Does hypochlorous-acid have an effect on dissolving bovine pulp tissue?

Durmuş Alperen Bozkurt; Arslan Terlemez; Duygu Bozkurt

doi:10.15311/selcukdentj.1810336

Does hypochlorous-acid have an effect on dissolving bovine pulp tissue?

Abstract

Background/purpose: The aim of the study is to evaluate whether hypochlorous acid (HOCl) has an effect on the dissolution of bovine pulp tissue. Materials and methods: Sixty bovine-pulp-tissue samples were randomly divided into six experimental groups (n=10): Group I- 5.25% sodium hypochlorite (NaOCl), Group II- 2.6% NaOCl, Group III- 1.3% NaOCl, Group IV- HOCl, Group V- 17% Ethylenediaminetetraaceticacid (EDTA), Group VI- Distilled-Water. Pulp samples were immersed in coded solutions in ten minute intervals for the first three-hours, after that they were removed from the solution in sixty-minute intervals. The difference in the weight of the tissue sample was measured before and after the exposure to the test solution, until no weight could be measured with the remaining existing tissue up to a total of one week. The time required for the solutions to dissolve the tissue (min) divided by the initial weight and tissue loss per-minute calculated as mg/min. The data were statistically analyzed using the Kruskal–Wallis and Mann–Whitney U-tests (α=0.05). Results: Showed that a significant tissue dissolution in the chlorine-containing groups (HOCl and NaOCl), while no significant tissue dissolution occurred in distilled water and 17% EDTA groups. There was a significant difference between HOCl and NaOCl solutions (P < 0.001). Conclusion: The results of this study confirmed that eventhough the tissue dissolution rate of HOCl was less than NaOCl, it could completely dissolve the pulp tissue.

Keywords

Supporting Institution

Necmettin Erbakan University Scientific Research Project Coordination Department

Project Number

221224002

Ethical Statement

Ethical approwal was held from Necmettin Erbakan University Ethics Committe for non-pharmasotical and non-medical products research bye the decision number of 2021/05-59

Thanks

Thanks to Necmettin Erbakan University Scientific Research Project Coordination Department for funding this research

References

1. Moorer WR, Wesselink PR. Factors promoting the tissue dissolving capability of sodium hypochlorite. Int Endod J 1982; 15:187-96.
2. Tsotsis P, Dunlap C, Scott R, Arias A, Peters OA. A survey of current trends in root canal treatment: access cavity design and cleaning and shaping practices. Aust Endod J 2020; 47:27-33.
3. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clin Microbiol Rev 1999; 12:147-79.
4. Rossi-Fedele G, Guastalli AR, Dogramaci EJ, Steier L, De Figueiredo JA. Influence of pH changes on chlorine-containing endodontic irrigating solutions. Int Endod J 2011; 44:792-9.
5. Mainnemare A, Megarbane B, Soueidan A, Daniel A, Chapple IL. Hypochlorous acid and Taurine-N-monochloramine in periodontal diseases. J Dent Res 2004; 83:823-31.
6. Kettle AJ, Winterbourn CC. Myeloperoxidase: a key regulator of neutrophil oxidant production. Redox Rep 1997; 3:3-15.
7. Lapenna D, Cuccurullo F. Hypochlorous acid and its pharmacological antagonism: an update picture. Gen Pharmacol 1996; 27:1145-7.
8. Wang L, Bassiri M, Najafi R, et al. Hypochlorous acid as a potential wound care agent: part I. Stabilized hypochlorous acid: a component of the inorganic armamentarium of innate immunity. J Burns Wounds 2007;6: e5.

