BIOLOGICAL CONTROL OF AFLATOXIGENIC FUNGI ON PEANUT: FOR THE PRE-HARVEST APPROACH
Year 2019,
, 21 - 27, 14.06.2019
İsılay Lavkor
,
Halis Arıoglu
İsıl Var
Sevcan Oztemız
Abstract
This study was carried out to determine the efficacy of different applications of a biopesticide for reduction of aflatoxin contamination in peanut. The biopesticide, afla-guard, delivers a nontoxigenic Aspergillus flavus to the field where it competes with naturally occurring toxigenic fungus. Biocontrol treatments included: (ı) soil application during sowing, (ıı) multiple application during sowing and 40 days after planting, (ııı) foliar application at 60 days after planting (ıv) control (untreated plots). Biopesiticide was applied to peanut plots in 2015 and 2016 in Randomized Complete Block Design with four replications. Peanuts were collected from control and treated plots at harvest-drying-pre-storage periods and analysed for aflatoxins. Aflatoxin concentrations were generally quite low in 2015, also the aflatoxin concentration in treated samples (from 0.04 to 0.71 μg/kg) was reduced by 97.38 to 99.82% compared with controls (from 21.84 to 27.12 μg/kg). In 2016, reductions were also noted for all biocontrol treatments (from 89.07 to 92.39%) compared with controls. In conjunction with the reductions in aflatoxin contamination, biocontrol treatments produced significant reductions with biopesticide in peanut. Therefore, it can be said that a biological control method is a promising approach for controlling aflatoxin.
Supporting Institution
The Scientific Technological Research Council of Turkey (TUBITAK).
Thanks
This study (Project No 115O007) was financial supported
by The Scientific Technological Research Council of
Turkey (TUBITAK).
References
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afla-guard-gr. Afla-Guard® GR
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Turkish State Meteorological Service Adana Regional
Directorship, 2015, 2016.
- Arıoglu, H. 2000. Peanuts cultivation breeding. Adana, Turkey:
Cukurova University Faculty of Agriculture publishing.
- Arzandeh, S. and S. Jinap. 2011. Effect of initial aflatoxin
concentration, heating time and roasting temperature on
aflatoxin reduction in contaminated peanuts and process
optimization using response surface modeling. Int. J. Food
Sci. Technol. 46: 485–491.
- Atehnkeng, J., P.S. Ojiambob, P.J. Cotty and R.
Bandyopadhyay. 2014. Field efficacy of a mixture of
atoxigenic Aspergillus flavus Link: FR vegetative
compatibility groups in preventing aflatoxin contamination
in maize (Zea mays L.). Biol. Control, 72: 62-70.
- Chepsergon, J., E.K. Kiprop, L.A. Mwamburi. Biocontrol of
aflatoxins in corn using atoxigenic Aspergillus flavus.
Review International Journal of Science and Research.
12:1954-1958.
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1989. Environmental conditions required to induce
preharvest aflatoxin contamination of groundnuts: Summary
of six years’ research. In: Aflatoxin contamination of
groundnut: Proceedings of the international workshop, ed.
Hall SD., 279-287, ICRISAT Center, Patancheru, AP, India.
- Denizel, T. and O. Kosker. 1972. A mycological survey of
various kinds of nuts commercially available in the UK with
reference to mycotoxins. University of Ankara, Turkey:
Yearbook of the Faculty of Agriculture.
- Dorner, J.W., R.J. Cole and P.D. Blankenship. 1992. Use of a
biocompetitive agent to control preharvest aflatoxin in
drought stressed peanuts. J. Food Prot. 55: 888-892.
- Dorner, J.W., R.J. Cole and P.D. Blankenship. 1998. Effect of
inoculum rate of biological control agents on preharvest
aflatoxin contamination of peanuts. Biol. Cont. 12: 171-176.
- Dorner, J.W. and R.J. Cole. 2002. Effect of application of
nontoxigenic strains of Aspergillus flavus and A. parasiticus
on subsequent aflatoxin contamination of peanuts in storage.
J. Stored Prod. Res. 38: 329-339.
- Dorner, J.W., R.J. Cole, W.J. Connick, D.J. Daigle, M.R.
Mcguire and B.S. Shashac. 2003. Evaluation of biological
control formulations to reduce aflatoxin contamination in
peanuts. Biol. Cont. 26: 318-324.
- Dorner, J.W. 2004. Combined effects of biological control
formulations, cultivars, and fungicides on preharvest
colonization and aflatoxin contamination of peanuts by
Aspergillus species. Peanut Sci. 31: 79–86.
