Pulpa Kuafajı ve Kuafaj Materyallerine Güncel Bir Bakış: Derleme

Yıl 2022, , 617 - 640, 24.08.2022

Dilek Akın , Çiğdem Atalayın Özkaya

https://doi.org/10.15311/selcukdentj.943113

Öz

Pulpa kuafajı; farklı materyaller yardımıyla çeşitli biyolojik süreçlerin aktive edildiği ve bu sayede tamir ve rejenerasyonun hedeflendiği oldukça önemli bir tedavi seçeneğidir. Pulpa kuafajında amaç; pulpanın canlılığını, fonksiyonlarını ve pulpa-dentin kompleksini korumaktır. Bu amaç doğrultusunda hedeflenen, ekspoze pulpa dokusu üzerine bir örtüleyici uygulayarak dentin benzeri yapı veya dentin köprüsü oluşumunu sağlamaktır. Kuafaj uygulamasının başarısını etkileyen pek çok faktör mevcuttur. Bu faktörler; hastanın yaşı, cinsiyeti, kuafaj uygulanacak diş ve konumu, pulpa ekspozunun nedeni, pulpa ekspozisyonun boyutu ve lokalizasyonu, kullanılan kuafaj materyali ve uygulanan restorasyon olarak özetlenebilir. Pulpa kuafajının başarılı olması için en önemli kritik faktörlerden biri; pulpa canlılığını ve fonksiyonunu koruyacak, doku ile uyumlu bir materyalin kullanılmasıdır. Günümüze kadar pulpa kuafajı için pek çok materyal incelenmiş ve tavsiye edilmiştir. Kuafaj amacıyla kullanılan materyaller; kalsiyum hidroksit içerikli materyaller, trikalsiyum silikat içerikli materyaller, rezin içerikli materyaller ve diğer potansiyel kuafaj materyalleri başlıkları altında sınıflandırılabilir. Mevcut materyaller birbirleriyle kıyaslandığında, çeşitli avantaj ve dezavantajlarının bulunduğu görülmektedir. Mevcut kuafaj materyallerinin dezavantajlarını ortadan kaldırmak, kuafaj uygulamalarında başarı oranını arttırabilmek ve ideal kuafaj materyaline ulaşabilmek için araştırmalar devam etmektedir. Bu doğrultuda mevcut materyallerde modifikasyonlar yapılmakta ve yeni potansiyel materyaller araştırılmaktadır.
Bu derlemenin amacı; geçmişten günümüze kadar kullanılan kuafaj materyallerinin içerikleri, etki mekanizmaları, avantaj-dezavantajları ve başarı oranları ile ilgili bilgileri ve araştırma bulgularını literatür doğrultusunda aktarmaktır.

Anahtar Kelimeler

Pulpa kuafajı, kuafaj materyalleri, kalsiyum hidroksit, trikalsiyum silikat

Kaynakça

• 1. Prof. Dr. Turan Cengiz, Endodonti, İzmir, Barış yayınları, 1990: 222.
• 2. Carlo Prati, Maria Giovanna Gandolfi.Calcium silicate bioactive cements: Biological perspectives and clinical applications. Dent Materials 2015; 31(4): 351-70.
• 3. Komabayashi, T.; Zhu, Q.; Eberhart, R.; Imai, Y. Current status of direct pulp-capping materials for permanent teeth. Dent. Mater. J. 2016, 35, 1–12.
• 4. Hermann B. Dentinobliteration der Wurzelkanäle nach Behandlung mit Calzium. Zahnärztl Rundschau 1930; 39: 888-898.
• 5. Cavalcanti BN, Rode SM, Marques MM. Cytotoxicity of substances leached or dissolved from pulp capping materials. Int Endod J 2005; 38: 505-509.
• 6. Marta Kunert, Monika Lukomska-Szymanska. Bio-Inductive Materials in Direct and Indirect Pulp Capping—A Review Article. Materials 2020; 13(5): 1204.
• 7. Cox, C.F.; Sübay, R.K.; Ostro, E.; Suzuki, S.; Suzuki, S.H. Tunnel defects in dentin bridges: Their formation following direct pulp capping. Oper. Dent. 1996, 21, 4–11.
• 8. Hilton, T.J. Keys to Clinical Success with Pulp Capping: A Review of the Literature. Oper. Dent. 2009; 34, 615–625.
• 9. Dougherty E, inventor. Dental cement material patent United States Patent & Trademark Office 3,047,408. 1962.
• 10. Sawusch R. Dycal capping of exposed pulps in primary teeth. J Dent Child 1963; 30: 141-149.
• 11. Simon, S.; Smith, A.J.; Lumley, P.J.; Cooper, P.R.; Berdal, A. The pulp healing process: From generation to regeneration. Endod. Top. 2012; 26, 41–56.
• 12. Hargreaves, K.M.; Goodis, H.E.; Seltzer, S. Seltzer and Bender’s Dental Pulp; Quintessence Pub. Co.: Chicago, IL, USA, 2002; ISBN 9780867154153.
• 13. Tewari S. Assessment of coronal microleakage in intermediately restored endodontic access cavities. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002; 93: 716-719.
• 14. Watts A, Paterson RC. The response of the mechanically exposed pulp to prednisolone and triamcinolone acetonide. Int Endod J 1988; 21: 9-16.
• 15. Goldberg M, Farges JC, Lacerda-Pinheiro S, Six N, Jegat N, Decup F, et al. Inflammatory and immunological aspects of dental pulp repair. Pharmacol Res 2008; 58: 137-147.
• 16. Goldberg M, Smith AJ. Cells and extracellular matrices of dentin and pulp: A biological basis for repair and tissue engineering. Crit Rev Oral Biol Med 2004; 15: 13-27.
• 17. Decup F, Six N, Palmier B, Buch D, Lasfargues JJ, Salih E, Goldberg M. Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat’s molar. Clin Oral Investig 2000; 4: 110-119.
