Dışsal Glisin Uygulamasıyla Allium cepa L.'da Tuz Teşvikli Stresin Hafifletilmesi

Year 2019, Volume: 14 Issue: 2, 248 - 255, 30.11.2019

Dilek Çavuşoğlu

https://doi.org/10.29233/sdufeffd.578557

Abstract

Bu çalışmada, tuzluluğa maruz bırakılan Allium cepa L. tohumlarındaki
fizyolojik parametreler olarak tohum çimlenmesi,
radikula uzunluğu, radikula sayısı, taze ağırlık ve sitogenetik parametreler olarak mitotik aktivite, kromozomal anormallikler ve
mikronükleus sıklığı üzerine glisinin rolü incelenmiştir. Tuzluluk A. cepa'nın tohum çimlenmesi ve fide
büyümesinde önemli bir inhibe edici etki göstermiştir. Dahası,
tuz A. cepa’nın kök ucu
hücrelerindeki mitotik indeksi önemli ölçüde düşürmüş ve sitolojik hasarın en
basit ve en etkili göstergesi olan mikronükleus sıklığı ve kromozomal
anormalliklerin sayısını arttırmıştır. Buna karşılık, tohum çimlenmesi,
mitotik aktivite, kromozomal anormallikler ve fide büyümesi üzerine tuzun
inhibe edici etkileri glisin uygulamasıyla önemli ölçüde azalmış, fakat
mikronükleus sıklığı üzerinde glisin tuz hasarının azaltılmasında yetersiz
kalmıştır.

Keywords

Glisin, Tohum çimlenmesi, Fide büyümesi, Mitotik indeks, Kromozomal anormallikler, Tuz stresi

Alleviation of Salt-Induced Stress in Allium cepa L. by Exogenous Glycine Treatment

Abstract

In
this study, the role of glycine on the mitotic index, chromosome aberrations, micronucleus
frequency as cytogenetic parameters
and on the seed germination, radicle length, radicle number, fresh weight
as physiological parameters in Allium cepa L. seeds exposed to saltness
were studied. Salinity displayed
a significant inhibitory effect on the seedling growth and seed germination of Allium cepa. Furthermore, salinity
reduced significantly the mitotic index in A. cepa root tip cells and ascended number of chromosomal aberrations
and frequency
of micronucleus which is the simplest
indicator, the most effective of cytological damage. On the other hand, the restricting effects
of salinity on the seed germination, mitotic activity, chromosomal aberrations and seedling growth were alleviated dramatically in
varying degrees by glycine application, but glycine was ineffective in reducing
of salt damage on the micronucleus
frequency.

Keywords

Glycine, Seed germination, Seedling growth, Mitotic index, Chromosomal aberrations, Salt stress

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