Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm

Tarik Ahmet; Öznur Küçük Keleş; Mukadder İnci Başer Kolcu; Ezgi Şababoğlu Baytaroğlu; Sema Belli

doi:10.15311/selcukdentj.1744561

Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm

Abstract

Background: To evaluate the effects of hydrogen peroxide(H2O2) and boric-acid(BA) on E. faecalis biofilm with or without photo-activation by Germinator (Design for Vision, USA). Materials: Biofilm formation was provided on sterilized dentin discs. Five groups were created(n=8): 1.5%, 3% and 5% H2O2; 5 and 10% BA. Each was exposed to irrigation-solutions with or without photo-activation. ‘Biofilm killing-percentage’ (100%), (CFU/ml) was calculated, colony-counting was performed. One-way analysis of variance, Duncan, Tukey's tests were provided. Results: All irrigants reduced number of viable-bacteria in biofilm (p<0.05). H2O2with/without photo-activation 100% increased the effect on biofilm. 10%BA with or without photo-activation was found more effective than 5%BA concentration (p<0.001). Effects of H2O2 with or without photo-activation on biofilm was higher than BA groups (p<0.001). Conclusions: H2O2 with or without photo-activation is an effective solution against E.faecalis biofilm. 5 and 10%BA seem promising against E. faecalis while 10% seems more effective regardless the effect of photo-activation. Keywords: Germinator, Biofilm, Boric acid, Hydrogen peroxide, Chlorhexidine

Keywords

Supporting Institution

Scientific Research Projects Coordination Center (BAP) of Selçuk University (21212046).

Ethical Statement

This study is ethically approved by Non-Interventional Clinical Research Evaluation Commission, Faculty of Dentistry, Selcuk University with the decision number of 2020/61.

Thanks

We are grateful to Prof. Dr. Liviu Steier and John Walsh for their kind support about having Germinator Device and for their patience about receiving the results during the Covid 19 Pandemic. We thank Designs for Vision Company for their generous equipment support.

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Details

Primary Language

English

Subjects

Endodontics

Journal Section

Research Article

Authors

Tarik Ahmet
0000-0002-5040-7234
Türkiye

Öznur Küçük Keleş ^*
0000-0002-7303-0601
Türkiye

Mukadder İnci Başer Kolcu
0000-0002-2996-7632
Türkiye

Ezgi Şababoğlu Baytaroğlu
0000-0003-3566-5443
Türkiye

Sema Belli
0000-0002-7240-7649
Türkiye

Publication Date

April 27, 2026

Submission Date

July 18, 2025

Acceptance Date

September 15, 2025

Published in Issue

Year 2026 Volume: 13 Number: 1

DOI

https://doi.org/10.15311/selcukdentj.1744561

IZ

https://izlik.org/JA38KC67HT

Cite

RIS / Bibtex

APA

Ahmet, T., Küçük Keleş, Ö., Başer Kolcu, M. İ., Şababoğlu Baytaroğlu, E., & Belli, S. (2026). Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm. Selcuk Dental Journal, 13(1), 79-84. https://doi.org/10.15311/selcukdentj.1744561

AMA

1.Ahmet T, Küçük Keleş Ö, Başer Kolcu Mİ, Şababoğlu Baytaroğlu E, Belli S. Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm. Selcuk Dent J. 2026;13(1):79-84. doi:10.15311/selcukdentj.1744561

Chicago

Ahmet, Tarik, Öznur Küçük Keleş, Mukadder İnci Başer Kolcu, Ezgi Şababoğlu Baytaroğlu, and Sema Belli. 2026. “Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm”. Selcuk Dental Journal 13 (1): 79-84. https://doi.org/10.15311/selcukdentj.1744561.

EndNote

Ahmet T, Küçük Keleş Ö, Başer Kolcu Mİ, Şababoğlu Baytaroğlu E, Belli S (April 1, 2026) Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm. Selcuk Dental Journal 13 1 79–84.

IEEE

[1]T. Ahmet, Ö. Küçük Keleş, M. İ. Başer Kolcu, E. Şababoğlu Baytaroğlu, and S. Belli, “Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm”, Selcuk Dent J, vol. 13, no. 1, pp. 79–84, Apr. 2026, doi: 10.15311/selcukdentj.1744561.

ISNAD

Ahmet, Tarik - Küçük Keleş, Öznur - Başer Kolcu, Mukadder İnci - Şababoğlu Baytaroğlu, Ezgi - Belli, Sema. “Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm”. Selcuk Dental Journal 13/1 (April 1, 2026): 79-84. https://doi.org/10.15311/selcukdentj.1744561.

JAMA

1.Ahmet T, Küçük Keleş Ö, Başer Kolcu Mİ, Şababoğlu Baytaroğlu E, Belli S. Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm. Selcuk Dent J. 2026;13:79–84.

MLA

Ahmet, Tarik, et al. “Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm”. Selcuk Dental Journal, vol. 13, no. 1, Apr. 2026, pp. 79-84, doi:10.15311/selcukdentj.1744561.

Vancouver

1.Tarik Ahmet, Öznur Küçük Keleş, Mukadder İnci Başer Kolcu, Ezgi Şababoğlu Baytaroğlu, Sema Belli. Do Concentration or Photo-Activation Change Effect of Hydrogen Peroxide And Boric Acid On E.Faecalis Biofilm. Selcuk Dent J. 2026 Apr. 1;13(1):79-84. doi:10.15311/selcukdentj.1744561