Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique

Yıl 2024, , 20 - 27, 24.04.2024

Şeyma Korkmaz , Hafizullah Sharifi , Ayşen Demir Mülazımoğlu

https://doi.org/10.35238/sufefd.1456420

Öz

In the present work, electrochemical and spectroelectrochemical behaviors of Serotonin (5-HT) were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Cyclic voltammetric experiments of 5-HT on graphene/coal tar pitch/pencil graphite electrode (GR/CTP/PGE) were carried out between 0.0 V and 1.8 V potential range at a scan rate of 100 mV s-1 with 20 cycles in non-aqueous media. Surface characterizations were performed using CV, EIS and scanning electron microscope (SEM). Effect of different pH values was investigated by square wave voltammetry (SWV) for determination of 5-HT. Optimization of accumulation time was determined using square wave adsorptive stripping voltammetry (SWAdSV) within potential range of -0.2 to +0.6 V. 5-HT standard solutions changing from 75 µM to 1.0 µM were prepared and the corresponding peak currents were measured. From the obtained data calibration equation was derived Ip = 0.0329C5-HT + 0.1511 with correlation coefficient (R2) 0.9958. LOD was 3.51x10-7 M and LOQ was 1.05x10-6 M.

Anahtar Kelimeler

Serotonin, Cyclic Voltammetry, Sensor Electrode, Square Wave Adsorptive Stripping Voltammetry

Destekleyen Kurum

Research Foundation of Necmettin Erbakan University

Proje Numarası

BAP-23DR10002

Teşekkür

We extend our gratitude to the Research Foundation of Necmettin Erbakan University, Konya-TURKEY (BAP-23DR10002) for their financial support of this project.

Kare Dalga Adsorptif Sıyırma Voltametrisi Tekniği Kullanılarak Grafen/Katran/Kalem Grafit Sensör Elektrot ile Serotonin’in Tayini ve Elektrokimyasal Özellikleri

Öz

Bu çalışmada Serotonin’in (5-HT) elektrokimyasal ve spektroelektrokimyasal davranışları dönüşümlü voltametri (CV) ve elektrokimyasal impedans spektroskopisi (EIS) teknikleriyle incelenmiştir. Grafen/katran/kalem grafit elektrot (GR/CTP/PGE) üzerinde 5-HT’nin CV deneyleri, 0,0 V ile 1,8 V potansiyel aralığında sulu olmayan ortamda Ag/Ag+/(10 mM AgNO3) referans elektrotu kullanılarak 20 döngü ile 100 mV s-1 tarama hızında gerçekleştirildi. Yüzey karakterizasyonları CV, EIS ve taramalı elektron mikroskobu (SEM) kullanılarak yapıldı. 5-HT’nin tayininde farklı pH değerlerinin etkisi kare dalga voltametri (SWV) tekniği ile araştırıldı. Biriktirme süresinin optimizasyonu, -0,2 ile +0,6 V potansiyel aralığında kare dalga adsorptif sıyırma voltametrisi (SWAdSV) kullanılarak belirlendi. 75 µM’den 1,0 µM’ye kadar değişen 5-HT standart çözeltileri hazırlandı ve karşılık gelen pik akımları ölçüldü. Elde edilen verilerden kalibrasyon denklemi Ip = 0,0329C5-HT + 0,1511 ve korelasyon katsayısı (R2) 0,9958 olarak hesaplandı. LOD 3,51x10-7 M ve LOQ 1,05x10-6 M olarak belirlendi.

Anahtar Kelimeler

Serotonin, Dönüşümlü Voltametri, Sensör Elektrot, Kare Dalga Adsorptif Sıyırma Voltametrisi