• 18. Six N, Septier D, Chaussain-Miller C, Blacher R, DenBesten P, Goldberg M. Dentonin, a MEPE fragment, initiates pulp- healing response to injury. J Dent Res 2007; 86: 780-785.
• 19. Koike T, Polan MA, Izumikawa M, Saito T. Induction of reparative dentin formation on exposed dental pulp by dentin phosphophoryn/collagen composite. BioMed Res Int 2014; 2014: 745139.
• 20. Tarim B, Hafez AA, Cox CF. Pulpal response to a resin- modified glass-ionomer material on nonexposed and exposed monkey pulps. Quintessence Int 1998; 29: 535-542.
• 21. do Nascimento AB, Fontana UF, Teixeira HM, Costa CA. Biocompatibility of a resin-modified glass-ionomer cement applied as pulp capping in human teeth. Am J Dent 2000; 13: 28-34.
• 22. Gandolfi, M.G.; Siboni, F.; Botero, T.; Bossù, M.; Riccitiello, F.; Prati, C. Calcium silicate and calcium hydroxide materials for pulp capping: Biointeractivity, porosity, solubility and bioactivity of current formulations. J. Appl. Biomater. Funct. Mater. 2015, 13, 41–60.
• 23. Mickenautsch, S.; Yengopal, V.; Banerjee, A. Pulp response to resin-modified glass ionomer and calcium hydroxide cements in deep cavities: A quantitative systematic review. Dent. Mater. 2010; 26, 761–770.
• 24. Torabinejad M, White DJ, inventors. Tooth filling material and method of use. patent United States Patent & Trademark Office 5,415,547. 1995.
• 25. Noriyasu Hosoya, Tomoyoshi Takigawa, Taku Horie at all. A review of the literature on the efficacy of mineral trioxide aggregate in conservative dentistry. Dental Materials Journal 2019; 38(5): 693–700.
• 26. Aguilar P, Linsuwanont P. Vital pulp therapy in vital permanent teeth with cariously exposed pulp: a systematic review. J Endod 2011; 37: 581-587.
• 27. Pashley DH. Dynamics of the pulpo-dentin complex. Crit Rev Oral Biol Med 1996; 7: 104-133.
• 28. Bergenholtz G. Advances since the paper by Zander and Glass (1949) on the pursuit of healing methods for pulpal exposures: historical perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 100: S102-S108.
• 29. Bogen G, Kim JS, Bakland LK. Direct pulp capping with mineral trioxide aggregate: an observational study. Journal of American Dental Association 2008;139, 305–15.
• 30. Marques MS, Wesselink PR, Shemesh H. Outcome of direct pulp capping with mineral trioxide aggregate: a prospective study. Journal of Endodontics 2015; 41,1026–31.
• 31. Bjørndal L. Caries pathology and management in deep stages of lesion formation. In: Bjørndal L, Kirkevang L-L, Whitworth J, eds. Textbook of Endodontology, 3rd edn. Oxford, 2018; UK. Wiley Blackwell, pp. 61–78.
• 32. L Bjørndal, S. Simon, P. L. Tomson & H. F. Duncan. Management of deep caries and the exposed pulp. International Endodontic Journal 2019; 52, 949–973.
• 33. Bjørndal L, Fransson H, Bruun G et al.. Randomized clinical trials on deep carious lesions: 5-year follow-up. Journal of Dental Research 2017; 96, 747–53.
• 34. Asgary S, Ahmadyar M. Vital pulp therapy using calcium-enriched mixture: An evidence-based review. Journal of Conservative Dentistry 2013;16, 92–8.
• 35. M. Parirokh, M. Torabinejad & P. M. H. Dummer. Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview – part I: vital pulp therapy. International Endodontic Journal 2018; 51, 177–205.
• 36. Asgary S, Nourzadeh M, Eghbal MJ. Miniature pulpotomy of symptomatic mature permanent teeth, a report of two cases. Iranian Endodontic Journal 2016; 11, 75–8.
• 37. Mente J, Hufnagel S, Leo M et al..Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping, long-term results. Journal of Endodontics 2014;40, 1746–51.
• 38. Çalışkan MK, Güneri P. Prognostic factors indirect pulp capping with mineral trioxide aggregate or calcium hydroxide, 2- to 6-year follow-up. Clinical Oral Investigation 2017; 21, 357–67.
• 39. Jang Y, Song M, Yoo IS, Song Y, Roh BD, Kim E. A randomized controlled study of the use of ProRoot mineral trioxide aggregate and Endocem as direct pulp capping materials, 3-month versus 1-year outcomes. Journal of Endodontics 2015; 41, 1201–6.
• 40. Mente J, Geletneky B, Ohle M et al. Mineral trioxide aggregate or calcium hydroxide direct pulp capping, an analysis of the clinical treatment outcome. Journal of Endodontics 2010; 36, 806–13.
• 41. Cho SY, Seo DG, Lee SJ, Lee J, Lee SJ, Jung IY. Prognostic factors for clinical outcomes according to time after direct pulp capping. Journal of Endodontics 2013; 39, 327–31.
• 42. CF, Bergenholtz G, Heys DR, Syed SA, Fitzgerald M, Heys RJ. Pulp capping of dental pulp mechanically exposed to oral microflora: a 1-2 year observation of wound healing in the monkey. Journal of Oral Pathology 1985;14, 156–68.
• 43. Nair PNR, Duncan HF, Pitt Ford TR, Luder HU. Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental cap- ping with mineral trioxide aggregate: a randomized controlled trial. International Endodontic Journal 2008; 41, 128–50.
• 44. Baldissera EZ, Silva AF, Gomes AP et al. Tenascin and fibronectin expression after pulp capping with differ- ent hemostatic agents: a preliminary study. Brazilian Dental Journal 2013; 24, 188–93.
• 45. Tuzuner T, Alacam A, Altunbas DA, Gokdogan FG, Gundogdu E. Clinical and radiographic outcomes of direct pulp capping therapy in primary molar teeth following haemosta- sis with various antiseptics: a randomised controlled trial. European Journal of Paediatric Dentistry 2012; 13, 289–92.
