Sudak (Stizostedion lucioperca L., 1758) Balığının Gastrointestinal Kanalının Histokimyasal Yapısı

Abstract

Özet: Bu çalışmada sudak (Stizostedion lucioperca) balığının mide ve bağırsak bölgelerinin histokimyasal yapısının belirlenmesi amaçlanmaktadır. Sudakların mide (kardiya, fundus, pilorus) ile ilk, orta ve son bağırsaklarından doku örnekleri alınıp, gastrointestinal mukosubstans özelliklerinin belirlenmesi için histokimyasal boyama yöntemleri uygulandı. Uygulanan histokimyasal boyama yöntemlerinin, sudak balığının çalışılan tüm bölgelerinde reaksiyon verdiği saptandı. Asidik mukosubstansın mide bölgelerinde, nötr ve sülfatlı mukosubstansın ise bağırsaklarda fazla olduğu belirlendi. AB pH 1.0, AB pH 0.5, AB 0.06 M ve AB 0.3 M uygulamalarında reaksiyonun oldukça zayıf olduğu saptandı.

Keywords

• Histokimya, mukosubstans, sudak (Stizostedion lucioperca)

Histochemical Structure of Gastrointestinal Tract of Zander (Stizostedion lucioperca L., 1758)

Abstract

Abstract: Purpose of this study was to determine the histochemical structure of zander (Stizostedion lucioperca) on stomach and intestines. Tissue samples were taken from stomach (cardiac, fundus, pylorus), anterior, middle and posterior intestine and performed histochemical staining methods for determination of gastrointestinal mucosubstance. The histochemical stain methods were found to react in all studied parts of zander. The acidic mucosubstance was too much in the stomach, while the neutral and sulphated mucosubstance were found to be too much in the intestine. AB pH 1.0, AB pH 0.5, AB 0.06 M and AB 0.3 practices were identified as relatively weak reaction.

Keywords

• Histochemical,mucosubstance,zander (Stizostedion lucioperca)

References

1. Albrecht M.P., Ferreira M.F.N., Caramaschi E.P., 2001. Anatomical features and histology of the digestive tract of two related neotropical omnivorous fishes (Characiformes; Anostomidae), Journal of Fish Biology, 58(2): 419–430.
2. Cataldi E., Cataudella S., Monaco G., Rossi A., Tancioni L., 1987. A study of the histology and morphology of the digestive tract of Sea bream, Sparus aurata, Journal of Fish Biology, 30(1): 135–145.
3. Grau A., Crespo S., Sarasguete N.C., Gonzales d’Canales N.L., 1992. The digestive tract of rhe Amberjack, Seriola dumerili, Risso: A light and scanning electron microscope study, Journal of Fish Biology, 41: 387–390.
4. Martin T.J., Blaber S.J.M., 1984. Morphology and histology of the alimentary tract of Ambassidae (Teleostei) in relation to feding, Journal of Morphology, 182: 295–305.
5. Sis R.F., Ives P.J., Jones D.M., Haensly D.H., 1979. The microscopic anatomy of the oesophagos, stomach and intestine of the Channel Catfish, Ictalurus punctatus, Journal of Fish Biology, 14: 179–186.
6. Zhu Y., Zhang Q., 1993. On feeding habits and histological structure of digestive tract of the Mudskipper, Beleophthalmus pectinirostris, in interdial zone of iulong rivereustary, Journal of Ocean Taiwan, 12(3): 225-232.
7. Diaz A.O., Garcia A.M., Devincenti C.V., Goldemberg A.L., 2003. Morphological and histochemical characterization of the mucosa of the digestive tract in Engraulis anchoita, Anatomia Histologia Embryologia, 32: 341–346.
8. Diaz A.O., Garcia A.M., Figueroa D.E., Goldemberg A.L., 2008. The mucosa of digestive tract in Micropogonias furnieri: A light and electron microscope approach, Anatomia Histologia Embryologia, 37: 251–256.

