Su Pirelerinin Kalp Atışları ile Bazı Uyarıcı ve Toksik Kimyasal Çözeltilere Verdikleri Tepkilerin Belirlenmesi

Year 2025, In Press Articles, 1 - 13

Arda Sarp Karademir , Melisa Can , V. Zülal Sönmez , Nüket Sivri

https://doi.org/10.52998/trjmms.1560994

Abstract

Kolay yetiştiriciliği, vücut şeffaflığı ve kimyasal kirliliğe karşı yüksek hassasiyeti sayesinde su pireleri, ekotoksisite çalışmalarında iyi bir model olarak kabul edilmektedir. Bu çalışmada, Daphnia magna'nın kalp atışları, bilinen toksik kimyasallara maruz kaldığında temel davranışsal ve fizyolojik özelliklerini yansıtarak incelenmiştir. Farklı kimyasal özelliklere sahip oldukları bilinen kristal viole, etanol ve formaldehit toksisiteleri nedeniyle seçilmiştir. Su pirelerinde kalp atışlarını değerlendirerek kardiyovasküler performansı ölçmek için yavaş çekim video tabanlı bir yöntem düzenlenmiştir. Kalp atışları, vücut tepkileri ve kalp kasılmaları gibi önemli parametrelere ilişkin veriler video kayıtlarından ve matematiksel hesaplamalardan elde edilmiştir. Çalışmanın sonuçları oldukça dikkat çekicidir. Kristal viole’nin Daphnia magna'nın kalp atışlarını (489±14.19), etanol (450±40.67) ve formaldehitten (445±48.21) daha belirgin bir şekilde artırdığı bulunmuştur. Kontrol grubuyla karşılaştırıldığında, formaldehit su pirelerinde %28.51 oranında bir kalp atış artışına neden olurken, etanol (%30.54) ve kristal violet (%35.89) su pirelerinin kalp atışlarında daha düşük bir artışa yol açmıştır. Bu kardiyovasküler parametreler kullanılarak, uyarıcı ve toksik kimyasal çözeltilerin su pireleri üzerindeki potansiyel etkileri kesin bir şekilde ölçülmüştür. Daphnia magna, en güçlü kalp atışlarına sahip olduğu ve ekotoksisite değerlendirmesi için uygun olduğu belirlenmiştir. Üç kimyasalın hepsine kısa süreli maruziyet sonucunda organizmanın kalp atışlarında bir artış gözlemlenmiş, ancak uzun süreli maruziyetlerin toksik etkilere yol açarak ölümlere neden olabileceği tespit edilmiştir.

Keywords

Su piresi, Daphnia magna, Kalp atışları, Kristal viole, Etanol

Determining the Response of Water Fleas to Some of the Excitatory and Toxic Chemical Solutions by Heart Rates

Abstract

Water fleas have become a valuable model for ecotoxicity studies due to their ease of cultivation, transparent bodies, and high sensitivity to chemical pollutants. In this study, heart rates in Daphnia magna were examined reflecting the basic behavioral and physiological characteristics when exposed to known toxic chemicals. Crystal violet, ethanol, and formaldehyde, known for their toxicity, were selected due to their different chemical properties. A slow-motion video-based method was organized to measure cardiovascular performance by evaluating the heart rates in water fleas. Data for important parameters like heart rates, body reactions, and heart contraction were extracted from video recordings and mathematical calculations. The results of study were quite remarkable. It was found that crystal violet increased the heart rates of Daphnia magna (489±14.19) more significantly than ethanol (450±40.67) and formaldehyde (445±48.21). Compared to the control group, formaldehyde caused a 28.51% increase in the heart rates of daphnids, while exposure to ethanol (30.54%) and crystal violet (35.89%) resulted in a lower increase in the heart rates of daphnids. Using these cardiovascular parameters, the potential effects of excitatory and toxic chemical solutions on water fleas were accurately measured. Daphnia magna was identified as having the strongest heart rates and is deemed suitable for ecotoxicity assessment. The result of exposure to all three chemicals was an increase in the organism’s heart rates during short-term exposures, while long-term exposures could lead to toxic effects, causing fatalities.

Keywords

Water flea, Daphnia magna, Heart rates, Crystal violet, Ethanol

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