• 46. Galler KM. Clinical procedures for revitalization: current knowledge and considerations. International Endodontic Journal 2016; 49, 926–36.
• 47. Thomas Giraud, Charlotte Jeanneau, Charlotte Rombouts, Hengameh Bakhtiar, Patrick Laurent, Imad About. Pulp capping materials modulate the balance between inflammation and regeneration. Dent Materials 2019; 35(1):24-35.
• 48. Kim I, Moon SO, Kim SH, Kim HJ, Koh YS, Koh GY. Vascular endothelial growth factor expression of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin through nuclear factor-kappa B activation in endothelial cells. J Biol Chem 2001; 276: 7614–20.
• 49. Giraud T, Jeanneau C, Bergmann M, Laurent P, About I. Tricalcium silicate capping materials modulate pulp healing and inflammatory activity in vitro. J Endod 2018; 44(11):1686-1691.
• 50. Laurent, P.; Camps, J.; About, I. BiodentineTM induces TGF-β1 release from human pulp cells and early dental pulp mineralization. Int Endod J 2012; 45: 439–448.
• 51. About I. Dentin–pulp regeneration: the primordial role of the microenvironment and its modification by traumatic injuries and bioactive materials. Endod Top 2013:28, http://dx.doi.org/10.1111/etp.12038.
• 52. Mathieu S, Jeanneau C, Sheibat-Othman N, Kalaji N, Fessi H, About I. Usefulness of controlled release of growth factors in investigating the early events of dentin-pulp regeneration. J Endod 2013; 39: 228–35.
• 53. Zhang W, Walboomers XF, Jansen JA. The formation of tertiary dentin after pulp capping with a calcium phosphate cement, loaded with PLGA microparticles containing TGF-beta1. J Biomed Mater Res A 2008;85:439–44.
• 54. Smith AJ, Scheven BA, Takahashi Y, Ferracane JL, Shelton RM, Cooper PR. Dentine as a bioactive extracellular matrix. Archives of Oral Biology 2012; 57, 109–21.
• 55. Piva E, Silva AF, Nor JE. Functionalized scaffolds to control dental pulp stem cell fate. Journal of Endodontics 2014; 40, 33-40.
• 56. Smith AJ, Duncan HF, Diogenes A, Simon S, Cooper PR. Exploiting the Bioactive Properties of the Dentin-Pulp Complex in Regenerative Endodontics. Journal of Endodontics 2016; 42, 47–56.
• 57. W.L.O da Rosa, E. Piva, A.F. Silva. Disclosing the physiology of pulp tissue for vital pulp therapy. International endodontic journal 2018; 51(8):829-846.
• 58. Farges JC, Alliot-Licht B, Renard E et al. Dental pulp defence and repair mechanisms in dental caries. Mediators of Inflammation, 2015;230251. https://doi.org/10.1155/2015/230251
• 59. Li D, Fu L, Zhang Y et al. The effects of LPS on adhesion and migration of human dental pulp stem cells in vitro. Journal of Dentistry 2014;42, 1327–34.
• 60. Renard E, Gaudin A, Bienvenu G et al. Immune cells and molecular networks in experimentally induced pulpitis. Journal of Dental Research 2016; 95, 196–205.
• 61. Duncan HF, Smith AJ, Fleming GJ, Cooper PR. Histone deacetylase inhibitors epigenetically promote reparative events in primary dental pulp cells. Experimental Cell Research 2013; 319, 1534–43.
• 62. Gervois P, Struys T, Hilkens P et al. Neurogenic maturation of human dental pulp stem cells following neurosphere generation induces morphological and electrophysiological characteristics of functional neurons. Stem Cells and Development 2015; 24, 296–311.
• 63. Simon S, Smith AJ, Lumley PJ et al. Molecular characterization of young and mature odontoblasts. Bone 2009; 45, 693–703.
• 64. Frozoni M, Zaia AA, Line SR, Mina M. Analysis of the contribution of nonresident progenitor cells and hematopoietic cells to reparative dentinogenesis using parabiosis model in mice. Journal of Endodontics 2012; 38, 1214–9.
• 65. Jeanneau C, Lundy FT, El Karim IA, About I. Potential therapeutic strategy of targeting pulp fibroblasts in dentin-pulp regeneration. Journal of Endodontics 2017;43, S17– 24.
• 66. Yoshiba N, Edanami N, Tohma A et al. Detection of bone marrow-derived fibrocytes in human dental pulp repair. International Endodontic Journal 2018; 51, 1187–95. 67. Fitzgerald M, Chiego DJ Jr, Heys DR. Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth. Archives of Oral Biology 1990; 35, 707–15.
• 68. Machado CV, Passos ST, Campos TM et al. The dental pulp stem cell niche based on aldehyde dehydrogenase 1 expression. International Endodontic Journal,2016; 49, 755–63.
• 69. Feng J, Mantesso A, De Bari C, Nishiyama A, Sharpe PT. Dual origin of mesenchymal stem cells contribut- ing to organ growth and repair. Proceedings of the National Academy of Science USA 2011; 108, 6503–8.
• 70. Simon S, Smith AJ. Regenerative endodontics. British Dental Journal,2014; 216, E13.
• 71. Liang Chen and Byoung In Suh. Cytotoxicity and biocompatibility of resin-free and resin-modified direct pulp capping materials: A state-of-the-art review. Dental Materials Journal 2017; 36(1): 1–7.
• 72. Mohammadi Z, Dummer PM. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int Endod J 2011; 44: 697-730.
• 73. Chen L, Shen H, Suh BI. Bioactive dental restorative materials: a review. Am J Dent 2013; 26: 219-227.
• 74. Poggio C, Ceci M, Dagna A, Beltrami R, Colombo M, Chiesa M. In vitro cytotoxicity evaluation of different pulp capping materials: a comparative study. Arh Hig Rada Toksikol 2015; 66: 181-188.