9. Murray H.M., Wright G.M., Goff G.P., 1996. A comparative histological and histochemical study of the post-gastric alimentary canal from three species of pleuronectid, the Atlantic halibut, the yellowtail flounder and the winter flounder, Journal of Fish Biology, 48: 187–206.
10. Perez-Vilar J., Hill R.L., 2007. Mucin granule intraluminal organization, Respiratory Cell and Molecular Biology, 36: 183-190.
11. Culling C.F.A., Reid P.E., Dunn W.L., 1976. A new histochemical method for the identification and visualization of both side chain acylated and non-acylated sialic acids, Journal of Histochemistry and Cytochemistry, 24: 1225–1230.
12. Bancroft J.D., Stevens A., Turner D.R., 1996. Theory and practice of histological techniques, Churchill Livingstone, London, p. 129.
13. Boulhic M., Gabaudan J., 1992. Histological study of the organogenesis of the digestive system and swim bladder of the Docer Sole, Solea solea (Linneaus 1758), Aquaculture, 102: 373–396.
14. Osman A.H.K., Caceci T., 1991. Histology of the stomach of Tilapia nilotica (Linnaeus 1758) from the River Nile, Journal of Fish Biology, 38: 212–223.
15. Reid P.E., Volz D., Cho K.Y., Owen D.A., 1988. A new method for the histochemical demonstration of o-acyl sugars in human colonic epithelial glycoproteins, Histochemistry Journal, 20: 510–518.
16. Domeneghini C., Arrighi S., Radaelli G., Bosi G., Mascarello F., 1998. Morphological and histochemical peculiarities od the gut in the white sturgeon Acipenser transmontanus, European Journal of Histochemistry, 43: 135–145.
17. Çelikkale M.S., 1991. Balık Biyolojisi, Karadeniz Teknik Üniversitesi Sürmene Deniz Bilimleri ve Teknolojisi Yüksekokulu Yayınları, No:101, Trabzon, 387s.
18. Lev R., Spicer S.S., 1964. Specific staining of sulphate groups with alcian blue at low pH, Journal of Histochemistry and Cytochemistry, 12: 309.
19. Scott J.E., Dorling J., 1965. Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions, Histochemistry, 21: 277–285.
20. Gomori, 1952. Gomori’s aldehyde fuchsin stain. In, Culling CFA, Allison RT, Barr WT (Ed): Cellular Pathology Technique, Butterworths, London, p 238-240.
21. Spicer Mayer, 1960. Aldehyde Fuchsin/Alcian Blue. In, Culling C.F.A., Allison R.T., Barr W.T (Ed), Cellular Pathology Technique, Butterworths, London, p 233.
22. McManus J.F.A., 1948. Histological and histochemical uses of periodic acid, Stain Technology, 23: 99–108.
23. Mowry R.W.,1956. Alcian blue techniques for the histochemical study of acidic carbohydrates, Journal of Histochemistry and Cytochemistry, 4: 407–408.
24. Burrin D.G., Stoll B., Guan X., 2003. Glucagon-like peptide 2 function in domestic animals, Domestic Animal Endocrinology, 24(2): 103–122.
25. Neuhaus H., Van Der Marel M., Caspari N., Meyer W., Enss M.L., Steinhagen D., 2007. Biochemical and histochemical study on the intestinal mucosa of the common carp Cyprinus carpio L., with special consideration of mucin glycoproteins, Journal of Fish Bioogyl, 70: 1523– 1534.
26. Pedini V., Scocco P., Radaelli G., Fagioli O., Ceccarelli P., 2001: Carbohydrate histochemistry of the alimentary canal of the Shi Drum, Umbrina cirrosa L, Anatomia Histologia Embryologia, 30: 345-349.
27. Ferri D., Liquori G.E., Scillitani G., 1999. Morphological and histochemical variations of mucous and oxynticopeptic cells in the stomach of the seps, Chalcides chalcides, Journal of Anatomy, 194: 71–77.
28. Ribeiro L., Sarasquete C., Dinis M.T., 1999. Histological and histochemical development of the digestive system of Solea senegalensis larvae, Aquaculture, 171: 293–308.
29. Gisbert E., Sarasquete M.C., Williot P., Castello-Orvay F., 1999. Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny, Journal of Fish Biology, 55: 596–616.
30. Tibbetts I.R., 1997. The distribution and function of mucous cells and their secretions in the alimentary tract of Arrhamphus sclerolepis krefftii, Journal of Fish Biology, 50: 809–820.
31. Domeneghini C., Radaelli G., Bosi G., Arrighi S., Giancamillo A.D., Pazzaglia M., Mascarello F., 2002. Morphological and histochemical differences in the structure of the alimentary canal in feding and runt (feed deprived) white sturgeons (Acipenser transmontanus). Journal of Applied Ichthyology, 18: 341–346.
32. Scocco P., Menghi G., Ceccarelli P., 1997. Histochemical differentiation of glycoconjugates occurring in the tilapine intestine, Journal of Fish Biology, 51: 848–857.
33. Park J.Y., Kim I.S., Kim S.Y., 2003. Structure and mucous histochemistry of the intestinal respiratory tract of the mud loach, Misgurnus anguillicaudatus (Cantor), Journal of Applied Ichthyoogyl, 19: 215–219.
34. Ülker Eren e-posta: [e-mail]
35. Kenan Çınar e-posta: [e-mail]