FcMgv1, FcStuA AND FcVeA BASED GENETIC CHARACTERIZATION IN Fusarium culmorum (W.G. Smith)

Öz

Dünya çapında pek çok ülkede olduğu gibi Türkiye’de de Fusarium culmorum (W.G. Smith) başak yanıklığı ve kök çürüklüğü hastalıkları ile arpa ve buğday tarım alanlarında ekonomik kayıplara yol açar. Bu çalışmada, Türkiye’den köken alan 33 F. culmorum izolatının in vitro büyüme kapasitesi ile FcMgv1, FcStuA ve FcVeA genetik benzerliği aracılığı ile elde edilen fenotipik ve genetik karakterlerin ilişkisi incelenmiştir. Doğrusal büyüme oranı değerleri inkübasyonun 4. ve 7. günlerinde kaydedilmiştir. Ortalama doğrusal büyüme oranlarının 7,58±1.06 ve 14,7±1.26mm/gün arasında olduğu görülmüştür. Göreceli olarak yüksek LGR değerlerine sahip olduğu belirlenen F2 ile 18F izolatları ile göreceli olarak düşük LGR değerlerine sahip olduğu belirlenen 12F ve F19 izolatları multilokus temelli genotiplendirme analizlerinde kullanılmak üzere seçilmiştir. FcMgv1, FcStuA ve FcVeA genlerine ait sırasıyla 1733, 2001 ve 1898bç ürünler elde edilmiştir. Genler dizilenmiş, bir araya getirilmiş ve BLASTn ile maksimum olasılık topoloji analizi yapılmıştır. Her bir genin nükleotid dizisi NCBI'da 0,0-0,0 E-değeri ve 1188-3256 arası bit skoru vermiştir. Hizalama analizi en az %89 ön yükleme değeri ile sonuçlanmıştır. Ayrıca, benzer büyüme oranına sahip izolatlar filogenetik analizlerde aynı alt kümede yer almıştır. Bu çalışmada elde edilen bulgular, fungal yaşam için gerekli olan bu üç genin, genetik karakterizasyonda ve fenotipik ve genotipik özellikleri arasında ilişki kurulmasında kullanılabileceğini ortaya konmuştur. 

Anahtar Kelimeler

• Fusarium culmorum,PCR,genetic characterization,genetic diversity,linear growth rate

FcMgv1, FcStuA AND FcVeA BASED GENETIC CHARACTERIZATION IN Fusarium culmorum (W.G. Smith)

Abstract

Fusarium culmorum (W.G. Smith) leads to economic losses in wheat and barley fields in Turkey as well as in many countries worldwide as a result of head blight and crown rot diseases. In this study, in vitro growth capacity of 33 F. culmorum isolates originating from Turkey and the relationship between phenotypic and genetic characteristics obtained based on similarities of FcMgv1, FcStuA and FcVeA genes were investigated. Linear growth rate values were recorded at 4^th and 7^th days of incubation. The mean linear growth rate values ranged from 7.58±1.06 to 14.7±1.26mm/day. The isolates F2 and 18F with relatively high linear growth values and the isolates 12F and F19 with relatively low linear growth values, were selected to be used in multiloci based genotyping analysis. FcMgv1, FcStuA and FcVeA genes were amplified in lengths of 1733, 2001 and 1898bp, respectively. The genes were sequenced, aligned and then subjected to BLASTn and to maximum likelihood topology analysis. Nucleotide sequence of each gene showed maximum hit with associated genes deposited in NCBI with 0.0-0.0 E-values and 1188 to 3256 bit scores. Alignment analysis resulted in at least 89% bootstrap support. Moreover, isolates with similar linear growth rates were co-clustered in phylogenetic analysis. The findings obtained in this study showed that the three genes which are essential for fungal survival could be used in genetic characterization analysis and in revealing the associations between their genetic and phenotypic characteristics.

Keywords

• Fusarium culmorum,PCR,genetic characterization,genetic diversity,linear growth rate

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