• 75. Prosser HJ, Groffman DM, Wilson AD. The effect of composition on the erosion properties of calcium hydroxide cements. J Dent Res 1982; 61: 1431-1435.
• 76. Furey A, HjelmhaugJ BS, Lobner D. Flow Line, Durafill VS, and Dycal toxicity to dental pulp cells: effects of growth factors. J Endod 2010; 36: 1149-1153.
• 77. Cengiz E, Yilmaz HG. Efficacy of erbium, chromium-doped: yttrium, scandium, gallium, and garnet laser irradiation combined with resin-based tricalcium silicate and calcium hydroxide on direct pulp capping: a randomized clinical trial. J Endod 2016; 42: 351-355.
• 78. Al-Hiyasat AS1, Barrieshi-Nusair KM, Al-Omari MA. The radiographic outcomes of direct pulp-capping procedures performed by dental students: a retrospective study. J Am Dent Assoc 2006; 137: 1699-1705.
• 79. Barthel CR, Rosenkranz B, Leuenberg A, Roulet JF. Pulp capping of carious exposures: treatment outcome after 5 and 10 years: a retrospective study. J Endod 2000; 26: 525-528.
• 80. Jontell M, Hanks C.T., Bratell J, Bergenholtz G. Effects of unpolymerized resin components on the function of accessory cells derived from the rat incisor pulp. J Dent Res 1995; 74: 1162-1167.
• 81. Aranha AM, Giro EM, Hebling J, Lessa FC, Costa CA. Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells. J Appl Oral Sci 2010; 18: 461-466.
• 82. Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Ford TRP. The constitution of mineral trioxide aggregate. Dent Mater Off Publ Acad Dent Mater 2005; 21:297–303.
• 83. Sarkar, N.; Caicedo, R.; Ritwik, P. Physicochemical Basis of the Biologic Properties of Mineral Trioxide Aggregate. J. Endod. 2005; 3, 97–100.
• 84. Parirokh, M.; Torabinejad, M. Mineral Trioxide Aggregate: A Comprehensive Literature Review—Part III: Clinical Applications, Drawbacks, and Mechanism of Action. J. Endod. 2010, 36: 400–413.
• 85. Antunes Bortoluzzi, E.; Sivieri Araújo, G.; Maria Guerreiro Tanomaru, J.; Tanomaru-Filho, M. Marginal Gingiva Discoloration by Gray MTA: A Case Report. J. Endod. 2007; 33: 325–327.
• 86. Felman, D.; Parashos, P. Coronal tooth discoloration and white mineral trioxide aggregate. J. Endod. 2013; 39: 484–487.
• 87. Ha, W.N.; Nicholson, T.; Kahler, B.; Walsh, L.J. Mineral trioxide aggregate-A review of properties and testing methodologies. Materials 2017; 10: 1261.
• 88. Salem-Milani, A.; Ghasemi, S.; Rahimi, S.; Ardalan-Abdollahi, A.; Asghari-Jafarabadi, M. The Discoloration effect of White Mineral Trioxide Aggregate (WMTA), Calcium Enriched Mixture (CEM), and Portland Cement (PC) on Human Teeth. J. Clin. Exp. Dent. 2017; 9: e1397–e1401.
• 89. Schembri, M.; Peplow, G.; Camilleri, J. Analyses of heavy metals in mineral trioxide aggregate and Portland cement. J. Endod. 2010; 36: 1210–1215.
• 90. Hilton, T.J.; Ferracane, J.L.; Mancl, L. Comparison of CaOH with MTA for Direct Pulp Capping: A PBRN Randomized Clinical Trial. Ja. Dent. Res., 2013; 92 :16–22.
• 91. Daniele, L. Mineral Trioxide Aggregate (MTA) direct pulp capping: 10 years clinical results. G. Ital. Endod. 2017; 31: 48–57.
• 92. Yasuda Y, Ogawa M, Arakawa T, Kadowaki T, Saito T. The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study. J Endod 2008; 34: 1057-1060.
• 93. Orhan EO, Maden M, Senguüven B. Odontoblast-like cell numbers and reparative dentine thickness after direct pulp capping with platelet-rich plasma and enamel matrix derivative, a histomorphometric evaluation. International Endodontic Journal 2012; 45, 317–25.
• 94. Obeid M, Saber Sel D, Ismael Ael D, Hassanien E. Mesenchymal stem cells promote hard-tissue repair after direct pulp capping. Journal of Endodontics 2013;39, 626–31.
• 95. Moazzami F, Ghahramani Y, Tamaddon AM, Dehghani Nazhavani A, Adl A. A histological comparison of a new pulp capping material and mineral trioxide aggre-gate in rat molars. Iranian Endodontic Journal 2014; 9, 50–5.
• 96. Parirokh M, Asgary S, Eghbal MJ, Kakoei S, Samiee M. A comparative study of using a combination of calcium chloride and mineral trioxide aggregate as the pulp-capping agent on dogs’ teeth. Journal of Endodontics 2011;37, 786–8.
• 97. Manochehrifar H, Parirokh M, Kakooei S et al. The effect of mixed with chlorhexidine as the pulp capping agent in dogs teeth, a histologic study. Iranian Endodontic Journal 2016; 11, 316–20.
• 98. Ko H, Yang W, Park K, Kim M. Cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and response of rat pulp to MTA and BMP-2. Oral Surgery Oral Medicine Oral Pathology Oral Radiology Endodontics 2010;109, e103–8. 99. Al-Hezaimi K, Al-Tayar BA, Bajuaifer YS, Salameh Z, Al- Fouzan K, Tay FR. A hybrid approach to direct pulp capping by using emdogain with a capping material. Journal of Endodontics 2011; 37, 667–72.
• 100. Bollu IP, Velagula LD, Bolla N, Kumar KK, Hari A, Thumu J. Histological evaluation of mineral trioxide aggregate and enamel matrix derivative combination in direct pulp capping: an in vivo study. Journal of Conservative Dentistry 2016; 19, 536–40.
