Antimicrobial Effects of Chitosan Extracted from Crayfish Shells in Cream Formulations

Year 2024, In Press Articles, 1 - 11

Yavuz Mazlum , Selin Sayın , Betül Aydın , Mehmet Naz , Metin Yazıcı , Şerife Akküçük

https://doi.org/10.52998/trjmms.1471661

Abstract

The objective of the present study was to investigate the antimicrobial properties of a chitosan-based cream. To achieve this, the antimicrobial effects a cream enriched with chitosan were compared with those of a control group. Chitosan, sourced from crayfish obtained frozen from Eğirdir Lake, Eğirdir, İsparta, served as the primary material. The study involved a comparison between control (F1) and treatment (F2) groups. While both cream formulations exhibited bacterial inhibition, only the F1 formulation demonstrated significant reduction in viable microorganisms for C. albicans. The cytotoxicity assessment revealed a concentration-dependent increase in cytotoxic effects of the samples. Notably, the F1 formulation exhibited higher toxicity on healthy cells compared to the F2 formulation. In conclusion, further investigation is necessary to understant the mechanisms underlying their cytotoxic effects and to optimize their formulations to enhance biocompatibility. Moreover, the chitosan-based cream developed in this study demonstrated notable antimicrobial efficacy against the tested bacteria.

Keywords

Chitosan, Crayfish, Antimicrobial effect, Cytotoxicity, Skin health

Ethical Statement

Ethics committee permission is not required for this study.

Supporting Institution

This study was not supported by any organization

Project Number

This study was not supported by any organization. There is no project number.

Kerevit Kabuklarından Elde Edilen Kitosanın Krem Formülasyonlarındaki Antimikrobiyal

Abstract

Bu çalışmanın amacı, kitosan bazlı bir kremnin antimikrobiyal özelliklerinin araştırılmasıdır. Bunun için, kitosanla zenginleştirilmiş bir kremnin antimikrobiyal etkileri kontrol grubununkilerle karşılaştırılmıştır. Ana materyal olarak, Eğirdir gölü'nden dondurulmuş bir şekilde temin edilen kerevitlerden elde edilen kitosan kullanıldı. Çalışma, kontrol (F1) ve muamele (F2) grupları arasında bir karşılaştırmayı içermektedir. Her iki krem formülasyonu da bakteriyel inhibisyon gösterirken, yalnızca F1 formülasyonu C. albicans için canlı mikroorganizmalarda önemli bir azalmayı gösterdi. Sitotoksisite değerlendirmesi, örneklerin sitotoksik etkilerinde konsantrasyona bağlı bir artışı ortaya koymuştur. Özellikle, F1 formülasyonunun sağlıklı hücreler üzerinde F2 formülasyonuna kıyasla daha yüksek bir toksisite sergilediği belirlendi. Sonuç olarak, sitotoksik etkilerinin mekanizmalarını anlamak ve biyouyumluluğu artırmak için formülasyonlarını optimize etmek için daha fazla araştırmaya ihtiyaç duyulmaktadır. Ayrıca, bu çalışmada geliştirilen kitosan bazlı krem, test edilen bakterilere karşı dikkate değer antimikrobiyal etkinlik göstermiştir.

Keywords

Kitosan, Kerevit, Antimikrobiyal etki, Sitotoksisite, Deri sağlığı

Project Number

This study was not supported by any organization. There is no project number.

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