Hidroksizin Hidroklorürün Memeli Makrofajları Üzerindeki Etkilerinin İncelenmesi

Abstract

Amaç: Hidroksizin hidroklorür son yıllarda anksiyete bozukluğu, ruhsal sıkıntı ve gerginliğin tedavisinde yaygın olarak tercih edilen antihistaminik türevli bir ilaçtır. Literatürde Hidroksizin hidroklorürün bağışıklık sistemi üzerine etkileri ile ilgili yeterli çalışma mevcut değildir. Gereç ve Yöntemler: Çalışmamızda J774.2 makrofaj hücre hattında lipopolisakkarit (LPS) tehlike sinyali varlığında ve yokluğunda 1, 5,10 ve 20 µg/ml hidroksizin hidroklorür uygulayarak proinflamatuvar sitokin seviyelerine etkilerini araştırdık. İstatistiksel analiz için Student t testi uygulandı (p<0.0001, N=3.) Tüm deney koşulları üçlü olarak test edildi. Bulgular: Hidroksizin hidroklorür makrofaj hücrelerinde 24 saatlik inkübasyondan sonra tümör nekroz alfa (TNFα), interlökin 6 (IL-6), interlökin-12 subunit beta (IL12p40) ve granülosit makrofaj koloni uyarıcı faktör (GM-CSF) sitokin seviyelerini etkilemeyerek bağışıklık hücrelerini aktive etmedi. İmminostimülatör etki göstermeyen bu değerlerde istatistiksel olarak bir fark gözlenmedi. Sonuç: Hidroksizin hidroklorür makrofaj hücreleri için imminostimülatör etki göstermediği için klinikte güvenli bir şekilde kullanılabilinir.

Keywords

• J774.2
• Makrofaj
• Sitokin
• Hidroksizin Hidroklorür

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