Abstract

Hypericum cinsi, tıbbi ve kozmetik faydaları olan fitokimyasallar bakımından zengin olması nedeniyle Türkiye florası için önemli bir cinstir. Hypericum bilgehan-bilgilii Basköse & Savran'ın (HEO)’nin uçucu yağ bileşimi ve biyolojik aktiviteleri bu çalışmada ilk kez analiz edilmiştir. Hypericum bilgehan-bilgilii tüm kısımlarının uçucu yağı, Clevenger tipi aparatla hidrodistilasyon yoluyla elde edilmiştir. Kimyasal bileşim, polar olmayan kolon kullanılarak GC-MS ile analiz edilmiştir. α-glukozidaz ve 5-lipoksijenaz inhibitör, sitotoksik ve DPPH radikal süpürücü aktiviteleri araştırılmıştır. Kırk sekiz bileşen tanımlanmış ve tüm bileşenlerin %97,3'ünü temsil etmiştir. İlginç bir şekilde, majör uçucular: 1-(2,4,5-trimetoksifenil)bütan-1-on (%27,7) ve 3-Metil-1-(2,4,6-trihidroksifenil)bütan-1-on (%11,2) Hypericum uçucu yağlarında ilk kez tespit edilmiştir. Yağ, 39 µg/mL'lik bir IC50 değeriyle 5-lipoksijenaz enzimine karşı önemli bir aktivite göstermiştir. HEO'nun sitotoksisite potansiyeli, farklı konsantrasyonlarda, dört hücre hattına karşı araştırılmış ve IC50 değerleri hafif sitotoksisitenin varlığını göstermiştir. Bu sonuçlar, H. bilgehan-bilgilii uçucu yağının biyoaktif bileşenler ve anti-inflamatuar ajanlar için değerli bir kaynak olarak kabul edilebileceğini göstermiştir.

Supporting Institution

No external funding acquired.

Ethical Statement

The manuscript does not include any studies on human or animal topics. Ethics committee approval is not required.

Chemical composition of endemic Hypericum bilgehan-bilgilii Basköse & Savran essential oil and its α-glucosidase antidiabetic, anti-inflammatory, cytotoxic and antioxidant potentials

Abstract

Hypericum L. is a significantly important genus for flora of Türkiye due to its richness in phytochemicals possessing medicinal and cosmetic benefits. The essential oil composition and biological activities of Hypericum bilgehan-bilgilii Basköse & Savran (HEO) were analysed for the first time in the present study. The volatile oil of the whole parts of H. bilgehan-bilgilii was obtained by hydrodistillation with Clevenger-type apparatus. The chemical composition was analyzed by GC-MS using non-polar column. α-glucosidase and 5-lipoxygenase inhibitory, cytotoxic, and DPPH radical scavenging activities were investigated. Forty-eight components were identified and represented 97.3% of the whole constituents. Interestingly, the major volatiles: 1-(2,4,5-trimethoxyphenyl)butan-1-one (27.7%) and 3-Methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one (11.2%) were detected for the first time in Hypericum essential oils. The oil exhibited a significant activity against the 5-lipoxygenase enzyme with an IC50 value of 39 µg/mL. Cytotoxicity potential of HEO was investigated, at different concentrations, towards four cell lines and, IC50 values underlies mild cytotoxicity. These results indicated that H. bilgehan-bilgilii essential oil may be considered as a valuable source for bioactive ingredients and anti-inflammatory agents.

Keywords

• Aromatherapy
• Gas Chromatography-Mass Spectrometry
• GC-FID
• Bioactivity
• Hypericum bilgehan-bilgilii

Ethical Statement

Since the article does not contain any studies with human or animal subject, its approval to the ethics committee was not required.

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