An Easy Assay Technique of Detection and Identification of Phytophthora Infestans (Mont.) De Bary From Seed Tubers Before Planting for Healthy Production of Potato

Year 2017, Volume: 4 Issue: 1, 63 - 69, 06.01.2017

Touseef Hussaın Birpal Sıngh Firoz Anwar

Abstract

The timely detection and appropriate
identification of causal agents associated with disease of crop plants or seeds
are considered to be the most important issue in formulating the management
strategies for plant diseases. This is particularly important for plant
diseases of a fungal nature, where disease-free planting materials is the only
effective way to restrict the disease. Beside this, morphological discrimination requires
special skill and the expertise of taxonomists or specialists and also time
consuming. To simplify the detection, end-point polymerase chain reaction (PCR)
assays were developed. Consensus sequences obtained from multiple alignments of
target genes, RAPD based methodology was used to design the SCAR maker for
rapid detection of P. infestans (amplified product 524bp). BLASTn
was also used for in silico specificity. No cross reactivity was
observed when primers were checked against other Phytophthora spp. The
described primer sets allowed accurate identification and detection of P.
infestans. All tests have multiple applications including screening of
healthy planting materials, breeding programs and disease diagnosis.

Keywords

P. infestans, potato seed tubers, direct PCR, diagnosis

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