Abstract
Gül sarı damar virüsü güllerin son yıllarda tanılanmış bir virüs hastalığı olup dünyada son derece sınırlı bir alanda varlığı bilinmektedir. Ancak ülkemizde gerçekleştirilen çalışmalarda etmenin varlığı farklı bölge ve illerde tespit edilmiştir. Virüs hastalıklarının diğer biyotik hastalıklarda olduğu gibi doğrudan kimyasal mücadelesi mümkün olmadığı için etmenin moleküler karakterizasyonu gelecek yıllarda yapılması muhtemel dayanıklılık ya da gen susturma çalışmaları için temel oluşturmaktadır. Böylece ülkemizde daha önceden tespit edilen 44 RYVV izolatı tüm genom çalışmaları için kullanılmıştır. Tüm genom çalışmalarında türe özgü 7 farklı primer çifti ile overlapping PCR yöntemi uygulanmıştır. Bu izolatların arasından 3 tanesinin tüm genomu elde edilmiştir. Diğer izolatların ise tüm genomu elde edilememiştir. Tüm genomu elde edilen 3 RYVV izolatının 9314 nükleotit büyüklüğünde olduğu belirlenmiştir. Genbankasında bulunan tek RYVV izolatı ile Türkiye izolatlarının da birbirleri ile yüksek oranda nükleotit dizisi benzerliği gösterdiği belirlenmiştir. Bununla birlikte, diğer izolatların tüm genomunun elde edilememesi ve elde edilenlerin de GenBankası veri tabanındaki bulunan tek izolata göre tasarlanmış olan primer çiftleri ile amplifiye edilmesinden dolayı RYVV izolatlarının farklı strainlerinin bulunmasından kaynaklanabilir. Bu sebeple gelecek çalışmalarda tam genom bilgilerinin çıkarılması için yeni nesil sekanslama çalışmalarına öncelik verilmesi gerektiği düşünülmektedir.
Keywords
Ethical Statement
This study was supported by a grant from Çanakkale Onsekiz Mart University
Project Number
FBA-2019-2891
References
- Mollov, D., Lockhart, B., Zlesak, D., 2009. Genome characterization and transmission of rose yellow vein virus, a new caulimovirus occurring in garden rose. Phytopathology, 99, 87.
- Mollov, D., Lockhart, B., Zlesak, D., 2013a. Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family potyviridae. Arch. Virol. 158, 1917-1923.
- Karanfil, A. 2021. Prevalence and molecular characterization of Turkish isolates of the rose viruses. Crop Protection, 143, 105565.
- Karanfil, A., Randa-Zelyüt, F., Ertunç, F., Korkmaz, S., 2018. First report of rose yellow vein virus in Turkey. New Dis. Rep. 38, 11.
- Perez-Egusquiza, Z., Liefting, L.W., Ward, L.I., 2013. First report of rose yellow vein virus in Rosa sp. in New Zealand. Plant Dis. 97, 1122-1122.
- Culal-Kiliç, H., Yardimci, N., Gubur, S., 2017. Serological, biological and Molecular detection of prunus necrotic ringspot virus on Rosa damascena Mill. in Turkey. Acta Sci. Pol-Hortoru. 16, 145-150.
- Sertkaya, G. 2010. An investigation on rose mosaic disease of rose in Hatay-Turkey. Julius-Kühn-Archiv, 427, 309-313.
- Sipahioğlu, H.M., Çağlar, B.K., Baloğlu, S., 2001. Determination of the incidence of PNRSV and ApMV rose viruses in rose by ELISA in the Eastern Mediterranean region. IX Congress of Phytopathology, 3–8 September, Tekirdağ-Turkey, 572–577.
- Muhire, B.M., Varsani, A., Martin, D.P., 2014. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PloS One, 9, e108277.
- Çevik, B., Yardımcı, N., Culal-Kılıç, H., 2011. Detection of viruses infecting stone fruits in Western Mediterranean region of Turkey. Plant Pathol. J. 27, 44-52.
- Jiang B., Hong N., Wang G.P., Hu J., Zhang J.K., Wang CX., Liu Y., Fan X.D., 2008. Characterization of Citrus tristeza virus Strains from Southern China Based on Analysis of Restriction Patterns and Sequences of Their Coat Protein Genes. Virus Genes, 37 (2), 185-92.
- Li, R., Mock, R., Huang, Q., Abad, J., Hartung, J., Kinard, G., 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. J. Virol. Methods. 154, 48-55.
- Milleza, E.J.M., Ward, L.I., Delmiglio, C., Tang, J.Z., Veerakone, S., Perez-Egusquiza, Z., 2013. A survey of viruses infecting Rosa spp. in New Zealand. Australas. Plant Pathol. 42, 313-320.
