Chickpea (Cicer arietinum L.) in vitro micropropagation

Amina Kadırı; Yamina Halfaouı; Louiza Bouabdallah; Zohra Ighılharız

Chickpea (Cicer arietinum L.) in vitro micropropagation

Year 2014, Volume: 1 Issue: Özel Sayı-1, 1304 - 1309, 01.03.2014

Amina Kadırı Yamina Halfaouı Louiza Bouabdallah Zohra Ighılharız

Abstract

This research was undertaken to determine chickpea in vitro micropropagation optimal conditions from preexisting meristems. Three genotypes; Zouaoui, ILC 483 and INRA 199 mature embryos and nodes were cultivated on Murashige and Sckoog (1962) medium without plant growth regulators and with NAA (Naphtyl acetic acid) /BAP (Benzyl amino purine) (0.5/4.5mg/l) and KIN (Kinetin) (0.5, 1mg/l).Explant morphogenetic response was recorded after one month incubation. Results were expressed as direct regeneration frequency (shoot and root development from embryos and axillary buds from nodes). Zouaoui genotype was more callogenic (42.57%) than INRA 199 (32.12%) and ILC 483 (23.43%). Among media, MS without hormones was suitable to induce preexisting meristems development (76.16%), whereas MS with NAA/BAP was favorable to callus formation (70.95%). Indirect buds formation was accomplished on MS added with 0.5mg/l KIN via callusing on mature embryos

Keywords

Chickpea, mature embryos, nodes, in vitro culture, callogenesis, buds formation

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