Süt Sığırlarında Ketozis Direnci için Gen Zenginleştirme ve Yolak Analizi: GWAS Tabanlı Bir Yaklaşım

Year 2024, , 1014 - 1022, 12.10.2024

Veysel Bay

https://doi.org/10.30910/turkjans.1522944

Abstract

Süt sığırlarında ketozis, geç gebelik döneminden erken laktasyon dönemine geçişte ortaya çıkan yaygın bir metabolik bozukluktur. Bu durum, enerji alımı ile harcaması arasındaki dengesizlikten kaynaklanır ve keton cisimciklerinin aşırı birikimine yol açar. Ketozis, sığır sağlığını ve verimliliğini önemli ölçüde etkileyebilir. Genom araştırmalarındaki, özellikle de genom boyu dizileme analizleri (GWAS) gibi son gelişmeler, ketozis direncine katkıda bulunan genetik faktörleri keşfetme fırsatı sunmaktadır.
Bu araştırmanın amacı, süt sığırlarında ketozis direnci ile ilişkili potansiyel fonksiyonel aday gen yolaklarını belirlemek için var olan GWAS verilerini gen zenginleşmesi analizi kullanarak kapsamlı bir şekilde incelemek ve analiz etmektir. Bu çalışmada yedi farklı çalışmadan elde edilen verileri incelenmiş ve filtreleme sonrası 640 tekrarsız gen elde edilmiştir. Tanımlanan genlerin insan homologları ile Enrichr adlı çevrimiçi gen anotasyon aracı kullanılarak yolak analizi yapılmıştır. Bulgularımız, trigliserit metabolizmasının düzenlenmesini ve ketozis sırasında metabolik dengenin korunmasında şilomikronların rolünü ve asilgliserol homeostaz yolaklarını ön plana çıkarmaktadır. Ayrıca, immün yanıt yolaklarının ketozis ile ilişkili genlerle bağlantılı olduğu bulunmuştur, bu da metabolik yolaklar ve immün yolaklar arasındaki karmaşık etkileşimlere dair bilgiler sunmaktadır.
Bu çalışma, süt sığırlarında metabolik sağlığı ve verimliliği arttırmayı amaçlayan yetiştirme stratejilerinin geliştirilmesinde genetik faktörlerin anlaşılmasının önemini vurgulamaktadır. Gelecek araştırmalar, bu aday genlerin doğrulanmasına ve mekanistik rollerinin incelenmesine odaklanarak, hedefe yönelik müdahaleleri kolaylaştırmalı ve süt sürülerinde ketozis direncini artırmalıdır.

Keywords

Süt sığırlarında ketosis, GWAS, Gen zenginleştirme

Gene Enrichment and Pathway Analysis for Ketosis Resistance in Dairy Cattle: A GWAS-Based Approach

Abstract

Ketosis in dairy cattle is a common metabolic disorder that arises during the transition period from late gestation to early lactation. It is primarily caused by an imbalance between energy intake and expenditure, leading to an excessive accumulation of ketone bodies. This condition can significantly affect cattle health and productivity. Recent advances in genomic research, especially genome-wide association studies (GWAS), offer an opportunity to explore the genetic factors that contribute to ketosis resistance.
The aim of this study is to comprehensively review and analyze existing GWAS data using gene enrichment analysis to identify potential functional candidate gene pathways associated with ketosis resistance in dairy cattle. In this study, data obtained from seven different studies were examined and 640 non-repetitive genes were obtained after filtering. Using Enrichr, an online tool for gene annotation, pathway analysis was performed with human homologs of the identified genes. Our findings highlight the acylglycerol homeostasis pathway, the regulation of triglyceride metabolism, and the role of chylomicrons in maintaining metabolic balance during ketosis. Additionally, immune response pathways were found to be linked to the genes associated with ketosis, offering insights into the intricate interplay between metabolic and immune pathways in ketosis.
This study emphasizes the importance of understanding genetic factors in developing breeding strategies aimed at enhancing metabolic health and productivity in dairy cattle. Future research should focus on validating these candidate genes and exploring their mechanistic roles to facilitate targeted interventions and improve resistance to ketosis in dairy herds.

Keywords

Dairy cattle ketosis, GWAS, gene enrichment

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