Can Lathyrus czeczottianus Bässler Be a New Source of Natural Antimutagenic Agents in Pharmacology? Evaluation from Mutagenic/Antimutagenic and Antimicrobial Perspectives

Yıl 2024, , 35 - 42, 01.10.2024

Mustafa Kul , Ahmet Uysal

https://doi.org/10.35238/sufefd.1475894

Öz

In this study, the mutagenicity/antimutagenicity and antimicrobial properties of methanol and water extracts of the aerial parts of the Lathyrus czeczottianus plant were investigated. After toxic dose determinations of the extracts were made, their mutagenic properties were evaluated by the Ames test (Salmonella/microsome). Mutagenicity studies with Salmonella typhimurium TA98 and TA100 strains were performed in the presence and absence of metabolic activation. Methanol and water extracts did not show any mutagenic effects at doses of 10000, 5000 and 1000 µg/plate. Antimutagenic inhibition rates against well-known mutagenic substances were evaluated. Accordingly, methanol and water extracts inhibited the effect of 2 aminofluorene at the highest doses in the presence of the S9 mixture by 73% and 85%, respectively, especially for the TA98 strain. In addition, methanol extract improved the effect of 2 aminoanthracene up to 78% in the presence of S9 for the TA100 strain and showed strong antimutagenicity. Antimicrobial activity studies were tested using the broth microdilution method against eight standard microorganisms and 14 methicillin-resistant Staphylococcus aureus isolates. Methanol and water extracts revealed low antimicrobial activity against the tested microorganisms at doses of 12.5 and 6.25 mg/ml. It was thought that L. czeczottianus extracts could be used as a natural chemo preventive agent against chemicals. This study is the first report on genotoxicity and antimicrobial properties on L. czeczottianus.

Anahtar Kelimeler

Antimicrobial activity, Antimutagenicity, Ames test, Lathyrus czeczottianus, Mutagenicity

Lathyrus czeczottianus Bässler Farmakolojide Doğal Antimutajenik Ajanların Yeni Kaynağı Olabilir mi? Mutajenik/Antimutajenik ve Antimikrobiyal Açıdan Değerlendirme

Öz

Bu çalışmada, Lathyrus czeczottianus bitkisinin toprak üstü kısımlarına ait metanol ve su özütlerinin mutajenite/antimutajenite ve antimikrobiyal özellikleri araştırılmıştır. Özütlerin toksik doz belirlemeleri yapıldıktan sonra, Ames testi (Salmonella/mikrozom) ile mutajenik özellikleri değerlendirilmiştir. Salmonella typhimurium TA98 ve TA100 suşları ile yapılan mutajenite çalışmaları metabolik aktivasyon varlığı ve yokluğunda gerçekleştirilmiştir. Metanol ve su öztüleri 10000, 5000 ve 1000 µg/plak dozlarda herhangi mutajenik etki göstermemiştir. Bilinen mutajenik maddelere karşı antimutajenik inhibisyon oranları değerlendirilmiştir. Buna göre metanol ve su özütleri özellikle TA98 suşu için 2 aminoflorenin S9 karışımı varlığında etkisini sırasıyla %73 ve %85 oranlarda en yüksek dozlarda inhibe etmiştir. Ayrıca metanol özütü TA100 suşu için S9 varlığında 2 aminoantrasenin etkisini %78’lere varan düzeyde iyileştirmiş ve güçlü antimutajenite göstermiştir. Antimikrobiyal aktivite çalışmaları ise sekiz standart mikroorganizma ve 14 metisilin dirençli Staphylococcus aureus izolatına karşı sıvı mikro dilüsyon yöntemiyle test edilmiştir. Metanol ve su özütleri test edilen mikroorganizmalara karşı 12.5 ve 6.25 mg/ml dozlarda düşük antimikrobiyal aktivite ortaya koymuştur. L. czeczottianus özütlerinin kimyasallara karşı kemopreventif doğal bir ajan olarak kullanılabileceği düşünülmüştür.

Anahtar Kelimeler

Antimikrobiyal aktivite, Antimutajenite, Ames test, Lathyrus czeczottianus, Mutajenite

Teşekkür

Bitkinin toplanmasında ve özütlerin hazırlanmasında yardımlarını esirgemeyen Prof. Dr. Gökhan Zengin (Selçuk Üniversitesi) ve Dr. Ramazan Ceylan (Sabancı Üniversitesi)’a teşekkürlerimizi sunarız.

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