IN SILICO ANALYSIS OF THE POTENTIAL ROLE OF SRY GENE IN PARKINSON’S DISEASE

Yıl 2023, Cilt: 30 Sayı: 1, 19 - 24, 14.03.2023

Muhammet Ay

https://doi.org/10.17343/sdutfd.1166918

Öz

Objective
Parkinson’s disease (PD) is the most common movement
disorder and is caused by the loss of dopaminergic
neurons in the substantia nigra. The first
motor symptoms of PD begin to appear when more
than 60% of dopaminergic neurons are already lost.
Although the etiology of PD is not fully understood,
PD is believed to result from the complex interactions
between genetic and environmental factors. Previous
studies demonstrated that men are at least 1.5 times
more likely to develop PD than women. The current
kulstudy
aimed to investigate whether SRY, a chromosome
gene, plays a role in the pathogenesis of PD
using in silico methods.
Material and Method
The mRNA expression of SRY in different human tissues
was analyzed using the Human Protein Atlas
(HPA). The interactions between SRY and PD-related
genes were analyzed using the STRING and
GeneMANIA databases. The JASPAR database was
used to determine the putative binding sites of SRY in
the promoter regions of some PD-related genes. Finally,
the BioGRID and GeneMANIA databases were
used to identify the key genes that interact with SRY.
Results
In silico analyses revealed that there are SRY binding
sites in the promoter regions of some PD-associated
genes and that SRY can regulate androgen receptor
(AR) signaling.
Conclusion
These findings suggest that SRY may play a role in
the pathogenesis of PD and may thus serve as a molecular
target for developing new therapeutic strategies
against PD.

Anahtar Kelimeler

Parkinson’s disease, SRY, expression, promoter, in silico

SRY GENİNİN PARKİNSON HASTALIĞINDAKİ POTANSİYEL ROLÜNÜN İN SİLİKO ANALİZİ

Öz

Amaç
Parkinson hastalığı (PH) en sık görülen hareket hastalığıdır
ve substansiya nigra bölgesindeki dopaminerjik
nöronların kaybından kaynaklanmaktadır. PH’nin ilk
motor semptomları ortaya çıkmaya başladığında dopaminerjik
nöronların %60’ından fazlası zaten ölmüştür.
PH’nin etiyolojisi tam olarak anlaşılmamış olsa
da, PH’nin genetik ve çevresel faktörler arasındaki
kompleks etkileşimler sonucu oluştuğuna inanılmaktadır.
Önceki çalışmalar erkeklerin PH’ye yakalanma
olasılığının kadınlara göre en az 1,5 kat daha fazla
olduğunu göstermiştir. Bu çalışma, bir Y kromozomu
geni olan SRY’nin PH’nin patogenezinde bir rol oynayıp
oynamadığını in siliko yöntemlerle araştırmayı
amaçlamıştır.
Gereç ve Yöntem
Farklı insan dokularındaki SRY mRNA ekspresyonu
İnsan Protein Atlas (HPA)’yı kullanarak analiz edilmiştir.
SRY ve PH ile ilişkili genler arasındaki etkileşimler
STRING ve GeneMANIA veri tabanlarını kullanarak
incelenmiştir. JASPAR veri tabanı, PH ile ilişkili bazı
genlerin promotör bölgelerindeki muhtemel SRY bağlanma
noktalarını belirlemek için kullanılmıştır. Son
olarak, SRY ile etkileşim halindeki anahtar genleri belirlemek
için BioGRID ve GeneMANIA veri tabanları
kullanılmıştır.
Bulgular
İn siliko analizler PH ile ilişkili bazı genlerin promotör
bölgelerinde SRY bağlanma noktası olduğunu ve SRY’nin
androjen reseptör (AR) sinyalini düzenleyebileceğini
ortaya çıkarmıştır.
Sonuç
Bu bulgular SRY’nin PH’nin patogenezinde rol oynayabileceğini
ve bundan dolayı da PH’ye karşı yeni terapötik
stratejiler geliştirmek için moleküler bir hedef
vazifesi görebileceğini önermektedir.

Anahtar Kelimeler

Parkinson hastalığı, SRY, ekspresyon, promotör, in siliko

Kaynakça

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