Yıl 2016, Cilt 11 , Sayı 1, Sayfalar 72 - 81 2016-05-24

Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form

Zehra ÜSTÜN [1] , Aybike TALAY [2] , Ebru ÇUBUK DEMİRALAY [3]


Abstract: In this study, a simple reverse phase liquid chromatographic (RPLC) method has been developed and subsequently validated for simultaneous determination of atorvastatin (ATV) and rosuvastatin (RSV). The column used was X Terra C18, (250 mm x 4.6 mm I.D., 5µm) with flow rate of 1 mL min-1 using photodiode array detection at 244 nm. The described method was linear over a concentration range of 3-13 µg mL-1 and 4-14 µg mL-1 for the assay of ATV and RSV respectively. Losartan was used as internal standard (IS) in the experiment. The limit of detection (LOD) values for ATV and RSV were found to be 0.133 and 0.221 µg mL-1 respectively. Limit of quantification (LOQ) for ATV and RSV were found to be 0.473 and 0.670 µg mL-1, respectively. The results of the study showed that the proposed RPLC method is useful for the routine determination of ATV and RSV and in their pharmaceutical dosage form. Keywords: Method optimization, validation, simultaneous determination, atorvastatin, rosuvastatin İlaç Dozaj Formunda Atorvastatin ve Rosuvastatinin kantitatif analizi için RPLC koşullarının optimizasyonu Özet: Bu çalışmada, atorvastatin (ATV) ve rosuvastatinin (RSV) eş zamanlı tayini için ters faz sıvı kromatografi (RPLC) metodu geliştirilmiş ve valide edilmiştir. Ayırmada X Terra C18 (250 mm x 4,6 mm, 5µm) kolon kullanılmış ve 244 nm’ de yürütülen çalışmada akış hızı 1 mL dakika-1 olarak belirlenmiştir. Tanımlanan metodun doğrusal aralığı ATV için 3-13 µg mL-1, RSV için 4-14 µg mL-1olarak belirlenmiştir. Deneyde iç standart (IS) olarak Losartan kullanılmıştır. ATV ve RSV için dedeksiyon limiti (LOD) değerleri sırasıyla 0,133 ve 0,221 µg mL-1 olarak hesaplanmıştır. Kantitasyon limiti (LOQ) değerleri ise ATV ve RSV için sırasıyla 0,473 ve 0,970 µg mL-1 olarak tayin edilmiştir. Elde edilen sonuçlara bakılarak, önerilen RPLC metodu ATV, RSV ve bunların farmasötik dozaj formlarının rutin analizlerinde kullanılabilir olduğu gözlemlenmiştir. Anahtar Kelimeler: Metot optimizasyonu, validasyon, eş zamanlı tayin, atorvastatin, rosuvastatin
Method optimization, validation, simultaneous determination, atorvastatin, rosuvastatin
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Birincil Dil en
Konular Mühendislik, Kimya, Biyoloji
Bölüm Makaleler
Yazarlar

Yazar: Zehra ÜSTÜN

Yazar: Aybike TALAY

Yazar: Ebru ÇUBUK DEMİRALAY

Tarihler

Yayımlanma Tarihi : 24 Mayıs 2016

Bibtex @araştırma makalesi { sdufeffd270035, journal = {Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi}, issn = {}, eissn = {1306-7575}, address = {}, publisher = {Süleyman Demirel Üniversitesi}, year = {2016}, volume = {11}, pages = {72 - 81}, doi = {}, title = {Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form}, key = {cite}, author = {ÜSTÜN, Zehra and TALAY, Aybike and ÇUBUK DEMİRALAY, Ebru} }
APA ÜSTÜN, Z , TALAY, A , ÇUBUK DEMİRALAY, E . (2016). Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form. Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi , 11 (1) , 72-81 . Retrieved from https://dergipark.org.tr/tr/pub/sdufeffd/issue/25595/270035
MLA ÜSTÜN, Z , TALAY, A , ÇUBUK DEMİRALAY, E . "Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 11 (2016 ): 72-81 <https://dergipark.org.tr/tr/pub/sdufeffd/issue/25595/270035>
Chicago ÜSTÜN, Z , TALAY, A , ÇUBUK DEMİRALAY, E . "Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 11 (2016 ): 72-81
RIS TY - JOUR T1 - Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form AU - Zehra ÜSTÜN , Aybike TALAY , Ebru ÇUBUK DEMİRALAY Y1 - 2016 PY - 2016 N1 - DO - T2 - Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi JF - Journal JO - JOR SP - 72 EP - 81 VL - 11 IS - 1 SN - -1306-7575 M3 - UR - Y2 - 2019 ER -
EndNote %0 Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form %A Zehra ÜSTÜN , Aybike TALAY , Ebru ÇUBUK DEMİRALAY %T Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form %D 2016 %J Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi %P -1306-7575 %V 11 %N 1 %R %U
ISNAD ÜSTÜN, Zehra , TALAY, Aybike , ÇUBUK DEMİRALAY, Ebru . "Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form". Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi 11 / 1 (Mayıs 2016): 72-81 .
AMA ÜSTÜN Z , TALAY A , ÇUBUK DEMİRALAY E . Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form. SDÜFEFFD. 2016; 11(1): 72-81.
Vancouver ÜSTÜN Z , TALAY A , ÇUBUK DEMİRALAY E . Optimization of RPLC Conditions for Quantitative Analysis of Atorvastatin and Rosuvastatin in Pharmaceutical Dosage Form. Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi. 2016; 11(1): 81-72.