Race and Virulence Dynamics of Puccinia triticina and Effectiveness of Lr genes in Bulgaria During 2005 – 2009

Vanya Ivanova

Race and Virulence Dynamics of Puccinia triticina and Effectiveness of Lr genes in Bulgaria During 2005 – 2009

Year 2014, Volume: 1 Issue: Özel Sayı-1, 709 - 720, 01.03.2014

Abstract

Durable and efficient resistance of wheat to Puccinia triticina can be ensured only through good knowledge on the structure of the pathogen population. During 2005-2009 the structure and variability in the population of brown rust was investigated at Dobroudja Agricultural Institute – Bulgaria. Eight standard races were identified, which was an indication that the great race variability of the pathogen can not be encompassed by using the standard differential set. Therefore the race variability was determined with the help of isogenic lines applying the nomenclature suggested at COST 817. During the investigated period, 172 phenotypically different pathotypes were identified, pathotypes 63562 (41%), 63573 (20%) and 63572 (19.4%) being predominant. The genetic variability within the population was represented through 236 gene formulae of virulence, 106 of which were detected for the first time in the population. Highly efficient were genes Lr1, Lr9, Lr15, Lr28 and Lr42. During the period of investigation, pathotypes overcoming the resistance of gene Lr19 were identified thus decreasing significantly its efficiency

Keywords

wheat; brown rust; pathotypes; virulence; genes

References

Dodov, D., 1934. Physiological races of black rust on wheat communications of Sofia University, 1933- 1934 (in Bg). Research
Karzhin H., M. Stefcheva, V. Kiryakova, 2003. Virulence of Puccinia recondita f . sp. tritici during 2000 - 2001. Plant breeding sciences, 40: 360-365 (in Bg).
Kiryakova, V., 2007. Virulence variability of P. recondita f. sp. tritici in Bulgaria during 2002 - 2004. International conference “Plant gene pool – the basis of modern agriculture“, 14 - 16.06. Institute of plant and genetic resources - Sadovo, 589-593 (in Bg).
Markelova, Т. С., 2007. Immunological bases and methods for developing of initial breeding material of wheat for breeding for resistance to diseases in the Volga region, Ph.D. thesis, Saratov (in Rus).
Stefcheva М., S. Maneva, 2006. Investigation on the virulence of Puccinia recondita f. sp. tritici in Bulgaria. Plant breeding sciences, 4: 340- 343 (in Bg).
Todorova М. 1999. Race and genetic analysis on the population of Puccinia recondita f. sp. tritici in Bulgaria during 1996 – 1997. Plant breeding sciences, 36 (2): 45-47 (in Bg).
Todorova М., V. Kiryakova, 2000. Physiological specialization of Puccinia recondita f. sp. tritici in Bulgaria in 1998. Proceedings, 75 years of agroforestry education in Bulgaria, Sofia., 127-132 (in Bg).
Bartoš P., Stuchlikova E., Hanusova R. 1996. Adaptation of wheat rust to the wheat cultivars Euphytica, Vol. 92: 95-103. Czechoslovakia ,
Bartoš P., Huszar, J. 1998. Virulence of the wheat leaf rust population in Slovakia in 1996, Biologia,Vol. 53(1): 99-105.
Bartoš P., Huszar, J., Herzova, E.1999. Virulence of Wheat Leaf Rust in Slovakia 1997-1998. Plant Protection Science, Vol 35 (3): 85 - 92.
Bartoš P., Huszar, J, Hanzalova, A., Herzova, E. 2001. Wheat leaf rust races / pathotypes in Slovakia in 1999-2000. Plant Protection Science, Vol 37 (3): 85 - 90.
Bhardwaj S. C., M. Prashar, S. Kumar, S. K. Jain, and D. Datta 2005. Lr 19 resistance becomes susceptible to Puccinia triticina in India. Plant Disease, 89: 1360.
Green 1965. Stem rust of wheat, rye and barley in Canada in 1964. Canadian Plant Disease, 45: 23 - 29.
Goyean H., R. Park, B. Schaeffer, Ch. Lannou. 2006. Distribution of pathotypes with regard to host cultivars in French wheat leaf rust populations. Phytopathology, 96: 264-273.
Hanzalova A. & P. Bartoš 2006. Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Czech Republic in 2001 - 2004. Czech J. Genet. Plant Breed., 42 (4): 126-131.
Hanzalova A., J. Huszar, P. Bartoš, E. Herzova, 2008. Occurrence of wheat leaf rust (Puccinia triticina) races and virulence changes in Slovakia in 1994 - 2004. Biologia 63 (2): 1 - 4.
Hanzalova A., 2010. Physiologic specialization of wheat leaf rust (Puccinia triticina Eriks.) in the Czech Republic in 2005-2008. Cereal Research Communications, 38 (3): 366 -374.
Huerta - Espino J. & R. Singh, 1994. First report of virulence to wheat with leaf rust resistance gene Lr19 in Mexico Plant Disease 78: 640.
Johnston, C & L. Browder 1966. Seventh revision of the International register of physiologic races of Puccinia recondita f. sp. tritici. Plant Diseases Reporter, 50: 756-760.
Kolmer J.A., Y. Jin & D.Long 2007. Wheat leaf and stem rust in the United States. Australian Journal of Agricultural Research, 58: 631- 638.
Lind V. & E. Gultyaeva 2007. Virulence Frequencies of Puccinia triticina in Germany and the European regions of the Russian Federation, J. Phytopathology 155: 13-21.
Limpert E. and Muller K. 1994. Designation of Pathotypes Phtopathology 140: 346-358. Pathogens, J.
Masar S., Bernard V., K. Masarova.2004. Virulence of Puccinia recondita f. sp. tritici in Slovakia in 2002. Acta fitotechnica et zootechnica, Proceedins of the XVI Slovak and Czech Plant Protection Conference, Nitra, Slovakia, Vol.7:196-197.
Masterhazy A., Bartoš P., Andersen O., Casulli F., Csosz M., Goyean H., Ittu M., Jones E., Manisterski J., Manninger K., Pasquini M., Rubiales D., Schachermayer G., Strembicka A., Todorova M., Unger O., Vida G.Y., Walter U., 2000. European virulence survey for leaf rust in wheat. Agronomie 20: 793-804.
Pasquini M., D. Pancaldi, F. Casulli 2003. Genetic variation in Italian populations of Puccinia recondita f. sp. tritici from 1990 to 2001. J. Genet & Breed. 57: 191-200.
Sibikeev, S. N., V. A. Krupnov, S. A. Voronina and V.A. Elesin 1996. First report of leaf rust pathotypes virulent to highly effective Lr – genes transferred from Agropyron species to bread wheat, Plant Breeding 115: 276-278.
Stakman, E.C., D.M. Stewart and W.Q. Loegering, 1962. Identification of physiological races of Puccinia graminis var. tritici. Agric. Res. Serv. E617 Agriculture: Washington DC). Department of
Todorova M. and V. Kiryakova 2001. Physiologic on specialization f.sp.tritici in Bulgaria in 1999. Cereal rust and [www.crpmb.org] recondita Powdery Mildews Bulletin
Winzeler M., Mesterhazy A., Park R. F., P. Bartoš, M. Csosz, H. Goyean, M. Ittu, E. Jones, F. Loschenberger, K. Manninger, M. Pasquini, K. Richter, D. Rubiales, G. Schachermayer, A. Strzembicka, M. Trottet, O. Unger, G. Vida, U. Walther 2000. Resistance of European winter wheat germplasm to leaf rust. Agronomie 20: 783-792.