• 101. Moradi S, Saghravanian N, Moushekhian S, Fatemi S, Forghani M. Immunohistochemical evaluation of fibronectin and tenascin following direct pulp capping with mineral trioxide aggregate, platelet-rich plasma and propolis in dogs’ teeth. Iranian Endodontic Journal 2015;10, 188–92.
• 102. Shahravan A, Jalali SP, Torabi M, Haghdoost AA, Gorjestani H. A histological study of pulp reaction to various water/powder ratios of white mineral trioxide aggregate as pulp-capping material in human teeth, a double-blinded, randomized controlled trial. International Endodontic Journal 2011; 44, 1029–33. 103. Yelamali, S.; Patil, A.C. Evaluation of shear bond strength of a composite resin to white mineral trioxide aggregate with three different bonding systems-An in vitro analysis. J Clin Exp Dent 2016; 8: e273–e277.
• 104. Kaup, M.; Schäfer, E.; Dammaschke, T. An in vitro study of different material properties of Biodentine compared to ProRoot MTA. Head Face Med. 2015; 11, 16.
• 105. Choi, Y.; Park, S.J.; Lee, S.H.; Hwang, Y.C.; Yu, M.K.; Min, K.S. Biological effects and washout resistance of a newly developed fast-setting pozzolan cement. J. Endod. 2013; 39: 467–472.
• 106. Vivan, R.R.; Zapata, R.O.; Zeferino, M.A.; Bramante, C.M.; Bernardineli, N.; Garcia, R.B.; et al. Evaluation of the physical and chemical properties of two commercial and three experimental root-end filling materials. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2010; 110: 250–256.
• 107. Pornamazeh, T.; Yadegari, Z.; Ghasemi, A.; Sheykh-al-Eslamian, S.M.; Shojaeian, S.H. In Vitro cytotoxicity and setting time assessment of calcium-enriched mixture cement, retro mineral trioxide aggregate and mineral trioxide aggregate. Iran. Endod. J. 2017; 12, 488–492.
• 108. Camilleri J, Sorrentino F, Damidot D. Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater 2013; 29: 580-593.
• 109. Dammaschke, T.; Nowicka, A.; Lipski, M.; Ricucci, D. Histological evaluation of hard tissue formation after direct pulp capping with a fast-setting mineral trioxide aggregate (RetroMTA) in humans. Clin. Oral Investig. 2019; 23: 4289–4299.
• 110. Guo, Y.J.; Du, T.F.; Li, H.B.; Shen, Y.; Mobuchon, C.; Hieawy, A.; Wang, Z.J.; Yang, Y.; Ma, J.; Haapasalo, M. Physical properties and hydration behavior of a fast-setting bioceramic endodontic material. BMC Oral Health 2016; 16, 1–6.
• 111. Nekoofar, M.H.; Aseeley, Z.; Dummer, P.M.H. The effect of various mixing techniques on the surface microhardness of mineral trioxide aggregate. Int Endod J 2010; 43, 312–320.
• 112. Chedella, S.C.V.; Berzins, D.W. A differential scanning calorimetry study of the setting reaction of MTA. Int Endod J 2010; 43, 509–518.
• 113. Dawood AE, Parashos P, Wong RHK, Reynolds EC, Manton DJ. Calcium silicate-based cements: composition, properties, and clinical applications. J Investig Clin Dent 2017; 8: 12195.
• 114. Vallés M, Roig M, Duran-Sindreu F, Martínez S, Mercadé M. Color stability of teeth restored with biodentine: a 6-month in vitro study. J Endod 2015; 41: 1157–60.
• 115. Kum KY, Kim EC, Yoo YJ, Zhu Q, Safavi K, Bae KS, Chang SW. Trace metal contents of three tricalcium silicate materials: MTA Angelus, Micro Mega MTA and Bioaggregate. Int Endod J 2014; 47: 704-710.
• 116. Yalcin M, Arslan U, Dundar A. Evaluation of antibacterial effects of pulp capping agents with direct contact test method. Eur J Dent 2014; 8: 95-99.
• 117. Saeed A., Mohammad Jafar Eghbal, Masoud Parirokh, and Hassan Torabzadeh. Sealing Ability of Three Commercial Mineral Trioxide Aggregates and an Experimental Root-End Filling Material. Iran Endod J. 2006; 1(3): 101–105.
• 118. Burcu Oğlakçı, Neslihan Arhun, Duygu Tuncer. Pulp cappping treatments in restorative dentistry. J Dent Fac Atatürk Uni. 2016; 14:94-103.
• 119. Gudkina J, Mindere A, Locane G, Brinkmane A. Review of the success of pulp exposure treatment of cariously and traumatically exposed pulps in immature permanent incisors and molars. Stomatologija 2012; 14:71-80.
• 120. Mihriban Gökçek, Ebru Hazar Bodrumlu. New approaches in vital pulp therapy. J Dent Fac Atatürk Uni 2016; 14:118-129.
• 121. Asgary S, Eghbal MJ, Parirokh M, Ghoddusi J, Kheirieh S, Brink F. Comparison of Mineral Trioxide Aggregate's Composition with Portland Cements and a New Endodontic Cement. J Endod 2009; 35:243–50.
• 122. Zarrabi MH, Javidi M, Jafarian AH, Joushan B. Histologic assessment of human pulp response to capping with mineral trioxide aggregate and a novel endodontic cement. Journal of Endodontics 2010; 36:1778–81.
• 123. Siboni, F.; Taddei, P.; Prati, C.; Gandolfi, M.G. Properties of NeoMTA plus and MTA plus cements for endodontics. Int. Endod. J. 2017; 50: e83–e94.
• 124. Camilleri, J. Staining Potential of Neo MTA Plus, MTA Plus, and Biodentine Used for Pulpotomy Procedures. J Endod 2015; 41: 1139–1145.
