THE MMP9 −1562 C/T POLYMORPHISM IS ASSOCIATED WITH INCREASED RISK OF DIABETIC RETINOPATHY IN TURKISH TYPE 2 DIABETES MELLITUS PATIENTS

Year 2023, , 217 - 224, 22.06.2023

Fadime Mutlu İçduygu , Egemen Akgün , Ebru Alp , Sibel Doğuizi , Murat Atabey Özer

https://doi.org/10.17343/sdutfd.1268838

Abstract

Objective
Type 2 diabetes mellitus (T2DM) and diabetic
retinopathy (DR) development is affected by genetic
factors. Matrix metalloproteinases (MMPs) are involved
in many cellular processes, such as remodeling of
extracellular matrix proteins and angiogenesis. In
this study, we investigated the relationship between
MMP9 −1562 C/T (rs3918242) polymorphism and the
development of T2DM and DR in Turkish population.
Material and Method
A total of 510 participants, involving 168 T2DM
cases with DR, 168 T2DM cases without DR,
and 174 controls, were included in the study. The
polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) method was carried out
to identify genotypes.
Results
The CT, CT+TT genotypes and the T allele of the
MMP9 −1562 C/T polymorphism were associated with
increased risk of DR (p=0.001). On the other hand,
no relationship was found between the development
of T2DM and this polymorphism. In addition,
CT (p=0.010) and CT+TT (p=0.015) genotype
frequencies were found to be higher in proliferative
diabetic retinopathy (PDR) cases compared to nonproliferative
diabetic retinopathy (NPDR) cases, but
after regression analysis, only insulin use (p=0.003)
was found to be associated with the development of
PDR.
Conclusion
Our data show that the MMP9 −1562 C/T polymorphism
is associated with the development of DR in Turkish
T2DM patients, but no significant relationship was
found between this polymorphism and the severity of
DR and the development of T2DM.

Keywords

MMP9, Diabetic retinopathy, proliferative diabetic retinopathy, type 2 diabetes mellitus, polymorphism

MMP9 −1562 C/T POLİMORFİZMİ, TÜRK TİP 2 DİABETES MELLİTUS HASTALARINDA ARTMIŞ DİYABETİK RETİNOPATİ RİSKİ İLE İLİŞKİLİDİR

Abstract

Amaç
Tip 2 diabetes mellitus (T2DM) ve diyabetin mikrokomplikasyonlarından
olan diyabetik retinopati (DR)
gelişimi genetik faktörlerden etkilenmektedir. Matriks
metalloproteinazlar (MMPs), ekstraselüler matriks
proteinlerinin yeniden şekillendirilmesi ve anjiyogenez
gibi pek çok hücresel proseste rol alırlar. Bu çalışmada
Türk toplumunda MMP9 −1562 C/T (rs3918242)
polimorfizmi ile T2DM ve DR gelişim riski arasındaki
ilişkinin araştırılması amaçlanmıştır.
Gereç ve Yöntem
Mevcut çalışmaya 168 DR’si olan T2DM hastası, 168
DR’si olmayan T2DM hastası ve 174 kontrol olmak
üzere toplam 510 birey dahil edilmiştir. Genotipleri belirlemek
amacıyla Polimeraz zincir reaksiyonu-Restriksiyon
fragment uzunluk polimorfizmi (PCR-RFLP)
yöntemi kullanılmıştır.
Bulgular
T2DM gelişimi ile MMP9 −1562 C/T polimorfizmi arasında
bir ilişkiye rastlanmazken, CT ve CT+TT genotipleri
ile T (p=0,001) alleli artmış DR riski ile ilişkili bulunmuştur.
Ayrıca CT (p=0,010) ve CT+TT (p=0,015)
genotip sıklığı proliferatif diyabetik retinopati (PDR)
hastalarında proliferatif olmayan diyabetik retinopati
(NPDR) hastalarına kıyasla yüksek bulunmuş, fakat
regresyon analizi sonrasında sadece insülin kullanımının
(p=0,003) PDR gelişimi ile ilişkili olduğu belirlenmiştir.
Sonuç
Elde ettiğimiz veriler Türk T2DM hastalarında MMP9
−1562 C/T polimorfizminin DR gelişimi ile ilişkili olduğunu,
fakat bu polimorfizm ile DR şiddeti ve T2DM
gelişimi arasında anlamlı bir ilişki bulunmadığını göstermektedir.

Keywords

Diabetik retinopati, MMP9, Polimorfizm, Proliferatif diyabetik retinopati, Tip 2 diabetes mellitus

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