Yıl 2017, Cilt 27 , Sayı 4, Sayfalar 334 - 341 2017-12-27

ELEKTRO ÇEKİM YÖNTEMİ İLE POLİETİLEN TEREFTALAT DOKU İSKELESİ ÜRETİMİ: KARAKTERİZASYONU VE IN VITRO ORTAMDA HÜCRE ÇOĞALMASINDAKİ POTANSİYELİ
FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO

Şebnem DÜZYER [1]


Lif çapı ve yüzey gözenekliliği hücre tutunması ve çoğalmasında önemli bir rol oynamaktadır. Bu nedenle bu çalışmada, farklı lif çaplarına ve yüzey gözenekliliğine sahip polietilen tereftalat (PET) doku iskeleleri elektro çekim yöntemi ile farklı PET konsantrasyonuna sahip çözeltilerden (ağırlıkça %10,%15 ve %20) üretilmiştir. Ayrıca, üretilen yüzeylerin kalınlığının etkisi incelenmiştir. Doku iskelelerinin yüzeysel ve mekaniksel karakterizasyonu yapılmıştır. Elektro çekim yöntemi ile üretilen liflerin polimer konsantrasyonuna bağlı olarak 0.575 μm’den 2.825 μm’ye değişen aralıkta çaplara sahip olduğu görülmüştür. Temas açısı ölçümleri PET doku iskelelerinin süper hidrofobik yapıya sahip olduğunu göstermiştir. Karakterizasyon sonrası, PET doku iskeleleri üzerine fibroblast hücre ekimleri yapılmış ve farklı elektro çekim parametrelerinin hücre çoğalması üzerine etkisi araştırılmıştır. Fibroblast hücreleri küçük çaplara sahip liflerden oluşan yüzeyler üzerinde daha iyi çoğalmıştır. PET doku iskeleleri üzerine fibroblast hücre çoğalması davranışı incelendikten sonra, üretilmiş olan dokuz yüzeyden en küçük lif çapına sahip olan ve daha iyi mekanik özelliklere sahip olan iki yüzey seçilmiştir. Bu iki yüzey üzerine endotel ve keratinosit hücre ekimleri yapılmıştır. Endotel ve keratinosit hücrelerinin bu iki yüzey üzerindeki hücre tutunması ve çoğalması davranışları incelenmiştir.
Fiber diameter and fiber mat porosity play a key role on cell adhesion and proliferation. Therefore, in this study, poly(ethylene terephthalate) (PET) scaffolds consisting of fibers with different diameters and pore sizes were fabricated from solutions with different concentrations (10, 15 and 20% wt.) by electrospinning. Also, the effect of fiber mat thickness was investigated. The scaffolds were characterized in terms of surface and mechanical properties. The electrospun fibers had diameters ranging from 0.575 to 2.825 μm depending on the polymer concentration. Contact angle values showed that PET scaffolds had super hydrophobic structure. After characterization, fibroblast cells were cultured onto PET scaffolds and influence of different electrospinning parameters on cell proliferation was discussed. Fibroblast cells showed better proliferation on scaffolds consisting of smaller diameters. After evaluation of fibroblast cell proliferation on electrospun PET scaffolds, two different electrospun scaffolds were chosen: 1) scaffold with the smallest fiber diameter and 2) scaffold with better mechanical properties. Endothelial and keratinocyte cells were cultured on those two electrospun scaffolds. Cell adhesion and proliferation behavior of endothelial and keratinocyte cells on those scaffolds were investigated.
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Bölüm Makaleler
Yazarlar

Yazar: Şebnem DÜZYER (Sorumlu Yazar)

Tarihler

Yayımlanma Tarihi : 27 Aralık 2017

Bibtex @araştırma makalesi { tekstilvekonfeksiyon372022, journal = {Textile and Apparel}, issn = {1300-3356}, eissn = {2602-3075}, address = {Ege Üniversitesi Tekstil ve Konfeksiyon Araştırma Uygulama Merkezi 35100 Bornova - İZMİR}, publisher = {Ege Üniversitesi}, year = {2017}, volume = {27}, pages = {334 - 341}, doi = {}, title = {FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO}, key = {cite}, author = {DÜZYER, Şebnem} }
APA DÜZYER, Ş . (2017). FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Textile and Apparel , 27 (4) , 334-341 . Retrieved from https://dergipark.org.tr/tr/pub/tekstilvekonfeksiyon/issue/33462/372022
MLA DÜZYER, Ş . "FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO". Textile and Apparel 27 (2017 ): 334-341 <https://dergipark.org.tr/tr/pub/tekstilvekonfeksiyon/issue/33462/372022>
Chicago DÜZYER, Ş . "FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO". Textile and Apparel 27 (2017 ): 334-341
RIS TY - JOUR T1 - FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO AU - Şebnem DÜZYER Y1 - 2017 PY - 2017 N1 - DO - T2 - Textile and Apparel JF - Journal JO - JOR SP - 334 EP - 341 VL - 27 IS - 4 SN - 1300-3356-2602-3075 M3 - UR - Y2 - 2017 ER -
EndNote %0 Tekstil ve Konfeksiyon FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO %A Şebnem DÜZYER %T FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO %D 2017 %J Textile and Apparel %P 1300-3356-2602-3075 %V 27 %N 4 %R %U
ISNAD DÜZYER, Şebnem . "FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO". Textile and Apparel 27 / 4 (Aralık 2017): 334-341 .
AMA DÜZYER Ş . FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Textile and Apparel. 2017; 27(4): 334-341.
Vancouver DÜZYER Ş . FABRICATION OF ELECTROSPUN POLY (ETHYLENE TEREPHTHALATE) SCAFFOLDS: CHARACTERIZATION AND THEIR POTENTIAL ON CELL PROLIFERATION IN VITRO. Textile and Apparel. 2017; 27(4): 341-334.