INVESTIGATION OF THE GENETIC STRUCTURE OF SOME ANATOLIAN Achillea L. (ANTHEMIDEAE, ASTERACEAE) POPULATIONS USING THE ISSR MARKERS

Yıl 2018, Cilt: 19 Sayı: 1, 1 - 10, 15.04.2018

Efkan Bağda

https://doi.org/10.23902/trkjnat.346537

Öz

Due to high level of hybridization and polyploidy, the
perennial and allogamic Achillea L. genus
with a complex phyletic structure has about 142 species widely distributed over
in the Northern Hemisphere. The genus is widely distributed in Turkey with 48
species half of which are endemic. The gene and diversification center of the Santolinoideae section including 18
endemic species is thought to be in Anatolia. There exist no comprehensive
molecular phylogenetic study on Achillea
whose morphological revision in Turkey was completed in 2006.

In the study, phylogenetic analyzes
were performed using 10 different oligonucleotides for amplification of ISSR
bands based on 74 samples of 18 species from 3 sections of Achillea genus. All the oligonucleotides analyzed were found to be
polymorphic. The total number of loci is 135 and 44 (33%)
of them are parsimony informative. Serious topological differences
showing that Achillea taxa include
both monophyletic and polyphyletic lineages were revealed in phylogenetic trees
obtained under UPGMA, MP and BI methods. Sections were not clearly separated in
trees with clear species separations. The results of UPGMA and MP analyses
showed that A. vermicularis Trin. was
placed as the outgroup while A.
sipirokorensis Hausskn. & Bornm. and A. sintenisii Hub.-Mor. formed the outgroup together in Bayesian
Inference analysis (BI). The obtained clusters of PCA based on binary genetic
distance values were consistent with the result of BI analysis. Molecular
variation analysis showed that almost all of the molecular variation was
completely resulted from variations within populations.

Anahtar Kelimeler

Achillea, ISSR, molecular phylogeny, Turkey

Kaynakça

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