SALT TOLERANCE AND PHB PRODUCTION OF Rhizobium FRANK ISOLATES OF ROOT NODULES OF WILD Vicia aintabensis BOISS. & HAUSSKN. EX BOISS

Abstract

This study was performed in order to determine the tolerance of Rhizobium Frank isolates obtained from root nodules of wild Vicia aintabensis Boiss. & Hausskn. ex Boiss to different NaCl concentrations (0, 100, 150, 200, 250, 300, 350, 400 and 450mM) and to determine the effects of these different salt concentrations on poly-β-hydroxybutyric acid (PHB) production of the isolates. The results showed that the isolates showed different salt tolerance responses. V4.6 isolate was found to be the most affected isolate by salt concentrations. The PHB yield of the isolates (in terms of cell dry weight) was between 0,62-27%. Increasing salt concentrations inhibited isolate growths but stimulated PHB accumulation. PHB production by root nodule isolates under salt–stress conditions was discussed. 

Keywords

• Poly-β-hydroxybutyric acid,wild Vicia aintabensis Boiss. & Hausskn. ex Boiss,isolate,salt concentration

YABANİ Vicia aintabensis BOISS. & HAUSSKN. EX BOISS KÖK NODÜLLERİNDEN IZOLE EDİLEN Rhizobium FRANK İZOLATLARININ TUZ TOLERANSI VE PHB ÜRETİMİ

Öz

Bu çalışmada, yabani Vicia aintabensis Boiss. & Hausskn. ex Boiss kök nodüllerinden izole edilen Rhizobium Frank izolatlarının farklı tuz konsantrasyonlarına (0, 100, 150, 200, 250, 300, 350, 400 ve 450mM) toleranslarının ve bu tuz konsantrasyonlarının izolatların poli-β-hidroksibütirik asit (PHB) üretimlerine olan etkilerinin belirlenmesi amaçlanmıştır. Elde edilen sonuçlar, izolatların farklı tuz toleransına sahip olduklarını ortaya koymuştur. Tuz konsantrasyonlarından en fazla etkilenen V4.6 izolatı olmuştur. İzolatların PHB verimi (kuru hücre ağırlığına göre) %0,62-27 arasındadır. Artan tuz konsantrasyonları izolat gelişimini inhibe ederken, PHB depolanmasını teşvik etmiştir. Kök nodül izolatları tarafından tuz stres koşullarında PHB üretimi tartışılmıştır.

Anahtar Kelimeler

• Poli-β-hidroksibütirik asit,yabani Vicia aintabensis Boiss. & Hausskn. ex Boiss,izolat,tuz konsantrasyonu

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