Identification of I-gene for BCMV Resistance with Scar Marker SW13 in Phaseolus vulgaris L. Generations, Derived from the Cross (BAT 477 X Dobroudjanski Ran)

Year 2014, Volume: 1 Issue: Özel Sayı-1, 754 - 756, 01.03.2014

Vladimir Krastev Gancho Passev Dimitrina Kostovaprotochrıstova - -

Abstract

Resistance to BCMV (Bean common mosaic virus) was established in Phaseolus vulgaris variety BAT 477, following artificial inoculation with the virus. Crosses were performed between BAT 477 and BCMV susceptible parent Dobrujanski ran, a widely spread Bulgarian variety, in order to transfer the resistance in the generations. By PCR, using SCAR marker SW 13, the presence of I-gene for BCMV resistance was proved in donor parent BAT 477, in all F1 plants and in segregating F2. The monogenic control of resistance was demonstrated by 690 bp fragment amplification in 75 plants and absent of fragment in the remaining 31 plants. The ratio of segregation was approximately 3:1, as evidenced by the criterion “χ2" at level of significance 0,50>P>0,20

Keywords

Bean common mosaic virus (BCMV), I-gene, Phaseolus vulgaris L

Identification of I-gene for BCMV Resistance with Scar Marker SW13 in Phaseolus vulgaris L. Generations, Derived from the Cross (BAT 477 X Dobroudjanski Ran)

Abstract

Keywords

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References

• Drifhout, E., 1978. Genetic interaction between Phaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding for resistance. Agric Res Rep 872: 98 pp.
• Edwards, K., Johnstone, C., and Thompson, C. 1991. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. 19(6):1349. Acids Research
• Haley, S.D., Afanador, L., and Kelly, J.D. 1994. ldentification and application of a random amplified polymorphic DNA marker for the I gene (potyvirus resistance) Phytopathology, 84: 157-1 60. common bean.
• Kelly, J.D., Afanador, L., and Haley, S.D. 1995. Pyramiding genes for resistance to bean common mosaic virus. Euphytica, 82: 207-2 12.
• Khan, J. A., Lohuis, D., Goldbach, R., Dijkstra, J., 1993. Sequence data to settle the taxonomic position of bean common mosaic virus and blackeye cowpea mosaic virus isolates. Journal of General Virology 74:2243–2249.
• Mc Kern, N.M., Mink G.I., Barnett O.W., Mishra A., Whittaker L.A., Silgernagel M.J., Ward C. W., Shukla D. D., 1992. Isolates of Bean Common Mosaic Virus Comprising two distinct 82:923–929. Phytopathology
• Melotto, M., Afanador, L., Kelly, J. D., 1996. Development of a SCAR marker linked to the I gene in common bean. Genome 39(6):1216–1219.
• Sharma, P. N., Pathania, A., Kapil, R., Sharma, P., Sharma, O. P., Patial, M., & Kapoor, V. (2008). Resistance to bean common mosaic potyvirus strains and its inheritance in some Indian land races of common bean. Euphytica, 164(1), 173–180.
• Singh. S.P., Gepts, P., and Debouck, D.J. 1991. Races of common bean (Phaseolus vulgaris, Fabaceae). Econ. Bot. 45: 379-396.
• Vetten, H. J., Lesemann, D. E., Maiss, E., 1992. Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. Archives of virology Supplementum. 5:415– 431.