Farklı Hücre Hatlarında Besiyeri ve FBS’in Hücre Proliferasyonu Üzerindeki Etkilerinin İncelenmesi

Abstract

Hücre kültürü, laboratuvar şartlarında hücrelerin canlılığının ve proliferasyonunun devam ettirilmesi için oluşturulan yapay, steril bir ortamdır. Bu yapay ortam şartlarında, hücrelerin büyümelerinin desteklenmesi amacıyla besiyerine ek olarak fetal bovine serum (FBS) gibi hücre proliferasyonunu arttırıcı maddeler de eklenmektedir. Hücreler uygun besiyeri ve FBS oranı kullanıldığı takdirde daha etkili bir şekilde prolifere olabilmektedir. Bu çalışmada, farklı besiyeri çeşitlerinin (Dulbecco's Modified Eagle's Medium (DMEM), RPMI-1640, DMEM High-Glucose ve DMEM/F12) ve farklı FBS oranlarının (%0, %5, %10, %15, %20, %25) belirlenen hücre hatlarındaki (L929, J774, Arpe-19, RVECs, AU-565, MCF-7, HEK293T) hücre proliferasyonuna etkileri incelendi. Hücre canlılık analizleri MTT testi ile yapıldı. MTT testi sonuçlarına göre belirlenen hücre hatlarının prolifere olacağı en uygun besiyeri çeşidi ve kullanılacak FBS oranları belirlendi. Bu çalışmayla birlikte, belirtilen hücre hatlarının birçok besiyeri çeşidinde ve farklı FBS oranlarında prolifere olabileceği gözlemlenmiştir. Elde edilen veriler dikkate alındığında, farklı hücre hatlarında optimum hücre proliferasyonun gerçekleştiği besiyeri çeşidi ve maliyeti yüksek ürünlerden biri olan FBS’in optimum hücre proliferasyonunu sağladığı ideal düzeydeki kullanım oranları saptanmıştır.

Keywords

• Hücre Kültürü
• Besiyeri
• FBS
• MTT
• Proliferasyon

Investigation of the Effects of Medium and FBS on Cell Proliferation in Different Cell Lines

Abstract

Cell culture is an artificial, sterile environment created to maintain the viability and proliferation of cells under laboratory conditions. In these artificial environment conditions, in addition to the medium, cell proliferation enhancing substances such as Fetal bovine serum (FBS) are added to support the growth of cells. Cells can proliferate more effectively if appropriate medium and FBS ratio are used. In this study, it was determined that different media types (Dulbecco's Modified Eagle's Medium (DMEM), RPMI-1640, DMEM High-Glucose and DMEM/F12) and different FBS ratios (0%, 5%, 10%, 15%, 20%, 25%) effects on cell proliferation in determined cell lines (L929, J774, Arpe-19, RVECs, AU-565, MCF-7, HEK293T) were examined. Cell viability analyzes were performed with the MTT test. According to the results of the MTT test, the most suitable medium type on which the cell lines will proliferate and the FBS ratios to be used were determined. With this study, it was determined that the specified cell lines could proliferate in many media types and at different FBS ratios. When cell studies are carried out considering the study data, the production of cells will be carried out using the most effective medium type for different cell lines and the FBS ratio at which optimum cell proliferation occurs. In this way, it is aimed to produce cells by using FBS, which is one of the most costly products in cell studies, at the most ideal level.

Keywords

• Cell Culture
• Medium
• FBS
• MTT
• Proliferation

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