071.
7. Boehm G, Lidestri M, Casetta P. 2002. Supplementation of a bovine milk Formula with an oligosaccharide mixture increases counts of faecal bifidobacteria in preterm infants. Arch Dis Child Fetal Neonatal Ed 86: 78-81.
8. Bruzzese E, Volpicelli M, Salvini F. 2005. Early administration of GOS/ FOS prevents intestinal and respiratory infections in infants. J Pediatr Gastroenterol Nutr 40: 36-42

9. Erel O. (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 37:277–285.
10. Esen E. 2008. Farklı Gıda Maddelerinde Maya Kontaminasyonu Üzerine Bir Çalışma (Yüksek Lisans Tezi). İstanbul Üniversitesi Sağlık Bilimleri Ensitüsü. Besin Hijyeni ve Teknolojisi Anabilim Dalı. Istanbul. Turkey. Pp 15-22.
11. Giorgi PL. 2009. Probiotics a review. Recenti Prog Med. 100(1):40-47.
12. Halliwell B, Gutteridge JMC. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219: 1-4
13. Hanie S. Ejtahed M. Javad Mohtadi-Nia, Aziz Homayouni-Rad. Mitra Niafar, Mohammad Asghari-Jafarabadi, Vahid Mofid, 2012. Probiotic yogurt improves antioxidant status in type 2 diabetic patients. Nutrition 28: 539–543
14. Havenaar R, Huis I’ntVeld MJH. 1992. Probiotics: a general view. In: Lactic acid bacteria in health and disease. Vol 2. Elsevier Applied Sciences Publishers. Amsterdam. Pp 8-15.
15. Isolauri E. 2004. The role of probiotics in pediatrics. Curr Pediatr 24: 104-109.
16. Kurtzman CP, Fell JW, Boekhout T. 2011. The yeasts a taxonomic study. Fifth Edition. Elsevier. London. pp 22-36.
17. Mahfouz MM, Kumerrow FA. 2000. Cholesterol-rich diets have different effects on lipid peroxidation, cholesterol oxides and antioxidant enzymes in rats and rabbits. J Nutr Biochem 11: 293-302.
18. Metehan Ö. 2011. Sağlıklı kalmak için probiyotikler, prebiyotikler anlatılmayan tarihçe. Nobel Tıp Kitapevleri. İstanbul. Türkiye. p1-11
19. Simova E, Beshkova D, Angelov A, Hristozova T.S., Frengova G, Spasov Z. 2002. Lactic Acid Bacteria and Yeasts in Kefir Grains and Kefir Made from Them. J Ind Microbiol Biotechnol 28: 1-6.
20. Vasile N, Ghindea R, Vassu T. 2011. Probiotics an alternative treatment for various diseases. Roum Arch Microbiol Immunol. 70(2): 54-59.
21. Zhang J, Harbottle G, Wang C, Kong Z. 1999. Oldest playable musical instruments found at Jiahuearly Neolithic site in China. Nature 401:366-8.