- Dorner, J.W. 2005. Biological control of aflatoxin crop
contamination. In: Aflatoxin and food safety, ed. Abbas
H.K., 333–352, Taylor and Francis, New York, USA.
- Dorner, J.W. and M.C. Lamb. 2006. Development and
commercial use of Afla-Guard(®) an aflatoxin biocontrol
agent. Mycotoxin Res. 22: 33-38.
- Dorner, J.W. and B.W. Horn. 2007. Separate and combined
applications of nontoxigenic Aspergillus flavus and A.
parasiticus for biocontrol of aflatoxin in peanuts.
Mycopathologia 163:215–223.
- Dorner, J.W. 2008. Management and prevention of mycotoxins
in peanuts. Food Additives and Contaminants. 25: 203–208.
- Dorner, J.W. 2009. Development of biocontrol technology to
manage aflatoxin contamination in peanuts. Peanut Sci. 36:
60-67.
- Dorner, J.W. 2010. Efficacy of a biopesticide for control of
aflatoxins in corn. J. Food Prot. 73: 495–499.
- Gomez, A.K. and A.A. Gomez. 1983. Statistical Procedures for
Agricultural Research. New York: USA: Wiley.
- IARC. 1993. Some naturally occurring substances: food items
and constituents, heterocyclic aromatic amines and
mycotoxins. International Agency for Research on Cancer.
http://monographs.iarc.fr/ENG/Monographs/vol56/mono56.p
df, (Accessed 21 June 2017)
- Isakeit, T., S. Murray and K. Mayfield. 2010. Efficacy of Afla-
Guard (Aspergillus flavus NRRL 21882) to control aflatoxin
on corn in Burleson County, Texas, 2009. Plant Disease
Management Reports 4: FC081. www.
plantmanagementnetwork.org/pub/trial/PDMR/reports
/2010/FC081.pdf, (Accessed January 20, 2018)
- Kumar, V., M.S. Basu and T.P. Rajendran. 2008. Mycotoxin
research and mycoflora in some commercially important
agricultural commodities. Crop Prot. 27: 891–905.
- Lavkor, I. 2013. Control of diseases and aflatoxin occurrences
with proper cultural and disease management practices in
peanut growing. Ph.D. Cukurova University, Adana, p. 300.
- Lavkor, I. and M. Bicici. 2015. Aflatoxin occurrence in peanuts
grown in Osmaniye at harvest, post-harvest, drying and prestorage
periods. J. Agric. Sci. 21: 394-405.
- Lavkor, I., I. Var, S. Oztemiz and M. Almatar. 2017. First report
of mycotoxins in second peanuts crop in Adana and
Osmaniye at harvest, drying, pre-storage and storage periods.
In: Aflatoxin-Control, Analysis, Detection and Health Risks,
ed. Abdulra’uf L B, 193-208. InTech Open Science, Rijeka,
Croatia.
- Makun, H.A., S.T. Anjorin, B. Moronfoye, F. Adejo, O.A.
Afolabi, G. Fagbayibo, B.O. Balogun, and A.A. Surajudeen.
2010. Fungal and aflatoxin contamination of some human
food commodities in Nigeria. Afr. J. Food Sci. 4: 127-135.
- Mutegi, C.K., H.K. Ngugi, S.L. Hendiks and R.B. Jones. 2012.
Factors associated with the incidence of Aspergillus section
Flavi and aflatoxins contamination of peanuts in Busia and
Homa bay districts of Western Kenya. Plant Pathol. J. 61:
1143-1153.
- Pitt, J.I. and Hocking, A.D. 2006. Mycotoxins in Australia:
biocontrol of aflatoxin in peanuts. Mycopathologia, 162:
233-243
- Richard, J.L. 2007. Some major mycotoxins and their
mycotoxicoses- an overview. Int. J. Food Microbiol. 119: 3-
10.
- Russell, R., M. Paterson and N. Lima. 2010. How the climate
change affect mycotoxins in food? Food Res. Int. 43: 1902-
1914.
- Sigh, P., J. March and P.J. Cotty. Aspergillus texensis: a novel
aflatoxin producer with S morphology from the United
States. Toxins 2018, 10, 513.
- Waliyar, F., S.V. Reddy and P.L. Kumar. 2009. Review of
immunological methods for the quantification of aflatoxins
in peanut and other foods. Peanut Sci. 36: 54-59.
- Wu, F. and P. Khlangwiset. 2010. Health economic impacts and
cost-effectiveness of aflatoxin reduction strategies in Africa:
Case studies in biocontrol and postharvest interventions.
