Determination of Genetic Diversity among Phaseolus vulgaris (Leguminosae) Populations Collected from Konya Region by SDS-PAGE

Yıl 2010, Cilt: 2 Sayı: 35, 59 - 64, 01.12.2010

Elif Gülbahçe , Buğra Özkubat Emine Arslan

Öz

This study aimed to determine the genetic diversity among 17 Phaseolus vulgaris populations collected from districts of Konya by SDS-PAGE. Seed storage protein profiles were scored visually as present (1) or absent (0). In all, 37 polypeptide bands of different sizes ranging from 14.4 to ~135 kDa were observed in the 17 Phaseolus vulgaris populations. Cluster analysis was performed using the UPGMA in Bio 1 D ++ computer program. Similarity among genotypes was estimated basing on Nei’s homology and a dendrogram was obtained. According to dendrogram, 17 populations belonging to Phaseolus vulgaris were similar in rates ranging from 68 to 100 %. While the highest similarity was found between Kanada (Çumra) and Sarnıç (Altınekin) genotypes, the most distant genotype was Sarhoş (Washington) (Derbent).

Anahtar Kelimeler

Phaseolus vulgaris, Genetic diversity, SDS-PAGE, Seed storage protein

Konya Bölgesinden Toplanan Phaseolus vulgaris (Leguminosae) Populasyonları Arasındaki Genetik Çeşitliliğin SDS-PAGE Yöntemi ile Belirlenmesi

Öz

Bu çalışmada Konya’nın ilçelerinden toplanan 17 Phaseolus vulgaris populasyonu arasındaki genetik çeşitliliğin SDS-PAGE ile belirlenmesi amaçlanmıştır. Tohum depo protein profilleri görsel şekilde var (1) ya da yok (0) olarak skorlanmıştır. On yedi Phaseolus vulgaris populasyonunda, 14.4 ve ~135 kDa arasında değişen farklı büyüklüklerde toplamda 37 polipeptit bant gözlenmiştir. Bio1D++ bilgisayar programında UPGMA kullanılarak kümeleme analizi yapılmıştır. Genotipler arasındaki benzerlik Nei’nin homolojisine dayanarak tahmin edilmiş ve bir dendrogram elde edilmiştir. Dendrograma göre Phaseolus vulgaris’e ait 17 populasyon %68-100 arasında değişen oranlarda benzer bulunmuştur. En yüksek benzerlik Kanada (Çumra) ve Sarnıç (Altınekin) genotipleri arasında bulunurken, en uzak genotip Sarhoş (Washington) (Derbent) olmuştur.

Anahtar Kelimeler

Phaseolus vulgaris, Genetik çeşitlilik, SDS-PAGE, Tohum depo protein

Kaynakça

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