9. Block MS, Rowan BG. Hypochlorous Acid: A Review. J Oral Maxillofac Surg 2020; 78:1461-6.
10. Aubut V, Pommel L, Verhille B, et al. Biological properties of a neutralized 2.5% sodium hypochlorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109: e120-5.
11. Camps J, Pommel L, Aubut V, et al. Shelf life, dissolving action, and antibacterial activity of a neutralized 2.5% sodium hypochlorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108: e66-73.
12. Christensen CE, McNeal SF, Eleazer P. Effect of lowering the pH of sodium hypochlorite on dissolving tissue in vitro. J Endod 2008; 34:449-52.
13. Macedo RG, Wesselink PR, Zaccheo F, Fanali D, Van Der Sluis LW. Reaction rate of NaOCl in contact with bovine dentine: effect of activation, exposure time, concentration and pH. Int Endod J 2010; 43:1108-15.
14. Clarkson RM, Moule AJ, Podlich H, et al. Dissolution of porcine incisor pulps in sodium hypochlorite solutions of varying compositions and concentrations. Aust Dent J 2006; 51:245-51.
15. Stojicic S, Zivkovic S, Qian W, Zhang H, Haapasalo M. Tissue dissolution by sodium hypochlorite: effect of concentration, temperature, agitation, and surfactant. J Endod 2010; 36:1558-62.
16. Hand RE, Smith ML, Harrison JW. Analysis of the effect of dilution on the necrotic tissue dissolution property of sodium hypochlorite. J Endod 1978; 4:60-4.
17. Trautmann E, Attin T, Mohn D, Zehnder M. Hydrogen Peroxide Versus Sodium Hypochlorite: All a Matter of pH? J Endod 2021; 47:297-302.
18. Okino LA, Siqueira EL, Santos M, Bombana AC, Figueiredo JA. Dissolution of pulp tissue by aqueous solution of chlorhexidine digluconate and chlorhexidine digluconate gel. Int Endod J 2004; 37:38-41.
19. Naenni N, Thoma K, Zehnder M. Soft tissue dissolution capacity of currently used and potential endodontic irrigants. J Endod 2004; 30:785-7.
20. Zehnder M, Kosicki D, Luder H, Sener B, Waltimo T. Tissue-dissolving capacity and antibacterial effect of buffered and unbuffered hypochlorite solutions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002; 94:756-62.
21. de Almeida LH, Leonardo NG, Gomes AP, Giardino L, Souza EM, Pappen FG. Pulp tissue dissolution capacity of sodium hypochlorite combined with cetrimide and polypropylene glycol. Braz Dent J 2013; 24:477-81.
22. Aytekin K, Duman A, Aytekin FY, et al. Evaluation of Necrotic, Edematous and Inflammatory Changes in Bone Marrow and Soft Tissue After Irrigation with Different Concentrations of NaOCl Solution: An Experimental Study in Rat Tibia. Eurasian J Med 2020; 52:243-8.
23. Zehnder M. Root canal irrigants. J Endod 2006; 32:389-98.
24. Heling I, Rotstein I, Dinur T, Szwec-Levine Y, Steinberg D. Bactericidal and cytotoxic effects of sodium hypochlorite and sodium dichloroisocyanurate solutions in vitro. J Endod 2001;27:278-80.
25. Beltz RE, Torabinejad M, Pouresmail M. Quantitative analysis of the solubilizing action of MTAD, sodium hypochlorite, and EDTA on bovine pulp and dentin. J Endod 2003; 29:334-7.
26. Ballal NV, Mala K, Bhat KS. Effect of maleic acid and ethylenediaminetetraacetic acid on the dissolution of human pulp tissue--an in vitro study. Int Endod J 2011; 44:353-6.

Details

Primary Language

English

Subjects

Endodontics

Journal Section

Research Article

Authors

Durmuş Alperen Bozkurt
0000-0003-3910-5777
Türkiye

Arslan Terlemez ^*
0000-0002-6092-4817
Türkiye

Duygu Bozkurt
0000-0003-2178-7829
Türkiye

Publication Date

December 29, 2025

Submission Date

October 25, 2025

Acceptance Date

December 25, 2025

Published in Issue

Year 2025 Volume: 12 Number: 3

DOI

https://doi.org/10.15311/selcukdentj.1810336

IZ

https://izlik.org/JA74UX77MX

Cite

RIS / Bibtex

APA

Bozkurt, D. A., Terlemez, A., & Bozkurt, D. (2025). Does hypochlorous-acid have an effect on dissolving bovine pulp tissue? Selcuk Dental Journal, 12(3), 461-465. https://doi.org/10.15311/selcukdentj.1810336

AMA

1.Bozkurt DA, Terlemez A, Bozkurt D. Does hypochlorous-acid have an effect on dissolving bovine pulp tissue? Selcuk Dent J. 2025;12(3):461-465. doi:10.15311/selcukdentj.1810336

Chicago

Bozkurt, Durmuş Alperen, Arslan Terlemez, and Duygu Bozkurt. 2025. “Does Hypochlorous-Acid Have an Effect on Dissolving Bovine Pulp Tissue?”. Selcuk Dental Journal 12 (3): 461-65. https://doi.org/10.15311/selcukdentj.1810336.

EndNote

Bozkurt DA, Terlemez A, Bozkurt D (December 1, 2025) Does hypochlorous-acid have an effect on dissolving bovine pulp tissue? Selcuk Dental Journal 12 3 461–465.

IEEE

[1]D. A. Bozkurt, A. Terlemez, and D. Bozkurt, “Does hypochlorous-acid have an effect on dissolving bovine pulp tissue?”, Selcuk Dent J, vol. 12, no. 3, pp. 461–465, Dec. 2025, doi: 10.15311/selcukdentj.1810336.

ISNAD

Bozkurt, Durmuş Alperen - Terlemez, Arslan - Bozkurt, Duygu. “Does Hypochlorous-Acid Have an Effect on Dissolving Bovine Pulp Tissue?”. Selcuk Dental Journal 12/3 (December 1, 2025): 461-465. https://doi.org/10.15311/selcukdentj.1810336.

JAMA

1.Bozkurt DA, Terlemez A, Bozkurt D. Does hypochlorous-acid have an effect on dissolving bovine pulp tissue? Selcuk Dent J. 2025;12:461–465.

MLA

Bozkurt, Durmuş Alperen, et al. “Does Hypochlorous-Acid Have an Effect on Dissolving Bovine Pulp Tissue?”. Selcuk Dental Journal, vol. 12, no. 3, Dec. 2025, pp. 461-5, doi:10.15311/selcukdentj.1810336.

Vancouver

1.Durmuş Alperen Bozkurt, Arslan Terlemez, Duygu Bozkurt. Does hypochlorous-acid have an effect on dissolving bovine pulp tissue? Selcuk Dent J. 2025 Dec. 1;12(3):461-5. doi:10.15311/selcukdentj.1810336