• 125. Zeid, S.T.A.; Alamoudi, N.M.; Khafagi, M.G.; Abou Neel, E.A. Chemistry and Bioactivity of NeoMTA Plus versus MTA Angelus Root Repair Materials. J Spectrosc 2017; 2017, 8736428. 126. Mahgoub, N.; Alqadasi, B.; Aldhorae, K.; Assiry, A.; Altawili, Z.; Hong, T. Comparison between iRoot BP Plus (EndoSequence Root Repair Material) and Mineral Trioxide Aggregate as Pulp-capping Agents: A Systematic Review. J Int Soc Prev Community Dent 2019; 9: 542–552.
• 127. Rao, Q.; Kuang, J.; Mao, C.; Dai, J.; Hu, L.; Lei, Z.; Song, G.; Yuan, G. Comparison of iRoot BP Plus and Calcium Hydroxide as Pulpotomy Materials in Permanent Incisors with Complicated Crown Fractures: A Retrospective Study. J Endod 2020; 46(3):352-357.
• 128. Loushine BA, Bryan TE, Looney SW, Gillen BM, Loushine RJ, Weller RN, Pashley DH, Tay FR. Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer. J Endod 2011; 37: 673-677. 129. Damas BA, Wheater MA, Bringas JS, Hoen MM. Cytotoxicity comparison of mineral trioxide aggregates and EndoSequence bioceramic root repair materials. J Endod 2011; 37: 372-375.
• 130. Ma J, Shen Y, Stojicic S, Haapasalo M. Biocompatibility of two novel root repair materials. J Endod 2011; 37: 793-798.
• 131. Shi S, Bao ZF, Liu Y, Zhang DD, Chen X, Jiang LM, Zhong M. Comparison of in vivo dental pulp responses to capping with iRoot BP Plus and mineral trioxide aggregate. Int Endod J 2016; 49: 154-160.
• 132. Liu S, Wang S, Dong Y. Evaluation of a bioceramic as a pulp capping agent in vitro and in vivo. J Endod 2015; 41: 652- 657.
• 133. Rajasekharan, S.; Martens, L.C.; Cauwels, R.G.E.C.; Verbeeck, R.M.H. Biodentine material characteristics and clinical applications: A review of the literature. Eur Arch Paediatr Dent 2014; 15: 147–158.
• 134. Grech, L.; Mallia, B.; Camilleri, J. Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials. Dent Mater, 2013; 29: e20–e28.
• 135. Fonseca, T.S.; Silva, G.F.; Jm, G.; In, C.P.S. In Vivo evaluation of the inflammatory response and IL-6 immunoexpression promoted by Biodentine and MTA Angelus. Int Endod J 2015; 49: 1–9.
• 136. Aeinehchi M, Eslami B, Ghanbariha M, Saffar AS. Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: a preliminary report. Int Endod J 2003; 36: 225-231.
• 137. Okiji T, Yoshiba K. Reparative dentinogenesis induced by mineral trioxide aggregate: a review from the biological and physicochemical points of view. Int J Dent 2009; 464280.
• 138. About, I. Biodentine: From biochemical and bioactive properties to clinical applications.G. Ital. Endod. 2016; 30: 81–88.
• 139. Natale LC, Rodrigues MC, Xavier TA, Simões A, de Souza DN, Braga RR. Ion release and mechanical properties of calcium silicate and calcium hydroxide materials used for pulp capping. Int Endod J 2015; 48: 89-94.
• 140. Tanalp, J.; Karapınar-Kazandag, M.; Dölekoglu, S.; Kayahan, M.B. Comparison of the radiopacities of different root-end filling and repair materials. Sci World J 2013, 2013, 594950.
• 141. Aksoy, M.K.; Oz, F.T.; Orhan, K. Evaluation of calcium (Ca2+) and hydroxide (OH−) ion diffusion rates of indirect pulp capping materials. Int J Artif. Organs 2017; 40: 641–646.
• 142. Kaup, M.; Dammann, C.H.; Schäfer, E.; Dammaschke, T. Shear bond strength of Biodentine, ProRoot MTA, glass ionomer cement and composite resin on human dentine ex vivo. Head Face Med. 2015; 11: 14.
• 143. Nowicka A, Lipski M, Parafiniuk M, Sporniak-Tutak K, Lichota D, Kosierkiewicz A, et al. Response of human dental pulp capped with biodentine and mineral trioxide aggregate. J Endod 2013; 39: 743-747.
• 144. Kaur, M.; Singh, H.; Dhillon, J.S.; Batra, M.; Saini, M.MTA versus Biodentine: Review of Literature with a Comparative Analysis. J Clin Diagn Res 2017; 11: ZG01–ZG05.
• 145. Hegde, S.; Sowmya, B.; Mathew, S.; Bhandi, S.H.; Nagaraja, S.; Dinesh, K. Clinical evaluation of mineral trioxide aggregate and biodentine as direct pulp capping agents in carious teeth. J Conserv Dent 2017; 20: 91–95.
• 146. Awawdeh, L.; Al-Qudah, A.; Hamouri, H.; Chakra, R.J. Outcomes of Vital Pulp Therapy Using Mineral Trioxide Aggregate or Biodentine: A Prospective Randomized Clinical Trial. J Endod 2018; 44: 1603–1609.
• 147. Brizuela, C.; Ormeño, A.; Cabrera, C.; Cabezas, R.; Silva, C.I.; Ramírez, V.; Mercade, M. Direct Pulp Capping with Calcium Hydroxide, Mineral Trioxide Aggregate, and Biodentine in Permanent Young Teeth with Caries: A Randomized Clinical Trial. J Endod 2017; 43: 1776–1780.
• 148. Katge, F.A.; Patil, D.P. ComparativeAnalysis of 2Calcium Silicate—Based Cements (Biodentine and Mineral Trioxide Aggregate) as Direct Pulp-capping Agent in Young Permanent Molars: A Split Mouth Study. J Endod 2017; 43: 507–513.
• 149. Lipski, M.; Nowicka, A.; Kot, K.; Postek-Stefan ́ska, L.; Wysoczan ́ska-Jankowicz, I.; Borkowski, L.; et al. Factors affecting the outcomes of direct pulp capping using Biodentine. Clin Oral Investig 2018; 20: 2021–2029.
