OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY

Yıl 2020, Cilt: 7 Sayı: 1, 44 - 53, 01.04.2020

Aysan Lektemür Alpan , Hülya Toker , Hatice Özer

https://doi.org/10.15311/selcukdentj.419582

Öz

Amaç: Diabetes
mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone
have several actions such as
antimicrobial ,immunostimulating, antihypoxic effects, and activates
antioxidant enzymes and angiogenesis. The aim of this study is to investigate the effect of gaseous ozone on
bone healing in diabetic rat calvarial defects treated with autogenous bone
graft, morphometrically and immunohistochemically

Gereç ve Yöntemler:Diabetes
was induced and critical size defects were created on rats. Study groups:
1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Empty
defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application
(AG+Ozone, n=14) group. Gaseous ozone was applied on the operation day and the
following 2 weeks daily (140ppm @ 2L/d, 2.24 mg).
Total bone area was measured.
Osteocalcin and
Bone morphogenic
protein-2  protein expressions were evaluated.

Bulgular:Control and Control+Ozone groups had no
osteoclast and residuel lacunae during the study . Osteoblasts in AG+Ozone
group were higher than AG group at 4th week (p>0.05). AG+Ozone group had
more total bone area than AG group at 4th week. AG+Ozone group revealed more
BMP-2 immune positivity compared to the other groups. Osteocalcin immune
positivity in AG groups was higher than those of the Control groups.

Sonuçlar:

Anahtar Kelimeler:

