Comparison Of Antibacterial Effects Of Pulp Capping Materials

Abstract

The aim of this study is to evaluate the antibacterial effects of six different pulp capping materials on Streptococcus mutans, Lactobacillus acidophilus and Enterococcus faecalis bacteria in different time periods. In our study, the antibacterial effects of TheraCal LC, Dycal, Biodentine, Calcimol LC, Activa and MTA Angelus were tested against strains of S. mutans, L. acidophilus and E. faecalis using the disk diffusion method. Each of the materials was hardened in standard, 5 mm diameter, hollow sterile discs prepared beforehand in line with the manufacturer's recommendations. The materials placed in the sample containers were disinfected under UV light device for 20 minutes. The materials were placed on petri dishes where microorganisms were cultivated at 2.5-3 cm intervals. The inhibition zone diameters around the discs that were left to incubate for 24-48 hours at 37°C were measured in millimeters. Statistical analysis of the data was performed using One Way ANOVA. At the end of the study, the tested materials showed a statistically significant antibacterial effect on all bacterial strains used (p<0.05). MTA Angelus material showed the highest effect on all three bacterial groups compared to other pulp capping materials. Calsimol LC agent had the greatest effect on L. acidophilus, while other agents used in the study showed the greatest effect on S. mutans. The antibacterial effect of all pulp capping materials used in the study was found to be higher at the end of the 48th hour than at the end of the 24th hour, but there was no statistically significant difference (p> 0.05).

Keywords

• Pulpa Kapak Ajanları
• Antibakteriyel Etki
• Disk Difüzyonu

Pulpa Kapak Malzemelerinin Antibakteriyel Etkilerinin Karşılaştırılması

Abstract

Bu çalışmanın amacı, altı farklı pulpa kapak materyalinin farklı zaman dilimlerinde Streptococcus mutans, Lactobacillus acidophilus ve Enterococcus faecalis bakterileri üzerindeki antibakteriyel etkilerini değerlendirmektir. Çalışmamızda TheraCal LC, Dycal, Biodentine, Calcimol LC, Activa ve MTA Angelus'un antibakteriyel etkileri disk difüzyon yöntemi kullanılarak S. mutans, L. acidophilus ve E. faecalis suşlarına karşı test edilmiştir. Malzemelerin her biri, üreticinin önerileri doğrultusunda önceden hazırlanmış standart, 5 mm çapında içi boş steril disklerde sertleştirildi. Numune kaplarına yerleştirilen malzemeler UV ışık cihazı altında 20 dakika süreyle dezenfekte edildi. Materyaller, 2.5-3 cm aralıklarla mikroorganizmaların yetiştirildiği petri kaplarına yerleştirildi. 37 ° C'de 24-48 saat inkübasyona bırakılan disklerin etrafındaki inhibisyon zonu çapları milimetre cinsinden ölçüldü. Verilerin istatistiksel analizi One Way ANOVA kullanılarak yapıldı. Çalışmanın sonunda, test edilen materyaller, kullanılan tüm bakteri suşları üzerinde istatistiksel olarak anlamlı bir antibakteriyel etki gösterdi (p <0.05). MTA Angelus materyali, diğer hamur kapama materyallerine kıyasla her üç bakteri grubu üzerinde en yüksek etkiyi göstermiştir. Calsimol LC ajanı, L. acidophilus üzerinde en büyük etkiye sahipken, çalışmada kullanılan diğer ajanlar S. mutans üzerinde en büyük etkiyi göstermiştir. Çalışmada kullanılan tüm pulpa kapak materyallerinin antibakteriyel etkisi 48. saat sonunda 24. saatin sonuna göre daha yüksek bulundu ancak istatistiksel olarak anlamlı bir fark yoktu (p> 0.05).

Keywords

• Pulp Capping Agents
• Antibacterial Effect
• Disk Diffusion

Supporting Institution

Yok

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