• 150. Mahmoud, S.; El-Negoly, S.; ZaenEl-Din, A.; El-Zekrid, M.; Grawish, L.; Grawish, H.; Grawish, M. Biodentine versus mineral trioxide aggregate as a direct pulp capping material for human mature permanent teeth—A systematic review. J Conserv Dent 2018; 21: 466.
• 151. Bakhtiar, H.; Nekoofar, M.H.; Aminishakib, P.; Abedi, F.; Naghi Moosavi, F.; Esnaashari, et al. Human Pulp Responses to Partial Pulpotomy Treatment with TheraCal as Compared with Biodentine and ProRoot MTA: A Clinical Trial. J Endod 2017; 43: 1786–1791.
• 152. Paula, A.; Laranjo, M.; Marto, C.M.; Abrantes, A.M.; Casalta-Lopes, J.; Gonçalves, A.C.; et al. Biodentine Boosts, WhiteProRoot MTA Increases and Life Suppresses Odontoblast Activity. Materials 2019; 12: 1184.
• 153. Kim, J.; Song, Y.-S.; Min, K.-S.; Kim, S.-H.; Koh, J.-T.; Lee, B.-N.; et al. Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry. Restor Dent Endod 2016; 41: 29.
• 154. Tulumbaci, F.; Almaz, M.E.; Arikan, V.; Mutluay, M.S. Shear bond strength of different restorative materials to mineral trioxide aggregate and Biodentine. J Conserv Dent 2017; 20: 292–296.
• 155. Ha, H.-T. The effect of the maturation time of calcium silicate-based cement (Biodentine) on resin bonding: An in vitro study. Appl Adhes Sci 2019 7, 1.
• 156. Cantekin, K.; Avci, S. Evaluation of shear bond strength of two resin-based composites and glass ionomer cement to pure tricalcium silicate-based cement (Biodentine). J Appl Oral Sci 2014; 22: 302–306.
• 157. Hashem, D.F.; Foxton, R.; Manoharan, A.; Watson, T.F.; Banerjee, A. The physical characteristics of resin composite–calcium silicate interface as part of a layered/laminate adhesive restoration. Dent Mater 2014; 30: 343–349.
• 158. Sultana, N.; Nawal, R.; Chaudhry, S.; Sivakumar, M.; Talwar, S. Effect of acid etching on the micro-shear bond strength of resin composite–calcium silicate interface evaluated over different time intervals of bond aging. J Conserv Dent 2018; 21: 194–197.
• 159. Asgary S, Eghbal MJ, Parirokh M, Ghanavati F, Rahimi H. A comparative study of histologic response to different pulp capping materials and a novel endodontic cement. Oral Surgery Oral Medicine Oral Pathology Oral Radiology Endodontics 2008;106, 609–14.
• 160. Tziafa C, Koliniotou-Koumpia E, Papadimitriou S, Tziafas D. Dentinogenic responses after direct pulp capping of miniature swine teeth with Biodentine. Journal of Endodontics,2014; 40, 1967–71.
• 161. Gandolfi MG, Siboni F, Prati C. Chemical-physical properties of TheraCal, a novel light-curable MTA-like material for pulp capping. Int Endod J 2012; 45: 571-579.
• 162. Camilleri, J.; Laurent, P.; About, I. Hydration of Biodentine, Theracal LC, and a Prototype Tricalcium Silicate–based Dentin Replacement Material after Pulp Capping in Entire Tooth Cultures. J Endod, 2014; 40: 1846–1854.
• 163. Fathy, S. Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model. J Clin Exp Dent 2019; 11: e360–e366.
• 164. Camilleri, J. Hydration characteristics of Biodentine and Theracal used as pulp capping materials. Dent Mater 2014; 30: 709–715.
• 165. Diamanti E, Mathieu S, Jeanneau C, Kitraki E, Panopoulos P, Spyrou G, et al. Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA. Int Endod J 2013;46: 160–8.
• 166. Kamal, E.; Nabih, S.; Obeid, R.; Abdelhameed, M. The reparative capacity of different bioactive dental materials for direct pulp capping. Dent Med Probla 2018; 55: 147–152.
• 167. Savas, S.; Botsali, M.S.; Kucukyilmaz, E.; Sari, T. Evaluation of temperature changes in the pulp chamber during polymerization of light-cured pulp-capping materials by using a VALO LED light curing unit at different curing distances. Dent Mater J 2014;33: 764–769.
• 168. Gomes-Filho JE, de Faria MD, Bernabé PF, Nery MJ, Otoboni- Filho JA, Dezan-Júnior E, et al. Mineral trioxide aggregate but not light-cure mineral trioxide aggregate stimulated mineralization. J Endod 2008; 34: 62- 65.
• 169. Jeanneau, C.; Laurent, P.; Rombouts, C.; Giraud, T.; About, I. Light-cured Tricalcium Silicate Toxicity tot he Dental Pulp. J Endod 2017; 43: 2074–2080.
• 170. Li, X.; DeMunck, J.; VanLanduyt, K.; Pedano, M.; Chen, Z.; VanMeerbeek, B. How effectively do hydraulic calcium-silicate cements remineralize demineralized dentin. Dent Mater 2017, 33, 434–445.
• 171. Nielsen MJ, Casey JA, VanderWeele RA, Vandewalle KS. Mechanical properties of new dental pulp-capping materials. Gen Dent 2016; 64: 44-48.
• 172. Poggio C, Arciola CR, Beltrami R, Monaco A, Dagna A, Lombardini M, Visai L. Cytocompatibility and antibacterial properties of capping materials. Sci World J 2014; 181945: 1-10.
• 173. Bortoluzzi EA, Niu LN, Palani CD, El-Awady AR, Hammond BD, Pei DD, et al. Cytotoxicity and osteogenic potential of silicate calcium cements as potential protective materials for pulpal revascularization. Dent Mater 2015; 31: 1510-1522.
