Effects of agar types on rooting performance in tissue culture: Sample of Quince A rootstock cultures

Year 2014, Volume: 1 Issue: Özel Sayı-1, 957 - 963, 01.03.2014

Melekber Sulusoglu

Abstract

s This study investigated the effects of the four commercial gelling agents from Merck, Bacto, Oxoid, and Gelrite on in vitro rooting of, one of the popular pear rootstocks, Quince A. IBA or NAA, at 0.5 gL−1concentration, was added in the rooting media. Different rooting rates were observed according to used agar and auxin types. The highest rooting rate was recorded in the medium containing 9 gL−1 Oxoid agar. Root number and length were best in Bacto agar, especially at its 7 gL−1 concentration. Gelrite was unsuitable for rooting of Quince A. Optimum concentrations for Merck (7 gL−1), Bacto (7 gL−1), and Oxoid (7 & 9 gL−1) agar were selected in the first experiment, then they were supplemented with 0.5 gL−1 IBA or 0.5 gL−1 NAA in the second experiment. IBA was more effective on root number and root length than NAA. Merck agar (7 gL−1) was the most economical, Bacto agar (7 gL−1) was the most effective on root quality of Quince A

Keywords

Agar types, Quince A, in vitro rooting, NAA, IBA, cost analysis

Effects of agar types on rooting performance in tissue culture: Sample of Quince A rootstock cultures

Abstract

Çalışmada 0.5 gL−1 IBA veya NAA eklenen dört farklı ticari agar tipinin, Merck, Bacto, Oxoid, ve Gelrite, bilinen bir armut anacı olan Quince A’nın köklenmesi üzerine incelenmiştir. Çalışmada kullanılan agar tipine ve konsantrasyonuna bağlı olarak farklı köklenme oranları oluşmuştur. En yüksek köklenme oranı 9 gL−1 Oxoid agar içeren ortamda kaydedilmiştir. Kök sayısı ve uzunluğu Bacto agar eklenen ortamlarda en iyi olarak belirlenmiş, özellikle 7 gL−1 konsantrasyonu öne çıkmıştır. Gelrite Quince A’nın köklenmesi için uygun bulunmamıştır. İlk çalışmada, Merck (7 gL−1), Bacto (7 gL−1), ve Oxoid (7 & 9 gL−1) agar konsantrasyonları en iyi olarak belirlenmiş, çalışmanın ikinci kısmında bu agar konsantrasyonlarına 0.5 gL−1 IBA veya 0.5 gL−1 NAA ilave edilmiştir. IBA kök sayısı ve kök uzunluğu üzerinde daha etkili bulunmuştur. Ekonomiklik açısından değerlendirildiğinde Merck agar (7 gL−1) öne çıkarken, Bacto agar (7 gL−1) Quince A’nın kök kalitesi bakımından en iyi sonuçları vermiştir

Keywords

Agar tipleri, Quince A, in vitro köklenme, NAA, IBA, maliyet analizi

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