Yıl 2020, Cilt 27 , Sayı 1, Sayfalar 105 - 112 2020-03-01

Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model

Alper GÜLTEKİN [1] , Meric UNAL [2] , Mehtat ÜNLÜ [3] , Safa SATOĞLU [4]


Purpose:Human amniotic fluid contains several growth factors and hyaluronic acid. It has positive effect in healing the tendon, nerve and bone injuries. Human amniotic membrane has positive effects on tendon, nerve healing and is also used for skin healing in burns and complex wounds. We hypothesized that human amniotic fluid and human amniotic membrane have positive stimulating effects on fracture healing.  The aim of this study is to investigate the effects of human amniotic fluid and human amniotic membrane on bone healing in rat tibial bone fracture model. 

Materials and Methods:A 72 open fracture model was created for both tibial bones of 36 male Wistar rats. They were divided into three groups. Group-1 was the control group. In Group-2, human amniotic fluid which was taken from amniocentesis of seronegative pregnants in their second trimester was injected to the fracture site. In group-3, human amniotic membrane which was taken from normal seronegative term pregnants after their caesarean sections was wrapped around fracture site and then human amniotic fluid was injected to the fracture site. Radiological fracture union patterns and histopathological evaluations with Modified Lane-Sandhu classification were performed with Modified Lane-Sandhu classification at the end of third and sixth weeks.

Results:At third week, Group-2 and Group-3 had better histopathological results than Group-1. At sixth week, Group-2 had significantly better results than Group-1. Group-3 had also better results than Group-1 although there was no statistical significance. At third and sixth weeks, histopathological scores of Group-2 were significantly better than Group-3(p=0.021, p=0.019).  

Conclusion:Our data suggested that human amniotic fluid had positive effect while human amniotic membrane had no positive effect on bone healing. With its low immunity and easy delivery, human amniotic membrane can also be used to stimulate bone healing in combination with human amniotic fluid with different application techniques.

Tibia fracture model, human amniotic fluid, human amniotic membrane, animal study
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Birincil Dil en
Konular Tıp
Bölüm Araştırma Makaleleri
Yazarlar

Yazar: Alper GÜLTEKİN (Sorumlu Yazar)
Kurum: SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, KOCAELİ DERİNCE SAĞLIK UYGULAMA VE ARAŞTIRMA MERKEZİ
Ülke: Turkey


Yazar: Meric UNAL
Kurum: FACULTY OF MEDICINE
Ülke: Turkey


Yazar: Mehtat ÜNLÜ
Kurum: DOKUZ EYLUL UNIVERSITY, FACULTY OF MEDICINE
Ülke: Turkey


Yazar: Safa SATOĞLU
Kurum: DOKUZ EYLUL UNIVERSITY, FACULTY OF MEDICINE

Tarihler

Başvuru Tarihi : 11 Şubat 2019
Kabul Tarihi : 15 Nisan 2019
Yayımlanma Tarihi : 1 Mart 2020

Bibtex @araştırma makalesi { sdutfd525766, journal = {SDÜ Tıp Fakültesi Dergisi}, issn = {1300-7416}, eissn = {2602-2109}, address = {}, publisher = {Süleyman Demirel Üniversitesi}, year = {2020}, volume = {27}, pages = {105 - 112}, doi = {10.17343/sdutfd.525766}, title = {Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model}, key = {cite}, author = {GÜLTEKİN, Alper and UNAL, Meric and ÜNLÜ, Mehtat and SATOĞLU, Safa} }
APA GÜLTEKİN, A , UNAL, M , ÜNLÜ, M , SATOĞLU, S . (2020). Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model. SDÜ Tıp Fakültesi Dergisi , 27 (1) , 105-112 . DOI: 10.17343/sdutfd.525766
MLA GÜLTEKİN, A , UNAL, M , ÜNLÜ, M , SATOĞLU, S . "Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model". SDÜ Tıp Fakültesi Dergisi 27 (2020 ): 105-112 <https://dergipark.org.tr/tr/pub/sdutfd/issue/52788/525766>
Chicago GÜLTEKİN, A , UNAL, M , ÜNLÜ, M , SATOĞLU, S . "Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model". SDÜ Tıp Fakültesi Dergisi 27 (2020 ): 105-112
RIS TY - JOUR T1 - Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model AU - Alper GÜLTEKİN , Meric UNAL , Mehtat ÜNLÜ , Safa SATOĞLU Y1 - 2020 PY - 2020 N1 - doi: 10.17343/sdutfd.525766 DO - 10.17343/sdutfd.525766 T2 - SDÜ Tıp Fakültesi Dergisi JF - Journal JO - JOR SP - 105 EP - 112 VL - 27 IS - 1 SN - 1300-7416-2602-2109 M3 - doi: 10.17343/sdutfd.525766 UR - https://doi.org/10.17343/sdutfd.525766 Y2 - 2019 ER -
EndNote %0 SDÜ Tıp Fakültesi Dergisi Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model %A Alper GÜLTEKİN , Meric UNAL , Mehtat ÜNLÜ , Safa SATOĞLU %T Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model %D 2020 %J SDÜ Tıp Fakültesi Dergisi %P 1300-7416-2602-2109 %V 27 %N 1 %R doi: 10.17343/sdutfd.525766 %U 10.17343/sdutfd.525766
ISNAD GÜLTEKİN, Alper , UNAL, Meric , ÜNLÜ, Mehtat , SATOĞLU, Safa . "Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model". SDÜ Tıp Fakültesi Dergisi 27 / 1 (Mart 2020): 105-112 . https://doi.org/10.17343/sdutfd.525766
AMA GÜLTEKİN A , UNAL M , ÜNLÜ M , SATOĞLU S . Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model. SDÜ Tıp Fakültesi Dergisi. 2020; 27(1): 105-112.
Vancouver GÜLTEKİN A , UNAL M , ÜNLÜ M , SATOĞLU S . Effect of Human Amniotic Fluid and Membrane on Fracture Healing on Rat Fracture Model. SDÜ Tıp Fakültesi Dergisi. 2020; 27(1): 112-105.