Evaluation of Total Oxidant and Antioxidant Status of Various Pulp Capping Materials on Human Dental Pulp Stem Cells

Abstract

Background: The aim of this study was to evaluate the total oxidant and antioxidant status of five pulp capping materials (Mineral Trioxide Aggregate, Biodentine, Pulpine Mineral, Endo Repair and Zinc Oxide Eugenol) on human dental pulp stem cells.

Methods: In our study, American Type Culture Collection human dental pulp stem cells were used. The filter diffusion test was applied according to ISO standards. At the end of 24^th and 72^nd hours, biochemical analysis was conducted (n=3). Kruskal Wallis Variance Analysis and Mann-Whitney U test were used for independent group comparisons between measurements.

Results: As a result of 24 hours evaluation, only Mineral Trioxide Aggregate exhibits antioxidative protection similar to control group (p> 0.05), while other groups remain below this level. It was determined that the total antioxidant status of all groups was increased between 24 and 72 hours (p<0.05).  At the end of 72nd hours, the best preservative groups were Biodentine and Pulpine Mineral, additionally the weakest protective group was Zinc Oxide Eugenol (p<0.05).            

Conclusions: According to the analysis, it was determined that all pulp capping materials have an oxidative effect on human dental pulp stem cells and also have an antioxidant defense mechanism. The highest protection in the acute exposure was found in the Mineral Trioxide Aggregate group and additionally, Biodentine and Pulpine Mineral (includig propolis) groups were found to be the most protective for a long time. The antioxidative effect of ZnOEu group was found to be insufficient. The propolis containing pulp capping material has been shown to be a good alternative to calcium phosphate products due to its positive effect on stem cells.

Keywords: Dental pulp capping, calcium phosphate, free radicals, propolis.

Keywords

• Dental pulp capping
• calcium phosphate
• free radicals
• propolis

Farklı Pulpa Kaplama Materyallerinin Toplam Oksidan ve Antioksidan Kapasitelerinin İnsan Dental Pulpa Kök Hücreleri Üzerinde Değerlendirilmesi

Abstract

Amaç: Bu çalışmanın amacı, beş farklı pulpa kaplama materyalinin (Mineral Trioksit Agregat, Biodentin, Pulpine Mineral, Endo Repair ve Çinko Oksit Öjenol) toplam oksidan ve antioksidan seviyelerinin insan dental pulpa kök hücreleri üzerinde değerlendirilmesidir.

Gereç ve Yöntemler:Çalışmamızda Amerikan Tipi Kültür Koleksiyonu insan dental pulpa kök hücreleri kullanılmıştır. Filtre difüzyon testi, ISO standartlarına göre uygulanmıştır. 24. ve 72. saatlerin sonunda biyokimyasal analiz yapılmıştır(n = 3). Ölçümler arasında bağımsız grup karşılaştırmaları için Kruskal Wallis Varyans Analizi ve Mann-Whitney U testi kullanılmıştır.

Bulgular: 24 saatlik değerlendirme sonucunda, sadece Mineral Trioksit Agregat, kontrol grubuna benzer antioksidatif koruma gösterirken (p> 0.05), diğer gruplar bu seviyenin altında kalmıştır. Tüm grupların toplam antioksidan durumunun 24 ve 72 saat arasında arttığı belirlenmiştir (p <0.05). 72 saatin sonunda, en iyi koruma Biodentin ve Pulpine Mineral gruplarındai en zayıf koruma ise Çinko Oksit Eugenol grubunda bulunmuştur (p <0.05).

Sonuçlar: Yapılan analize göre, tüm pulpa kaplama materyallerinin insan dental pulpa kök hücreleri üzerinde hem oksidatif etki hem de antioksidan savunma mekanizmasını uyarıcı etkiye sahip olduğu belirlenmiştir. İlk 24 saatte en yüksek korunma Mineral Trioxide Aggregate grubunda, 72 saatte ise; Biodentin ve propolis içeren Pulpine Mineral gruplarında bulunmuştur. ZnOEu grubunun antioksidatif etkisinin yetersiz olduğu tespit edilmiştir. Propolis içerikli pulpa kaplama materyalinin kök hücreler üzerindeki olumlu etkisi nedeniyle kalsiyum fosfat içerikli ürünlere iyi bir alternatif olabileceği ortaya konulmuştur.

Anahtar Kelimeler: Diş pulpa kaplaması, kalsiyum fosfat, serbest radikaller, propolis.

Keywords

• Diş pulpa kaplaması
• kalsiyum fosfat
• serbest radikaller
• propolis

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