- Lockhart, B., Zlesak, D., & Fetzer, J. (2008, April). Identification and partial characterization of six new viruses of cultivated roses in the USA. In XII International Symposium on Virus Diseases of Ornamental Plants 901 (pp. 139-147).
Abstract
Rose yellow vein virus (RYVV) is a viral agent that has been identified in roses in recent years and is known to exist in a very limited area in the world. However, in studies conducted in our country, the presence of the agent has been detected in different regions and provinces. Since direct chemical control of viral diseases is not possible as in other biotic diseases, molecular characterization of the agent forms the basis for possible resistance or gene silencing studies to be conducted in the coming years. Thus, 44 RYVV isolates previously identified in our country were used for full genome studies. In full genome studies, overlapping PCR method was used with 7 different species-specific primer pairs. The full genomes of 3 of these isolates were obtained. The full genomes of the other isolates could not be obtained. It was determined that the genome size of the 3 RYVV isolates whose full genomes were obtained was 9314 nucleotides. It was determined that the only RYVV isolate found in the GenBank and the Türkiye isolates also showed a high level of nucleotide sequence similarity. However, the fact that the entire genome of the other isolates could not be obtained and the ones obtained were amplified with primer pairs designed according to the single isolate in the GenBank database may be due to the presence of different strains of RYVV isolates. For this reason, it is thought that next-generation sequencing studies should be prioritized to obtain complete genome information in future studies.
Keywords
Ethical Statement
This study was supported by a grant from Çanakkale Onsekiz Mart University
Project Number
FBA-2019-2891
References
- Mollov, D., Lockhart, B., Zlesak, D., 2009. Genome characterization and transmission of rose yellow vein virus, a new caulimovirus occurring in garden rose. Phytopathology, 99, 87.
- Mollov, D., Lockhart, B., Zlesak, D., 2013a. Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family potyviridae. Arch. Virol. 158, 1917-1923.
- Karanfil, A. 2021. Prevalence and molecular characterization of Turkish isolates of the rose viruses. Crop Protection, 143, 105565.
- Karanfil, A., Randa-Zelyüt, F., Ertunç, F., Korkmaz, S., 2018. First report of rose yellow vein virus in Turkey. New Dis. Rep. 38, 11.
- Perez-Egusquiza, Z., Liefting, L.W., Ward, L.I., 2013. First report of rose yellow vein virus in Rosa sp. in New Zealand. Plant Dis. 97, 1122-1122.
- Culal-Kiliç, H., Yardimci, N., Gubur, S., 2017. Serological, biological and Molecular detection of prunus necrotic ringspot virus on Rosa damascena Mill. in Turkey. Acta Sci. Pol-Hortoru. 16, 145-150.
- Sertkaya, G. 2010. An investigation on rose mosaic disease of rose in Hatay-Turkey. Julius-Kühn-Archiv, 427, 309-313.
- Sipahioğlu, H.M., Çağlar, B.K., Baloğlu, S., 2001. Determination of the incidence of PNRSV and ApMV rose viruses in rose by ELISA in the Eastern Mediterranean region. IX Congress of Phytopathology, 3–8 September, Tekirdağ-Turkey, 572–577.
- Muhire, B.M., Varsani, A., Martin, D.P., 2014. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PloS One, 9, e108277.
- Çevik, B., Yardımcı, N., Culal-Kılıç, H., 2011. Detection of viruses infecting stone fruits in Western Mediterranean region of Turkey. Plant Pathol. J. 27, 44-52.
- Jiang B., Hong N., Wang G.P., Hu J., Zhang J.K., Wang CX., Liu Y., Fan X.D., 2008. Characterization of Citrus tristeza virus Strains from Southern China Based on Analysis of Restriction Patterns and Sequences of Their Coat Protein Genes. Virus Genes, 37 (2), 185-92.
- Li, R., Mock, R., Huang, Q., Abad, J., Hartung, J., Kinard, G., 2008. A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. J. Virol. Methods. 154, 48-55.
- Milleza, E.J.M., Ward, L.I., Delmiglio, C., Tang, J.Z., Veerakone, S., Perez-Egusquiza, Z., 2013. A survey of viruses infecting Rosa spp. in New Zealand. Australas. Plant Pathol. 42, 313-320.
- Lockhart, B., Zlesak, D., & Fetzer, J. (2008, April). Identification and partial characterization of six new viruses of cultivated roses in the USA. In XII International Symposium on Virus Diseases of Ornamental Plants 901 (pp. 139-147).
Details
| Primary Language | English |
|---|---|
| Subjects | Phytopathology |
| Journal Section | Research Article |
| Authors | |
| Project Number | FBA-2019-2891 |
| Early Pub Date | October 12, 2024 |
| Publication Date | October 12, 2024 |
| Submission Date | September 6, 2024 |
| Acceptance Date | September 26, 2024 |
| Published in Issue | Year 2024 |