Food Addit. Contam. A. 27: 496-509.
- Yu, J., P.K. Chang, K.C. Ehrlich, J.W. Cary, D. Bhatnagar, T.E.
Cleveland, G.A. Payne, J.E. Linz, J.E., C.P. Woloshuk and
J.W. Bennett. 2004. Clustered pathway genes in aflatoxin
biosynthesis. Appl. Environ. Microbiol. 70: 1253-1262.
- Yin, Y., L. Yan, J. Jiang and Z. Ma.2008. Biological control of
aflatoxin contamination of crops. Journal of Zhejiang
University, Science B.9:787-792.
Year 2019,
, 21 - 27, 14.06.2019
İsılay Lavkor
,
Halis Arıoglu
İsıl Var
Sevcan Oztemız
References
- Anonymous, 2014. http://www.syngenta-us.com/currentlabel/
afla-guard-gr. Afla-Guard® GR
- Anonymous, 2017. The meteorological data for Adana. The
Turkish State Meteorological Service Adana Regional
Directorship, 2015, 2016.
- Arıoglu, H. 2000. Peanuts cultivation breeding. Adana, Turkey:
Cukurova University Faculty of Agriculture publishing.
- Arzandeh, S. and S. Jinap. 2011. Effect of initial aflatoxin
concentration, heating time and roasting temperature on
aflatoxin reduction in contaminated peanuts and process
optimization using response surface modeling. Int. J. Food
Sci. Technol. 46: 485–491.
- Atehnkeng, J., P.S. Ojiambob, P.J. Cotty and R.
Bandyopadhyay. 2014. Field efficacy of a mixture of
atoxigenic Aspergillus flavus Link: FR vegetative
compatibility groups in preventing aflatoxin contamination
in maize (Zea mays L.). Biol. Control, 72: 62-70.
- Chepsergon, J., E.K. Kiprop, L.A. Mwamburi. Biocontrol of
aflatoxins in corn using atoxigenic Aspergillus flavus.
Review International Journal of Science and Research.
12:1954-1958.
- Cole, R.J., T.H. Sanders, J.W. Dorner and P.D. Blankenship.
1989. Environmental conditions required to induce
preharvest aflatoxin contamination of groundnuts: Summary
of six years’ research. In: Aflatoxin contamination of
groundnut: Proceedings of the international workshop, ed.
Hall SD., 279-287, ICRISAT Center, Patancheru, AP, India.
- Denizel, T. and O. Kosker. 1972. A mycological survey of
various kinds of nuts commercially available in the UK with
reference to mycotoxins. University of Ankara, Turkey:
Yearbook of the Faculty of Agriculture.
- Dorner, J.W., R.J. Cole and P.D. Blankenship. 1992. Use of a
biocompetitive agent to control preharvest aflatoxin in
drought stressed peanuts. J. Food Prot. 55: 888-892.
- Dorner, J.W., R.J. Cole and P.D. Blankenship. 1998. Effect of
inoculum rate of biological control agents on preharvest
aflatoxin contamination of peanuts. Biol. Cont. 12: 171-176.
- Dorner, J.W. and R.J. Cole. 2002. Effect of application of
nontoxigenic strains of Aspergillus flavus and A. parasiticus
on subsequent aflatoxin contamination of peanuts in storage.
J. Stored Prod. Res. 38: 329-339.
- Dorner, J.W., R.J. Cole, W.J. Connick, D.J. Daigle, M.R.
Mcguire and B.S. Shashac. 2003. Evaluation of biological
control formulations to reduce aflatoxin contamination in
peanuts. Biol. Cont. 26: 318-324.
- Dorner, J.W. 2004. Combined effects of biological control
formulations, cultivars, and fungicides on preharvest
colonization and aflatoxin contamination of peanuts by
Aspergillus species. Peanut Sci. 31: 79–86.
- Dorner, J.W. 2005. Biological control of aflatoxin crop
contamination. In: Aflatoxin and food safety, ed. Abbas
H.K., 333–352, Taylor and Francis, New York, USA.
- Dorner, J.W. and M.C. Lamb. 2006. Development and
commercial use of Afla-Guard(®) an aflatoxin biocontrol
agent. Mycotoxin Res. 22: 33-38.
- Dorner, J.W. and B.W. Horn. 2007. Separate and combined
applications of nontoxigenic Aspergillus flavus and A.
parasiticus for biocontrol of aflatoxin in peanuts.
Mycopathologia 163:215–223.
