Profile of antioxidant enzymes in two Bulgarian barley cultivars at early growth stage, differing in salt stress response

Year 2014, Volume: 1 Issue: Özel Sayı-1, 811 - 816, 01.03.2014

Lyuben Zagorchev Plamena Kamenova Borislav Assenov Nabil Abumhadı Elena Todorovska Mariela Odjakova

Abstract

Soil salinization is among the major crop yield limiting factors in contemporary agriculture. Excessive irrigation and climate changes are among the causes for increased salinity in previously unaffected areas, including the Balkan Peninsula. The development of salt tolerant cultivars of existing crops is essential for meeting the growing food production necessity and for utilization of salinized agricultural areas. Marker assisted selection is among the promising approaches for fast and efficient screening of newly developed cultivars for tolerance to various stresses. The identification of genetic and protein markers for stress tolerance is an essential tool for accelerating of breeding programs and development of modern agriculture. Along with osmotic stress and ion toxicity, high NaCl concentrations negatively affect plant growth and development by promoting burst in reactive oxygen species formation. Antioxidative systems are essential for overcoming of this negative effect. In the present study two Bulgarian barley cultivars – Bozhin and IZ Bori were studied. Four days old seedlings were evaluated for their ability to grow at 0.15 and 0.3 M NaCl and proteins were isolated from roots and leaves. Antioxidant enzymes: peroxidases, ascorbate peroxidases, catalases, superoxide dismutases and glutathione reductases were studied. Enzymatic profiles obtained by zymographic analyses after electrophoretic separation showed several isoforms associated with salt stress response and salt stress tolerance. Further analyses and comparison of zymographic to genetic and metabolomic data would further reveal differences in the two cultivars and establish appropriate molecular markers for salt tolerance

Keywords

antioxidative enzymes, reactive oxygen species, salt stress, zymographic analysis

Profile of antioxidant enzymes in two Bulgarian barley cultivars at early growth stage, differing in salt stress response

Abstract

Keywords

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References

• Adem, G.D., Roy, S.J., Zhou, M., Bowman, J.P., Shabala, S. 2014. Evaluating contribution of ionic, components towards salinity tolerance in barley. BMC Plant Biol 14:113 stress
• Agricultural Academy, Institute of Agriculture Karnobat. 2014. Catalog of varieties of barley, oat and wheat, bred in the Institute of Agriculture - Karnobat
• Bhutta, W.M. 2011. Antioxidant activity of enzymatic system of two different wheat (Triticum aestivum L.) cultivars growing under salt stress. Plant Soil Environ 57: 101-107.
• Gill, S.S., Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 12: 909 – 930.
• Fan, Y., Zhu, M., Shabala, S., Li, C.D., Johnson, P., Zhou, M.X. 2014.Antioxidant activity in salt- stressed barley leaves: evaluating time- and age-dependence and suitability for the use as a biochemical marker in breeding programs. J Agro Crop Sci
• Food and Agriculture Organization of the United Nations. www.fao.org
• Kim, S.Y., Lim, J.H., Park, M.R., Kim, W.J., Park, T.I., Seo, Y.W., Choi, K.G., Yun, S.J. 2005. Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. J Biochem Mol Biol 38: 218-224.
• Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680 – 685.
• Maksimović, J.D., Zhang, J., Zeng, F., Živanović, B.D., Shabala, L., Zhou, M., Shabala, S. 2013. Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance? Plant Soil 365:141–155.
• Manchenko, G.P. 2003. Handbook of detection of enzymes on electrophoretic gels. CRC Press
• Munns, R., Tester, M. 2008. Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681.
• Munns, R. 2002. Comparative physiology of salt and water stress. Plant Cell Environ 25: 239-250. National Grain www.grain.bg Association. 2012.
• Noctor, G., Foyer, C.H. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Molec Biol 49:249–279.
• Yildiz, M., Terzi, H. 2013. Effect of NaCl stress on biosynthesis, chlorophyll peroxidation and antioxidative enzymes in leaves of salt-tolerant and salt-sensitive barley cultivars. J Agri Sci 19: 79-88. lipid