Proje Numarası

BAP-23DR10002

Kaynakça

• Albayrak, I., Erkal, A., Yavuz, S., Kariper, I. A., Mülazımoğlu, I. E., & Üstündağ, Z. (2017). Isophtalic acid terminated graphene oxide modified glassy carbon nanosensor electrode: Cd and Bi analysis in tap water and milk samples. International Journal of Food Properties, 20(7), 1558-1568. https://doi.org/10.1080/10942912.2016.1213743
• Barnett, N. W., Hindson, B. J., & Lewis, S. W. (1998). Determination of 5-hydroxytryptamine (serotonin) and related indoles by flow injection analysis with acidic potassium permanganate chemiluminescence detection. Analytica Chimica Acta, 362(2-3), 131-139. https://doi.org/10.1016/S0003-2670(98)00058-0
• Demir Mülazımoğlu, A., Yılmaz, E., & Mülazımoğlu, I. E. (2012). Dithiooxamide Modified Glassy Carbon Electrode for the Studies of Non-Aqueous Media: Electrochemical Behaviors of Quercetin on the Electrode Surface. Sensors, 12(4), 3916-3928. https://doi.org/10.3390/s120403916
• Demir Mülazımoğlu, A. , & Mülazımoğlu, I. E. (2013). Electrochemical Behaviors of 2-Amino-3-Hydroxypyridine onto the Glassy Carbon Sensor Electrode: Simultaneous and Independent Determinations of Quercetin, Galangin, 3-Hydroxyflavone, and Chrysin. Food Analytical Methods, 6(3), 845-853. https://doi.org/10.1007/s12161-012-9492-5
• Durmuş, N., & Mülazımoğlu, I. E. (2023). Quantitative Determination of Quercetin in Black Tea and Beet Juice using SWAdSV onto The Pencil Graphite Electrode Surface. Analytical & Bioanalytical Electrochemistry, 15(11), 924-937. https://doi.org/10.22034/abec.2023.709094
• Islamoglu, N., Mülazımoglu, I. E., & Mülazımoğlu, A. D. (2023). Sensitive and selective determination of paracetamol in antipyretic children's syrup with a polyglycine modified glassy carbon electrode. Analytical Methods. https://doi.org/10.1039/d3ay00789h
• Kema, I. P., de Vries, E. G. E., & Muskiet, F. A. J. (2000). Clinical chemistry of serotonin and metabolites. Journal of Chromatography B, 747(1-2), 33-48. https://doi.org/10.1016/S0378-4347(00)00341-8
• Kim, S. K., Kim, D., & Jeon, S. (2012). Electrochemical determination of serotonin on glassy carbon electrode modified with various graphene nanomaterials. Sensors and Actuators B-Chemical, 174, 285-291. https://doi.org/10.1016/j.snb.2012.08.034
• Koluaçık, E., Karabiberoglu, S. U., & Dursun, Z. (2018). Electrochemical Determination of Serotonin Using Pre-treated Multi-walled Carbon Nanotube-polyaniline Composite Electrode. Electroanalysis, 30(12), 2977-2987. https://doi.org/10.1002/elan.201800588
• Korkmaz, Ş., Bosnali, W., Mülazımoğlu, I. E., & Mülazımoğlu, A. D. (2023). Voltammetric Determination of Acrylamide Using Coal Tar Pitch Modified Pencil Graphite Electrode by SWV. Food Analytical Methods, 16(11-12), 1738-1745. https://doi.org/10.1007/s12161-023-02540-2
• Leau, S. A., Lete, C., Matei, C., & Lupu, S. (2023). Electrochemical Sensing Platform Based on Metal Nanoparticles for Epinephrine and Serotonin. Biosensors-Basel, 13(8). https://doi.org/10.3390/bios13080781
• Li, J., & Lin, X. Q. (2007). Simultaneous determination of dopamine and serotonin on gold nanocluster/overoxidized-polypyrrole composite modified glassy carbon electrode. Sensors and Actuators B-Chemical, 124(2), 486-493. https://doi.org/10.1016/j.snb.2007.01.021
• Motsaathebe, P. C., & Fayemi, O. E. (2021). Serotonin electrochemical detection in tomatoes at MWCNT-AONP nanocomposite modified electrode. Materials Research Express, 8(11). https://doi.org/10.1088/2053-1591/ac31fd Mülazımoğlu, I. E., & Yılmaz, E. (2010). Quantitative determination of phenol in natural decayed leaves using procaine modified carbon paste electrode surface by cyclic voltammetry. Desalination, 256(1-3), 64-69. https://doi.org/10.1016/j.desal.2010.02.014
• Mumtaz, M., Narasimhachari, N., Friedel, R. O., Pandey, G. N., & Davis, J. M. (1982). Evaluation of Fluorometric Assay-Methods for Serotonin in Platelets, Plasma and Whole-Blood Samples by Comparison with Gc-Ms-Sim Technique. Research Communications in Chemical Pathology and Pharmacology, 36(1), 45-60. <Go to ISI>://WOS:A1982NL87400004
• Özcan, A., & Ilkbas, S. (2015). Poly(pyrrole-3-carboxylic acid)-modified pencil graphite electrode for the determination of serotonin in biological samples by adsorptive stripping voltammetry. Sensors and Actuators B-Chemical, 215, 518-524. https://doi.org/10.1016/j.snb.2015.03.100
• Szeitz, A., & Bandiera, S. M. (2018). Analysis and measurement of serotonin. Biomedical Chromatography, 32(1). https://doi.org/10.1002/bmc.4135
• Üstündağ, I., & Erkal, A. (2017). Determination of Dopamine in the Presence of Ascorbic Acid on Digitonin-Doped Coal Tar Pitch Carbonaceous Electrode. Sensors and Materials, 29(1), 85-94. https://doi.org/10.18494/Sam.2017.1416
• Yilmaz, C., Tas, N. G., Kocadagli, T., & Gökmen, V. (2019). Determination of serotonin in nuts and nut containing products by liquid chromatography tandem mass spectrometry. Food Chemistry, 272, 347-353. https://doi.org/10.1016/j.foodchem.2018.08.064