• 174. Lee, H.; Shin, Y.; Kim, S.-O.; Lee, H.-S.; Choi, H.-J.; Song, J.S. Comparative Study of Pulpal Responses to Pulpotomy with ProRoot MTA, RetroMTA, and TheraCal in Dogs’ Teeth. J Endod 2015; 41: 1317–1324.
• 175. Duarte MA, De Oliveira Demarchi AC, Yamashita JC, Kuga MC, De Campos Fraga S. Arsenic release provided by MTA and Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 99: 648-650.
• 176. Chang SW, Baek SH, Yang HC, Seo DG, Hong ST, Han SH, et al. Heavy metal analysis of ortho MTA and ProRoot MTA. J Endod 2011; 37: 1673-1676.
• 177. Demirkaya K, Can Demirdöğen B, Öncel Torun Z, Erdem O, Çetinkaya S, Akay C. In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels in rats. Eur J Oral Sci 2016; 124: 75-81.
• 178. Makkar, S.; Kaur, H.; Aggarwal, A.; Vashish, R. A confocal laser scanning microscopic study evaluating the sealing ability of mineral trioxide aggregate, Biodentine and new pulp capping agent—TheraCal. Dent J Adv Stud 2015; 3: 20–25.
• 179. Meraji, N.; Camilleri, J. Bonding over Dentin Replacement Materials. J Endod 2017;43: 1343–1349.
• 180. Karadas, M.; Cantekin, K.; Gumus, H.; Ates ̧, S.M.; Duymus ̧, Z.Y. Evaluation of the bond strength of different adhesive agents to a resin-modified calcium silicate material (TheraCal LC). Scanning 2016; 38: 403–411. 181. Jun, S.-K.; Lee, J.-H.; Lee, H.-H. The Biomineralization of a Bioactive Glass-Incorporated Light-Curable Pulp Capping Material Using Human Dental Pulp Stem Cells. Biomed Res Int 2017, 2017, 2495282.
• 182. Abou ElReash, A.; Hamama, H.; Abdo, W.; Wu, Q.; Zaen El-Din, A.; Xiaoli, X. Biocompatibility of new bioactive resin composite versus calcium silicate cements: An animal study. BMC Oral Health 2019, 19(1): 194.
• 183. Faraco Junior IM, Holland R. Histomorphological response of dogs’ dental pulp capped with white mineral trioxide aggregate. Braz Dent J 2004; 15: 104-108.
• 184. Inoue T, Miyakoshi S, Shimono M. The in vitro and in vivo influence of 4-META/MMA-TBB resin components on dental pulp tissues. Adv Dent Res 2001; 15: 101-104.
• 185. Imaizumi N, Kondo H, Ohya K, Kasugai S, Araki K, Kurosaki N. Effects of exposure to 4-META/MMA-TBB resin on pulp cell viability. J Med Dent Sci 2006; 53: 127-133.
• 186. Garza EG, Wadajkar A, Ahn C, Zhu Q, Opperman LA, Bellinger LL, Nguyen KT, Komabayashi T. Cytotoxicity evaluation of methacrylate-based resins for clinical endodontics in vitro. J Oral Sci 2012; 54: 213-217.
• 187. Taira Y, Imai Y. Review of methyl methacrylate (MMA)/ tributylborane (TBB)-initiated resin adhesive to dentin. Dent Mater J 2014; 33: 291-304.
• 188. Ahangari Z, Naseri M, Jalili M, Mansouri Y, Mashhadiabbas F, Torkaman A. Effect of propolis on dentin regeneration and the potential role of dental pulp stem cell inguinea pigs. Cell J 2012; 13:223-8.
• 189. Iraj Yazdanfar, Mehrdad Barekatain, Maryam Zare Jahromi. Combination effects of diode laser and resin-modified tricalcium silicate on direct pulp capping treatment of caries exposures in permanent teeth: a randomized clinical trial. Lasers Med Sci. 2020; 35(8):1849-1855.
• 190. Karaaslan Eş, Yıldırım C, Üşümez A. Restoratif tedavide lazer uygulamaları. Atatürk Üniv Diş Hek Fak Derg 2012; 22:340-9.
• 191. Mengchen W, Lin M, Qian L, Wendong Y. Efficacy of Er:YAG laser-assisted direct pulp capping in permanent teeth with cariously exposed pulp: A pilotstudy.AustEndod J 2020; 46(3):351-357.
• 192. Al-Hezaimi K, Al-Tayar BA, BaJuaifer YS, Salameh Z, Al-Fouzan K, Tay FR. A hybrid approach to direct pulp capping by using emdogain with a capping material. J Endod 2011; 37:667-72.
• 193. Min KS, Yang SH, Kim EC. The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in humandental pulp cells. J Endod 2009; 35:847–51.
• 194. Al-Sanabani JS, Madfa AA, Al-Sanabani FA. Application of calcium phosphate materials in dentistry. Int J Biomater 2013; doi: 10.1155/2013/876132
• 195. Qureshi A, Soujanya E, Nandakumar, Pratapkumar, Sambashivarao. Recent advances in pulp capping materials: an overview. J Clin Diagn Res 2014; 8: 316-21.
• 196. Limjeerajarus CN, Osathanon T, Manokawinchoke J, Pavasant P. Iloprost Up-regulates Vascular Endothelial Growth Factor Expression in Human Dental Pulp Cells In Vitro and Enhances Pulpal Blood Flow In Vivo. J Endod 2014; 40:925-30.
• 197. Nakashima M, Iohara K. Regeneration of dental pulp by stem cells. Adv Dent Res 2011; 23:313–9.
• 198. About I. Recent Trends in Tricalcium Silicates for Vital Pulp Therapy. Curr Oral Health Rep 2018; 5:178–85.
• 199. Rodrigues EM, Cornélio AL, Mestieri LB, Fuentes AS, Salles LP, Rossa-Junior C et al. Human dental pulp cells response to mineral trioxide aggregate (MTA) and MTA Plus: cytotoxicity and gene expression analysis. Int Endod J. 2017 Aug;50(8):780-9.