Alterations in Thiol–Disulfide Homeostasis and Oxidative Stress Markers in Cows with Clinical and Subclinical Mastitis
Abstract
Objective: This study aimed to evaluate alterations in oxidative stress markers and thiol–disulfide homeostasis in cows with clinical and subclinical mastitis. Materials and Methods: A total of 40 cows were included and divided into five groups: clinical mastitis (n = 8), subclinical mastitis (+1, +2, +3; n = 8 for each group), and a healthy control group (n = 8). Blood samples were analyzed for glutathione (GSH), malondialdehyde (MDA), total thiol (TT), native thiol (NT), disulfide levels, and the ratios of disulfide/TT, disulfide/NT, and NT/TT. Results: Compared with the control group, cows with clinical and subclinical mastitis showed significantly lower GSH, TT, and NT levels (P < 0.001), whereas MDA, disulfide concentrations, and disulfide-based ratios were markedly increased, particularly in the clinical mastitis group (P < 0.001). Progressive alterations in oxidative stress markers were observed with increasing severity of subclinical mastitis (P < 0.05). Correlation analyses revealed strong and significant associations between thiol–disulfide homeostasis parameters and oxidative stress markers (P < 0.001). Conclusion: In conclusion, thiol–disulfide homeostasis may serve as a complementary oxidative stress–based biomarker system for the evaluation of both clinical and subclinical mastitis.
Ethical Statement
This study was approved by the Kafkas University Local Ethics Committee for Animal Experiments (KAU-HADYEK/2025-04).
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Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Obstetrics and Gynecology |
| Journal Section | Research Article |
| Authors | |
| Submission Date | January 19, 2026 |
| Acceptance Date | February 16, 2026 |
| Publication Date | March 29, 2026 |
| DOI | https://doi.org/10.47748/tjvr.1866616 |
| IZ | https://izlik.org/JA73RZ58FP |
| Published in Issue | Year 2026 Volume: 10 Issue: 1 |
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Prof. Dr. İsmail Hakkı Ekin, 1971 Yılında Siirt'te doğdu. İlk ve ortaöğrenimini Van'da tamamladı. 1989 yılında Van Teknik Lisesi Motor Bölümünü birincilikle bitirdi. 1990 yılında girdiği Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesinden 1995 yılında mezun oldu. Aynı yıl kısa dönem askerlik görevini yerine getirerek, Temmuz 1996’da terhis oldu. 1997’de Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi’nde Araştırma Görevliliğine atandı. Van Yüzüncü Yıl Üniversitesi Sağlık Bilimleri Enstitüsü Veteriner Mikrobiyoloji Anabilim Dalında başladığı Yüksek Lisans öğrenimini 1998 yılında, Doktora öğrenimini ise 2004 yılında bitirerek Bilim Doktoru (PhD) unvanını aldı. Dr. Araştırma görevlisi olarak 2 yıl süreyle görev yaptıktan sonra Şubat 2006’da Dr. Öğretim Üyesi (Yrd. Doç. Dr.) kadrosuna atandı. Van Yüzüncü Yıl Üniversitesi Sağlık Bilimleri Enstitüsü Besin Hijyeni ve Teknolojisi Anabilim Dalındaki ikinci Doktora eğitimini de 2022 yılında bitirerek ikinci Bilim Doktoru (PhD) unvanını aldı. 2013 yılında Doçentlik unvanını alan Ekin, 2018 yılında Van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalında Profesörlük kadrosuna atandı. Halen bu birimde Anabilim Dalı Başkanı olarak görevini sürdürmekte olup evli ve üç çocuk babasıdır.
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