Dişi Sıçanlarda Üç Farklı Stres Modelinin Anksiyete ve/veya Depresyon Benzeri Davranışlara Etkilerinin Karşılaştırılması

Abstract

Amaç: Mevcut çalışmada yetişkin dişi sıçanlarda üç farklı stres protokolünün anksiyete/depresyon benzeri davranışlara etkisinin kıyaslanması amaçlanmıştır.

Gereç ve Yöntemler: Yetişkin Wistar sıçanlar kontrol, immobilizasyon stresi-1 (günlük 45 dakika) immobilizasyon stresi-2 (günlük iki kez 45 dakika) ve sosyal izolasyon (metabolik kafeste) olarak rastgele dört gruba ayrıldı (n = 8). Stres protokolleri 10 gün boyunca uygulandı ve depresyon/anksiyete davranışları, hayvanlar diöstrüs dönemindeyken, açık alan ve zorunlu yüzme testi ile değerlendirildi. Aynı testleri 10 günlük dinlenme süresinden sonra tekrarlandı.

Bulgular: Açık alan testinde, merkez alanda geçirilen zaman immobilizasyon stresi-2 ve sosyal izolasyon gruplarında azaldı (p<0.05). Toplam kat edilen mesafe immobilizasyon stresi-1 (p<0.01) ve sosyal izolasyon (p<0.05) gruplarında daha düşüktü. Sosyal izolasyon grubunun şahlanma davranışı (p < 0.05) kontrol grubuna göre düşüktü. İmmobilizasyon stresi-1 ve sosyal izolasyon gruplarında yüzme davranışı azalırken (p < 0.01), immobilizasyon davranışı kontrol grubuna göre artış göstermişti (p <0.05). İkinci testlerde, merkezi alanda geçirilen süre immobilizasyon stresi-1 ve immobilizasyon stresi-2 gruplarında daha düşüktü (p <0.05 ve p <0.01). İmmobilizasyon stresi-1 (p <0.001) ve sosyal izolasyon gruplarında (p <0.01) yüzme davranışı azalırken, immobilizasyon süresi kontrole kıyasla artmıştı (p <0.001).

Sonuç: İmmobilizasyon stresi-1 ve metabolik kafeste sosyal izolasyon protokolü uygulanan erişkin dişi sıçanlarda depresyon benzeri davranışların daha baskın olduğu söylenebilir. 

Keywords

• immobilizasyon stresi,sosyal izolasyon,depresyon,anksiyete,dişi sıçan

Comparison of Effects of Three Distinct Stress Models on Anxiety and/or Depression-Related Behaviors in Female Rats

Abstract

Objective: The aim of the present study was to compare the effects of three distinct stress protocols on anxiety/depression-related behaviors in adult female rats.

Material and Methods: Adult Wistar rats were randomly divided into four groups (n=8) as control, immobilization stress-1 (daily 45 minutes) immobilization stress-2 (daily twice 45 minutes) and social isolation (rats were housed in a metabolic cage). Stress protocols were performed for a period of 10 days. When the animals were in diestrus, depression/anxiety-like behaviors were evaluated by the open field test and forced swimming test. The same tests were repeated after a 10-day rest period.

Results: In the open field test, a percentage of time spent in the central area was lower in the immobilization stress-2 and social isolation (p<0.05) groups and total distance was lower in the immobilization stress-1 (p<0.01) and the social isolation groups (p<0.05). Rearing score was lower in the social isolation group (p<0.05). Swimming behaviors were lower (p<0.01), and immobility durations were higher (p<0.05) in the immobilization stress-1 and social isolation groups. In the second tests, time spent in the central area was lower in the immobilization stress-1 (p<0.05) and the immobilization stress-2 (p<0.01) groups. Swimming behaviors were lower and immobility behaviors were higher in the immobilization stress-1 group (p<0.001) and the social isolation group (p<0.01 and p<0.001, respectively).

Conclusion: We suggest that depression-like behaviors are more dominant in the immobilization stress-1 and social isolation groups of adult female rats.

Keywords

• immobilization stress,social isolation,depression,anxiety,female rat

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