- Dorner, J.W. 2008. Management and prevention of mycotoxins
in peanuts. Food Additives and Contaminants. 25: 203–208.
- Dorner, J.W. 2009. Development of biocontrol technology to
manage aflatoxin contamination in peanuts. Peanut Sci. 36:
60-67.
- Dorner, J.W. 2010. Efficacy of a biopesticide for control of
aflatoxins in corn. J. Food Prot. 73: 495–499.
- Gomez, A.K. and A.A. Gomez. 1983. Statistical Procedures for
Agricultural Research. New York: USA: Wiley.
- IARC. 1993. Some naturally occurring substances: food items
and constituents, heterocyclic aromatic amines and
mycotoxins. International Agency for Research on Cancer.
http://monographs.iarc.fr/ENG/Monographs/vol56/mono56.p
df, (Accessed 21 June 2017)
- Isakeit, T., S. Murray and K. Mayfield. 2010. Efficacy of Afla-
Guard (Aspergillus flavus NRRL 21882) to control aflatoxin
on corn in Burleson County, Texas, 2009. Plant Disease
Management Reports 4: FC081. www.
plantmanagementnetwork.org/pub/trial/PDMR/reports
/2010/FC081.pdf, (Accessed January 20, 2018)
- Kumar, V., M.S. Basu and T.P. Rajendran. 2008. Mycotoxin
research and mycoflora in some commercially important
agricultural commodities. Crop Prot. 27: 891–905.
- Lavkor, I. 2013. Control of diseases and aflatoxin occurrences
with proper cultural and disease management practices in
peanut growing. Ph.D. Cukurova University, Adana, p. 300.
- Lavkor, I. and M. Bicici. 2015. Aflatoxin occurrence in peanuts
grown in Osmaniye at harvest, post-harvest, drying and prestorage
periods. J. Agric. Sci. 21: 394-405.
- Lavkor, I., I. Var, S. Oztemiz and M. Almatar. 2017. First report
of mycotoxins in second peanuts crop in Adana and
Osmaniye at harvest, drying, pre-storage and storage periods.
In: Aflatoxin-Control, Analysis, Detection and Health Risks,
ed. Abdulra’uf L B, 193-208. InTech Open Science, Rijeka,
Croatia.
- Makun, H.A., S.T. Anjorin, B. Moronfoye, F. Adejo, O.A.
Afolabi, G. Fagbayibo, B.O. Balogun, and A.A. Surajudeen.
2010. Fungal and aflatoxin contamination of some human
food commodities in Nigeria. Afr. J. Food Sci. 4: 127-135.
- Mutegi, C.K., H.K. Ngugi, S.L. Hendiks and R.B. Jones. 2012.
Factors associated with the incidence of Aspergillus section
Flavi and aflatoxins contamination of peanuts in Busia and
Homa bay districts of Western Kenya. Plant Pathol. J. 61:
1143-1153.
- Pitt, J.I. and Hocking, A.D. 2006. Mycotoxins in Australia:
biocontrol of aflatoxin in peanuts. Mycopathologia, 162:
233-243
- Richard, J.L. 2007. Some major mycotoxins and their
mycotoxicoses- an overview. Int. J. Food Microbiol. 119: 3-
10.
- Russell, R., M. Paterson and N. Lima. 2010. How the climate
change affect mycotoxins in food? Food Res. Int. 43: 1902-
1914.
- Sigh, P., J. March and P.J. Cotty. Aspergillus texensis: a novel
aflatoxin producer with S morphology from the United
States. Toxins 2018, 10, 513.
- Waliyar, F., S.V. Reddy and P.L. Kumar. 2009. Review of
immunological methods for the quantification of aflatoxins
in peanut and other foods. Peanut Sci. 36: 54-59.
- Wu, F. and P. Khlangwiset. 2010. Health economic impacts and
cost-effectiveness of aflatoxin reduction strategies in Africa:
Case studies in biocontrol and postharvest interventions.
Food Addit. Contam. A. 27: 496-509.
- Yu, J., P.K. Chang, K.C. Ehrlich, J.W. Cary, D. Bhatnagar, T.E.
Cleveland, G.A. Payne, J.E. Linz, J.E., C.P. Woloshuk and
J.W. Bennett. 2004. Clustered pathway genes in aflatoxin
biosynthesis. Appl. Environ. Microbiol. 70: 1253-1262.
- Yin, Y., L. Yan, J. Jiang and Z. Ma.2008. Biological control of
aflatoxin contamination of crops. Journal of Zhejiang
University, Science B.9:787-792.