THE POSSIBLE ROLE OF MIR-1910-3P, MIR-4649-3P, MIR-4296, AND MIR-210 IN THE PATHOGENESIS OF ATOPIC DERMATITIS: MAY MIR-4296 PLAY CRUCIAL ROLES IN THE DEVELOPMENT OF ATOPIC DERMATITIS?

Öz

Objective Atopic dermatitis (AD) is a chronic inflammatory skin disease with unexplained points in its pathogenesis. Altered expressions of microRNAs (miRNA, miR) in plasma can serve as markers that distinguish diseased individuals from healthy controls AD. In the present study, plasma expression levels of miR-1910-3p, miR- 4649-3p, miR-4296 and miR-210 were investigated in AD. Material and Method Forty patients with AD and forty healthy control subjects were included in the present study. Quantitative realtime PCR was used to measure miRNAs. Results The mean plasma miR-4296 level was higher in the patient group (p < 0.001). There was a significant negative correlation between SCORAD scores and miR-210 levels (r:-0.340, p=0.032). miR-210 levels decreased with increasing disease severity. In logistic regression analyses, an increase in plasma miR-4296 levels was found to be statistically significant (OR =5.464, p<0.001). Moreover, although not significant in other analyzes, a decrease in the miR-1910-3p was shown statistically significant. Conclusion MiRNAs are crucial in the pathogenesis of AD. Increased miR-4296 seems to be significantly better at discriminating AD patients from healthy individuals.

Anahtar Kelimeler

• Atopic dermatitis
• miR-4296
• miR-1910-3p
• miR-210
• SCORAD

ATOPİK DERMATİT PATOGENEZİNDE MİR-1910-3P, MİR-4649-3P, MİR-4296 VE MİR-210'UN OLASI ROLÜ: MİR-4296 ATOPİK DERMATİT GELİŞİMİNDE ÖNEMLİ ROL OYNAYABİLİR Mİ?

Öz

Amaç Atopik dermatit (AD), patogenezinde açıklanamayan noktaları olan kronik inflamatuar bir deri hastalığıdır. Plazmadaki mikroRNA'ların (miRNA, miR) değişmiş ekspresyonları, hastalıklı bireyleri sağlıklı kontrollerden ayıran belirteçler olarak hizmet edebilir. Bu çalışmada, AD’li hastalarda miR-1910-3p, miR-4649-3p, miR-4296 ve miR-210'un plazma ekspresyon düzeyleri araştırıldı. Gereç ve Yöntem Bu prospektif çalışmaya 40 AD'li hasta ve 40 sağlıklı kontrol alındı. MiRNA'ları ölçmek için real-time PCR kullanıldı. Bulgular Ortalama plazma miR-4296 düzeyi hasta grupta daha yüksek bulundu (p < 0.001). SCORAD skorları ile miR-210 seviyeleri arasında anlamlı bir negatif korelasyon saptandı (r:-0.340, p=0.032). miR-210 seviyeleri hastalık şiddeti arttıkça azalmaktaydı. Lojistik regresyon analizinde plazma miR-4296 seviyelerinde bir artış istatistiksel olarak anlamlı bulundu (OR =5.464, p<0.001). Diğer analizlerde anlamlı bir farklılık bulunmasa da miR-1910-3p seviyelerinde bir azalma da istatistiksel olarak anlamlıydı. Sonuç MiRNA'lar AD patogenezinde önemli rol oynamaktadırlar. Artan miR-4296 ekspresyon seviyeleri, AD hastalarını sağlıklı kişilerden ayırt etmede önemli ölçüde daha iyi görünmektedir.

Anahtar Kelimeler

• Atopik dermatit
• miR-4296
• miR-1910-3p
• miR-210
• SCORAD

Kaynakça

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