SUBARAKNOİD KANAMANIN NEDEN OLDUĞU SEREBELLAR HASARDA UYGULANAN FARKLI TEDAVİ MODALİTELERİNİN OKSİDATİF STRES PARAMETRELERİ ÜZERİNE ETKİLERİ

Year 2021, , 275 - 282, 15.06.2021

Ali Serdar Oğuzoğlu , Nilgün Şenol , İlter İlhan , Halil Aşcı , Mine Kaynak , Selçuk Çömlekci

https://doi.org/10.17343/sdutfd.849527

Abstract

Amaç
Bu çalışmada, Subaraknoid Kanama (SAK) sonrasında
serebellar dokuda meydana gelen oksidatif stres
üzerinde, uygulanabilecek farklı tedavi modalitelerinin
etkilerinin değerlendirilmesi amaçlanmıştır.
Gereç-ve Yöntem
Kuyruk arterlerinden alınan 0,3 cc otolog kanın sisterna
magna bölgesine enjekte edilmesi ile SAK oluşturulan
ratlara farklı dozlarda (30-60 mg/kg Pregabalin
(PREG), Nimodipin (NİMO), Salubrinal (SLB) ve Darbeli
Elektromanyetik Alan (DEMA) uygulandı. Sakrifikasyon
sonrasında alınan serebellum dokularında
total oksidan kapasite (TOS), total antioksidan kapasite
(TAS) ve oksidatif stres indeksi (OSİ) gibi oksidatif
stres parametreleri incelendi.
Bulgular
TOS ve OSİ değerlerinde PREG30 (iki değer için de
p=0.003), PREG₆₀ (p=0.026 ve p=0.005, sırasıyla),
SLB ve DEMA uygulanan gruplarda anlamlı olarak
azalma görüldü. TAS seviyelerinde ise PREG ve
DEMA uygulanan gruplarda istatistiksel olarak anlamlı
bir değişiklik saptanmazken, SLB ve NİMO uygulanan
gruplarda anlamlı değişiklik olduğu görüldü.
NİMO uygulanan grupta OSİ değerinde de (p=0,046)
SAK grubuna göre anlamlı bir azalma saptandı.
Sonuç
PREG ve SLB SAK’ta farklı iki mekanizma üzerinden
koruyucu olabilmektedir. DEMA tedavisinin de etkinliğinden
söz edilebilir ancak etkisinin değerlendirilmesi
için farklı uygulama zamanları ve süreleri ile yeni çalışmalara
ihtiyaç vardır.

Keywords

subaraknoid kanama, oksidatif stres, pregabalin, DEMA, sıçan, serebellum

Supporting Institution

Yok

Project Number

Yok

Thanks

-

THE EFFECTS OF DIFFERENT TREATMENT MODALITIES ON OXIDATIVE STRESS MARKERS FOR CEREBELLAR INJURY SECONDARY TO SUBARACHNOID HEMORRHAGE

Abstract

Objective
In this study, we aimed to evaluate the effects of different
treatment modalities on oxidative stress occured in
cerebellar tissue after subarachnoid hemorrhage (SAH).
Materials and Methods
Different doses of Pregabaline (30-60 mg/kg Pregabalin
(PREG), and Nimodipine (NIMO), Salubrinal
(SLB), Pulsed ElectromagneticFfield (PEMF) was
used in the rats, that SAH was comprised via 0,3cc
otolog blood injection into the cisterna magna. After
sacrification oxidative stress parameters like Total Antioxidant
Status (TAS), Total Oxidant Status (TOS),
and Oxidative Stress Index (OSI) were evaluated in
the cerellum tissues.
Results
Significant decrease was seen in the TOS and OSI
values of the groups that PREG30 (p=0.003 for both),
PREG₆₀ (p=0.026 and p=0.005, respectively), SLB
and PEMF were used. Although there was no statistically
difference in the TAS values of the PREG and
DEMA groups, significant difference was seen in the
SLB and NIMO groups. In the NIMO group there was
a significant decrease (p=0,046) in the OSI values
according to the SAH group.
Conclusion
PREG and SLB can be protective in SAH via two different
mechanisms, PEMF treatment can be effective
but new studies with different application periods and
doses are needed to evaluate the effects.

Keywords

subarachnoid hemorrhage, oxidative stress, pregabaline, PEMF, rat, cerebellum

Project Number

Yok

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