Anahtar Kelimeler

autograft, bone regeneration, calvarial defect, experimental diabetes mellitus

Kaynakça

• REFERENCES 1. Gurav AN. Management of diabolical diabetes mellitus and periodontitis nexus: Are we doing enough? World J Diabetes 2016;7:50-66.
• 2. Jiao H, Xiao E, Graves DT. Diabetes and Its Effect on Bone and Fracture Healing. Curr Osteoporos Rep 2015;13:327-335.
• 3. Daugela P, Cicciu M, Saulacic N. Surgical Regenerative Treatments for Peri-Implantitis: Meta-analysis of Recent Findings in a Systematic Literature Review. J Oral Maxillofac Res 2016;7:e15.
• 4. Dimitriou R, Jones E, McGonagle D, Giannoudis PV. Bone regeneration: current concepts and future directions. BMC Med 2011;9:66.
• 5. Yin W, Qi X, Zhang Y, et al. Advantages of pure platelet-rich plasma compared with leukocyte- and platelet-rich plasma in promoting repair of bone defects. J Transl Med 2016;14:73.
• 6. Peng W, Kim IK, Cho HY, et al. Assessment of the autogenous bone graft for sinus elevation. J Korean Assoc Oral Maxillofac Surg 2013;39:274-282.
• 7. Keskiner I, Alkan A, Acikgoz G, Arpak N, Kaplan S, Arslan H. Platelet-rich plasma and autogenous bone graft combined with guided tissue regeneration in periodontal fenestration defects in dogs. Int J Periodontics Restorative Dent 2014;34:e112-120.
• 8. Kokdere NN, Baykul T, Findik Y. The use of platelet-rich fibrin (PRF) and PRF-mixed particulated autogenous bone graft in the treatment of bone defects: An experimental and histomorphometrical study. Dent Res J (Isfahan) 2015;12:418-424.
• 9. Toker H, Ozdemir H, Ozer H, Eren K. Alendronate enhances osseous healing in a rat calvarial defect model. Arch Oral Biol 2012;57:1545-1550.
• 10. Ozdemir H, Toker H, Balci H, Ozer H. Effect of ozone therapy on autogenous bone graft healing in calvarial defects: a histologic and histometric study in rats. J Periodontal Res 2013;48:722-726.
• 11. Gupta G, Mansi B. Ozone therapy in periodontics. J Med Life 2012;5:59-67.
• 12. Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006;37:425-435.
• 13. Huth KC, Jakob FM, Saugel B, et al. Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 2006;114:435-440.
• 14. Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita M, Nishihara T. Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiol Immunol 2004;19:240-246.
• 15. Huth KC, Quirling M, Lenzke S, et al. Effectiveness of ozone against periodontal pathogenic microorganisms. Eur J Oral Sci 2011;119:204-210.
• 16. Kazancioglu HO, Ezirganli S, Aydin MS. Effects of laser and ozone therapies on bone healing in the calvarial defects. J Craniofac Surg 2013;24:2141-2146.
• 17. Kazancioglu HO, Kurklu E, Ezirganli S. Effects of ozone therapy on pain, swelling, and trismus following third molar surgery. Int J Oral Maxillofac Surg 2014;43:644-648.
• 18. Erdemci F, Gunaydin Y, Sencimen M, et al. Histomorphometric evaluation of the effect of systemic and topical ozone on alveolar bone healing following tooth extraction in rats. Int J Oral Maxillofac Surg 2014;43:777-783.
• 19. Lektemur Alpan A, Toker H, Ozer H. Ozone Therapy Enhances Osseous Healing In Diabetic Rats With Calvarial Defect: A Morphometric and Immunohistochemical Study. J Periodontol 2016:1-15.
• 20. Deliberador TM, Giovanini AF, Lopes TR, et al. Immunoexpression of PPAR-gamma and osteocalcin proteins for bone repair of critical-size defects treated with fragmented autogenous abdominal adipose tissue graft. Braz Dent J 2014;25:179-185.
• 21. Kumar V, Mahdi F, Khanna AK, et al. Antidyslipidemic and Antioxidant Activities of Hibiscus rosa sinensis Root Extract in Alloxan Induced Diabetic Rats. Indian J Clin Biochem 2013;28:46-50.
• 22. Mohammadi Sartang M, Mazloomi SM, Tanideh N, Rezaian Zadeh A. The Effects of Probiotic Soymilk Fortified with Omega-3 on Blood Glucose, Lipid Profile, Haematological and Oxidative Stress, and Inflammatory Parameters in Streptozotocin Nicotinamide-Induced Diabetic Rats. J Diabetes Res 2015;2015:696372.
• 23. Islam MS, Loots du T. Experimental rodent models of type 2 diabetes: a review. Methods Find Exp Clin Pharmacol 2009;31:249-261.
• 24. Srinivas NR. Strategies for preclinical pharmacokinetic investigation in streptozotocin-induced diabetes mellitus (DMIS) and alloxan-induced diabetes mellitus (DMIA) rat models: case studies and perspectives. Eur J Drug Metab Pharmacokinet 2015;40:1-12.
• 25. Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res 1986:299-308.
• 26. Freeman E, Turnbull RS. The value of osseous coagulum as a graft material. J Periodontal Res 1973;8:229-236.
• 27. Petridis X, Diamanti E, Trigas G, Kalyvas D, Kitraki E. Bone regeneration in critical-size calvarial defects using human dental pulp cells in an extracellular matrix-based scaffold. J Craniomaxillofac Surg 2015;43:483-490.
• 28. Vo TN, Ekenseair AK, Spicer PP, et al. In vitro and in vivo evaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering. J Control Release 2015;205:25-34.
• 29. Abreu TC, Lima RP, Souza VS, et al. The biopolymer sugarcane as filling material of critical defects in rats. Acta Cir Bras 2016;31:53-58.
• 30. Gorla LF, Spin-Neto R, Boos FB, Pereira Rdos S, Garcia-Junior IR, Hochuli-Vieira E. Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: a prospective, randomized, volumetric computed tomography study. Int J Oral Maxillofac Surg 2015;44:1486-1491.
• 31. Shyng YC, Devlin H, Sloan P. The effect of streptozotocin-induced experimental diabetes mellitus on calvarial defect healing and bone turnover in the rat. Int J Oral Maxillofac Surg 2001;30:70-74.
• 32. Pacios S, Kang J, Galicia J, et al. Diabetes aggravates periodontitis by limiting repair through enhanced inflammation. FASEB J 2012;26:1423-1430.
• 33. Lu H, Kraut D, Gerstenfeld LC, Graves DT. Diabetes interferes with the bone formation by affecting the expression of transcription factors that regulate osteoblast differentiation. Endocrinology 2003;144:346-352.
• 34. Kan B, Sencimen M, Bayar GR, et al. Histomorphometric and microtomographic evaluation of the effects of hyperbaric oxygen and systemic ozone, used alone and in combination, on calvarial defect healing in rats. J Oral Maxillofac Surg 2015;73:1231 e1231-1210.
• 35. Salazar VS, Gamer LW, Rosen V. BMP signalling in skeletal development, disease and repair. Nat Rev Endocrinol 2016;12:203-221.
• 36. Pacios S, Andriankaja O, Kang J, et al. Bacterial infection increases periodontal bone loss in diabetic rats through enhanced apoptosis. Am J Pathol 2013;183:1928-1935.
• 37. Sarkar PD, Choudhury AB. Relationships between serum osteocalcin levels versus blood glucose, insulin resistance and markers of systemic inflammation in central Indian type 2 diabetic patients. Eur Rev Med Pharmacol Sci 2013;17:1631-1635.
• 38. Zanatta LC, Boguszewski CL, Borba VZ, Kulak CA. Osteocalcin, energy and glucose metabolism. Arq Bras Endocrinol Metabol 2014;58:444-451.
• 39. Starup-Linde J, Vestergaard P. Biochemical bone turnover markers in diabetes mellitus - A systematic review. Bone 2015.
• 40. Roszer T. Inflammation as death or life signal in diabetic fracture healing. Inflamm Res 2011;60:3-10.