IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L.

Nuriye Meraklı; İbrahim Bulduk; Abdul Memon

doi:10.23902/trkjnat.978842

Research Article

IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L.

Year 2022, , 15 - 27, 15.04.2022

Nuriye Meraklı İbrahim Bulduk , Abdul Memon

https://doi.org/10.23902/trkjnat.978842

Cited By: 2

Abstract

Copper (Cu) is one of the essential trace metals required for plant growth. High amount of Cu in the media inhibits plant growth and is toxic to the plants. Brassica nigra L., a Cu accumulator, can tolerate a high amount of Cu and have specific mechanisms to relocate Cu within the cell compartments and keep the toxic amount of Cu away from the cytoplasm. This study aimed to evaluate the Cu-induced gene expression pattern of B. nigra Diyarbakir ecotype subjected to low Cu treatment. The Arabidopsis ATH1 genome array was used to determine the Cu-induced gene expression in the leaves of B. nigra grown at 25 µM Cu. Ninety-five genes were upregulated, and seventy-two genes were downregulated in the leaves of plants grown under 25 µM Cu. Cu responsive genes, such as glutathione S-transferase, glutathione reductase, heavy metal transporters, natural resistance-associated macrophage proteins, cytochrome p450, MYB-like transcription factor, copper/zinc, and Fe superoxide dismutases, and some protein kinases were highly expressed in the leaves of Cu-treated plants. The present work provides the global gene expression pattern in facultative metallophyte B. nigra, which could serve as a molecular tool for future phytoremediation studies.

Keywords

copper, microarray, Cu-induced genes, B. nigra Diyarbakir ecotype

Supporting Institution

Uşak University

Project Number

BAP-MF005

Thanks

We thank Ahmet AKBUGA (Manisa, Turkey), President of the Chamber of Agriculture Akhisar, and their technicians for their assistance in metal analysis.

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Year 2022, , 15 - 27, 15.04.2022

Nuriye Meraklı İbrahim Bulduk , Abdul Memon

https://doi.org/10.23902/trkjnat.978842

Cited By: 2

Abstract

Bakır (Cu), bitki büyümesi için gerekli olan temel eser metallerden biridir. Ancak, ortamdaki yüksek miktarda Cu, bitkide toksik etki göstererek büyümeyi olumsuz yönde etkilemektedir. Diğer bir yandan, Cu akümülatörü olan Brassica nigra L., sahip olduğu özel mekanizmalar ile yüksek Cu miktarlarını akümüle edebilmekte ve bu metali farklı hücre bölümlerine taşıyarak sitoplazmadan uzaklaştırabilmektedir. Bu çalışmada, B. nigra’nın düşük Cu seviyesindeki gen anlatım profilinin tespiti amaçlanmıştır. Düşük Cu seviyesinde yetiştirilen B. nigra'nın yapraktaki Cu ile indüklenen gen anlatım profili Arabidopsis ATH1 genom çipi kullanılarak tespit edilmiştir. Elde edilen sonuçlara göre, B. nigra’da 95 gen yukarı regüle ve 72 gen aşağı regüle olarak tanımlanmıştır. Glutatyon S-transferaz, glutatyon redüktaz, ağır metal taşıyıcılar, doğal dirençle ilişkili makrofaj proteinleri, sitokrom p450, MYB-gibi transkripsiyon faktörü, bakır/çinko ve Fe süperoksit dismutazlar gibi Cu ile ilişkili bazı genlerin ve bazı protein kinazların yüksek oranda anlatımının olduğu saptanmıştır. Bu çalışma, fakültatif metalofit olarak tespit edilen B. nigra' nın global gen anlatım profilini sunmakta olup, moleküler bir araç olarak ileriki fitoremediasyon çalışmaları için yardımcı olacaktır.

Project Number

BAP-MF005

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36. Memon, A.R. & Zahirovic, E. 2014. Genomics and Transcriptomics Analysis of Cu Accumulator Plant Brassica nigra L. Journal of Applied Biological Sciences, 8: 01-08.
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Details

Primary Language	English
Subjects	Structural Biology
Journal Section	Research Article/Araştırma Makalesi
Authors	Nuriye Meraklı This is me 0000-0001-5454-7149 İbrahim Bulduk 0000-0001-6172-7738 Abdul Memon 0000-0001-9447-6453
Project Number	BAP-MF005
Publication Date	April 15, 2022
Submission Date	August 4, 2021
Acceptance Date	October 16, 2021
Published in Issue	Year 2022

Cite

APA	Meraklı, N., Bulduk, İ., & Memon, A. (2022). IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L. Trakya University Journal of Natural Sciences, 23(1), 15-27. https://doi.org/10.23902/trkjnat.978842
AMA	Meraklı N, Bulduk İ, Memon A. IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L. Trakya Univ J Nat Sci. April 2022;23(1):15-27. doi:10.23902/trkjnat.978842
Chicago	Meraklı, Nuriye, İbrahim Bulduk, and Abdul Memon. “IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica Nigra L”. Trakya University Journal of Natural Sciences 23, no. 1 (April 2022): 15-27. https://doi.org/10.23902/trkjnat.978842.
EndNote	Meraklı N, Bulduk İ, Memon A (April 1, 2022) IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L. Trakya University Journal of Natural Sciences 23 1 15–27.
IEEE	N. Meraklı, İ. Bulduk, and A. Memon, “IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L”., Trakya Univ J Nat Sci, vol. 23, no. 1, pp. 15–27, 2022, doi: 10.23902/trkjnat.978842.
ISNAD	Meraklı, Nuriye et al. “IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica Nigra L”. Trakya University Journal of Natural Sciences 23/1 (April 2022), 15-27. https://doi.org/10.23902/trkjnat.978842.
JAMA	Meraklı N, Bulduk İ, Memon A. IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L. Trakya Univ J Nat Sci. 2022;23:15–27.
MLA	Meraklı, Nuriye et al. “IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica Nigra L”. Trakya University Journal of Natural Sciences, vol. 23, no. 1, 2022, pp. 15-27, doi:10.23902/trkjnat.978842.
Vancouver	Meraklı N, Bulduk İ, Memon A. IDENTIFICATION OF GENES REGULATED IN RESPONSE TO Cu EXPOSURE IN Brassica nigra L. Trakya Univ J Nat Sci. 2022;23(1):15-27.

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