Poecilimon zonatus Bolívar (ORTHOPTERA, TETTIGONIIDAE) REVISITED: GENETIC DATA REVEALED TWO NEW SPECIES AND ONE NEW SUBSPECIES

Abstract

Poecilimon Fischer (Orthoptera, Tettigoniidae) is the most species-rich genus of the respective order and family in Anatolia. However, intrageneric diversity still needs to be documented. Poecilimon zonatus Bolívar is a species with a relatively wide range of distribution in Anatolia compared to other congeners. The present study aims to examine the diversity of P. zonatus using an integrated approach. The diversity of the species was documented using data based on DNA sequences, male calling song and morphology. Automatic species delimitation tests applied to genetic data revealed a cryptic diversity in P. zonatus complex and suggested two new species and one new subspecies. The song data supported the new species, but morphology remained less productive in this respect. The new taxa defined in the light of the obtained data are; Poecilimon isozonatus sp. n., Poecilimon ciplaki sp. n. as Poecilimon ciplaki ciplaki subsp. n. and Poecilimon ciplaki denizliensis subsp. n. These taxa together with P. zonatus were considered a species complex within P. zonatus group. The phylogenetic tree produced from nad2 gene sequences supported the following relationships of P. tauricola + (P. ciplaki + (P. zonatus +P. isozonatus)) relationship.

Keywords

• Anatolia,Poecilimon isozonatus sp. n.,Poecilimon ciplaki sp. n.,Poecilimon ciplaki ciplaki subsp. n.,Poecilimon ciplaki denizliensis subsp. n.,phylogeny

Poecilimon zonatus Bolívar (ORTHOPTERA, TETTIGONIIDAE) REVISITED: GENETIC DATA REVEALED TWO NEW SPECIES AND ONE NEW SUBSPECIES

Öz

Poecilimon Fischer (Orthoptera, Tettigoniidae), dahil olduğu takım ve familyanın Anadolu’da tür sayısı en fazla olan cinstir. Ancak cins içi çeşitlilik halen ortaya konmayı beklemektedir. Cinsin diğer türleri ile karşılaştırıldığında Poecilimon zonatus Bolívar Anadolu’da nispeten geniş yayılışa sahip bir türdür. Bu çalışma entegre bir yaklaşımla türün çeşitliliğini saptamayı amaçlamaktadır. DNA dizileri, erkek çağrı sesi ve morfolojiden üretilen verilerle P. zonatus türünün çeşitliliği tanımlanmıştır. Genetik verilere uygulanan otomatik tür sınırları testleri P. zonatus’un kriptik bir çeşitlilik içerdiğini ve iki yeni tür ile bir yeni alttürün varlığına işaret etmiştir. Erkek çağrı sesi verileri yeni türleri desteklemiştir. Ancak, bu açıdan morfoloji daha az kullanışlı olmuştur. Elde edilen bu veriler ışığında tanımlanan yeni taksonlar şunlardır; Poecilimon isozonatus sp. n. Poecilimon ciplaki sp. n. ve Poecilimon ciplaki ciplaki subsp. n. ve Poecilimon ciplaki denizliensis subsp. n. Bu yeni taksonlar P. zonatus ile birlikte P. zonatus kompleksi olarak tanımlanmış ve bu kompleksin P. zonatus tür grubu içinde yer aldığı sonucuna varılmıştır. nad2 gen dizilerinden üretilen filogenetik ağaç P. tauricola + (P. ciplaki + (P. zonatus +P. isozonatus)) akrabalık ilişkisini desteklemiştir.

Anahtar Kelimeler

• Anatolia,Poecilimon isozonatus sp. n.,Poecilimon ciplaki sp. n.,Poecilimon ciplaki ciplaki subsp. n.,Poecilimon ciplaki denizliensis subsp. n.,phylogeny.

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