Ultrastructural analysis of a globozoospermia case

Year 2007, Volume: 14 Issue: 1, 29 - 32, 30.04.2009

Meltem Özgüner Oya Evirgen

Abstract

SüleymanDemirel Üniversitesi
TIP FAKÜLTESİ DERGİSİ: 2007 Mart; 14(1)










Ultrastructural analysis of a globozoospermia case



Meltem Özgüner*, Oya Evirgen**



*Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

**Department of Histology and Embryology, Faculty of Medicine, Ankara University, Ankara, Turkey



Özet



Globozospermia olgusunun ultrastrüktürel analizi



Globozoospermi, infertiliteye sebep olabilen ve nadir görülen bir sperm bozukluğudur. Globozoospermiye ikincil uzun süreli infertilite sorunu olan bir çift değerlendirildi. Globozoosperminin ICSI yöntemiyle başarılı şekilde tedavisi sonrası Transmisyon Elektron Mikroskopik (TEM) inceleme yapıldı. İncelenen çiftin ilk ICSI denemesinde %50 fertilizasyon oranı elde edildi. İlk denemede, başarılı embriyo transferi sonrasında bir gebelik oluştu ancak düşükle sonlandı. İkinci denemede, ICSI işlemi uygulanan 7 oositin 3'ü fertilize oldu ve normal yarıklanma elde edildi. Spontan oosit aktivasyonu sonrası elde edilen bu embriyoların transferi sonrasında dişi fötüse sahip tekli gebelik oluştu ve gestasyonun 36.haftasında doğum gerçekleşti. Spermatozoanın geriye dönük incelenmesinde orta parça ve kuyruk defekti olmaksızın globozoosperminin morfolojik bulguları saptandı. Mevcut ICSI işlemleri ile globozoospermiye ikincil gelişen infertilite sorununun çözülebileceği sonucuna varıldı ve bu tip vakalarda globozoosperminin ultrastrüktürel düzeyde analizinin, sonuçların önceden tahmininde faydalı bir rehber olabileceği düşünüldü.



Anahtar kelimeler: Globozoospermi, ICSI, ultrastrüktürel analiz



Abstract



Globozoospermia is an uncommon sperm disorder which may lead to infertility. A couple with long term infertility secondary to globozoospermia was evaluated. The successful outcome of ICSI treatment associated with globozoospermia was evaluated with Transmission Electron Microscopy (TEM). The couple experienced 50% fertilization rate after ICSI at their first attempt. On the first cycle, successful embryo transfer and a pregnancy was achieved but resulted with abortion. On the second cycle, 3 of the 7 injected oocytes were fertilized after ICSI and normal cleavage was achieved. Transfer of these embryos obtained by spontaneous oocyte activation with globozoospermia resulted a singleton pregnancy with a female fetus and delivery occured after 36 weeks of gestation. Retrospective analysis of spermatozoa reflected the morphologic abnormalities of globozoospermia without mid-piece and tail defects. It is concluded that current ICSI procedures may overcome the infertility secondary to globozoospermia and ultrastructural analysis of globozoospermia seems to be a useful guide in order to predict the outcomes in such cases.



Key words: Globozoospermia, ICSI, ultrastructural analysis

Keywords

Globozoospermi, ICSI, ultrastrüktürel analiz

References

• Wolff HH, Schill WB, Moritz P. Round-headed spermatozoa: a rare andrologic finding (‘globe-headed spermatozoa’, ‘globozoospermia’). Hautartz 1976; 27(3): 111-6.
• Aitken RJ, Kerr L, Bolton V, Hargreave T. Analysis of sperm function in globozoospermia: implications for the mechanism of sperm-zona interaction. Fertil Steril 1990; 54: 701-7.
• Syms AJ, Johnson AR, Lipschultz LI, Smith RG. Studies on human spermatozoa with round-headed syndrome. Fertil Steril 1984; 42: 431-5.
• Dale B, Iaccarino M, Fortuna A,Gragnaniello G, Kyozuka K, Tosti E. A morphological and functional study of fusibility in round-headed spermatozoa in the human. Fertil Steril 1994; 61: 336-40.
• Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340: 17- 18.
• Bourne H, Liu DY, Clarke GN, Baker HW. Normal fertilization and embryo development by intracytoplasmic sperm injection of round headed acrosomeless sperm. Fertil Steril 1995; 63: 1329-32.
• Liu J, Nagy Z, Joris H, Tournaye H, Smitz J, Camus M, et al. Successful fertilization and establishment of pregnancies after intracytoplasmic injection in patients with globozoospermia. Hum Reprod 1995; 10: 626- 29.
• Trokoudes KM, Danos N, Kalogirou L, Vlachou R, Lysiotis T, Georghiades N, et al. Pregnancy with spermatozoa from a globozoospermic man after intracytoplasmic sperm injection treatment. Hum Reprod 1995; 10: 880-82.
• Edirisinghe WR, Murch AR, Junk SM, Yovich JL. Cytogenetic analysis of unfertilized oocytes following intracytoplasmic sperm injection using spermatozoa from globozoospermic man. Hum Reprod 1998; 13: 3094-98.
• Battaglia DE, Koehler JK, Klein NA, Tucker MJ. Failure of oocyte activation after intracytoplasmic sperm injection using round-headed sperm. Fertil Steril 1997; 68: 118-22.
• World Health Organization. WHO Laboratory Manual for the Examination of Human Semen. Cambridge, United Kingdom: Cambridge University Pres; 1992.
• Check JH, Kozak J, Katsoff D. Hypoosmotic Swelling: evaluation of sperm tails using strict criteria in cases of globozoospermia. Arch Androl 1993; 30: 125-28.
• Rybouchkin A, Dozortsev D, Pelinck MJ, De Sutter P,Dhont M. Analysis of the oocyte activating capacity and chromosomal complement of round-headed human spermatozoa by their injection into Mouse oocytes. Hum Reprod 1996; 11: 2170-75.
• Rybouchkin AV, Van der Straeten F, Quatacker J, De Sutter P, Dhont M. Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic injection in a case of round-headed sperm associated with deficient oocyte activation capacity. Frtil Steril 1997; 68: 1144-47.
• Stone S, O’Mahony F, Khalaf Y, Taylor A, Braude P. Anormal livebirth after intracytoplasmic sperm injection for globozoospoermia without assisted oocyte activation. Hum Reprod 2000; 15: 139-41.