Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze

Yıl 2018, Cilt: 5 Sayı: 2, 121 - 126, 06.05.2018

Melih Yılar Yusuf Bayar

https://doi.org/10.30910/turkjans.421344

Cited By: 1

Öz

This study was carried out to
determine the antifungal potential of Thymbra
spicata L. and Rosmarinus officinalis
L. (Lamiaceae) plant essential oils against the Monilinia fructigena Honey in Whetze pathogen which is one of the
major disease agents of soft seeds. The essential oils were dropped on a filter
paper adhered to the covers of petri dishes with a micropipette at the doses of
0 (Control), 0.5, 0.8, 1, 1.5, 2, 4, 8, 16 μl/petri dish. At the end of the
seven days incubation period, the diameters of the mycelium growth in petri
dishes were measured with an automatic caliper. As a result, T. spicata L. and R. officinalis plant essential oils were found to be statistically
significant on the pathogen. Depending on the increased dose, R. officinalis essential oil inhibited
the development of the M. fructigena mycelium and inhibited by 100% in 16 μl/petri dish. On the other hand, T. spicata essential oil influenced the mycelium development of the
pathogen more and inhibited it by 100% at the dose of 2 μl/petri
dish. As a result of dose-response study regarding T.
spicata and R. officinalis essential oils against M. fructigena  the LC₅₀
and LC₉₀ values were found to be 0.80-1.63 and 5.36-12.17,
respectively. These findings showed that T. spicata and R. officinalis essential oils may be an alternative to
synthetic fungicides for controlling M.
fructigena.

Anahtar Kelimeler

Thymbra spicata, Rosmarinus officinalis, Monilinia fructigena, essential oil, antifungal activity, biopescticide

Thymbra spicata L. ve Rosmarinus officinalis L. Uçucu Yağlarının Monilinia fructigena Honey in Whetze’ya Karşı Antifungal Aktivitesi

Öz

Bu
çalışma, yumuşak çekirdeklilerin önemli hastalık etmenlerden biri olan Monilinia
fructigena patojenine
karşı Thymbra spicata L. ve Rosmarinus officinalis L. (Lamiaceae)
bitki uçucu yağlarının antifungal potansiyelinin belirlenmesi amacıyla
yürütülmüştür. Uçucu yağlar petri kaplarının kapaklarına yapıştırılan filtre
kağıdına 0 (Kontrol),0.5, 0.8, 1, 1.5, 2, 4, 8, 16 μl/petri dozda mikropipetle
damlatılmıştır.  Yedi günlük inkubasyon
süresi sonunda petrilerdeki miselyum çapları otomatik kumpas ile ölçülmüştür.
Sonuçta T. spicata L. ve R. officinalis bitki uçucu yağları
patojen üzerine istatistiki olarak önemli derece etkili bulunmuştur. R. officinalis uçucu yağı artan doza
bağlı olarak M. fructigena miselyum
gelişimini engellemiş olup 16 μl/petri kabı dozda %100 engellemiştir. T. spicata uçucu yağı ise patojenin
miselyum gelişimi üzerine daha fazla etki göstermiş; 2 μl/petri
kabı dozda %100 inhibe etmiştir. T.
spicata ve R. officinalis uçucu yağlarının M. fructigena karşı
doz-etki çalışması sonucunda LC₅₀ ve LC₉₀ değerleri
sırasıyla, 0.80-1.63 ve 5.36-12.17 olarak bulunmuştur. Bu bulgular, T. spicata ve R. officinalis uçucu yağlarının,  M.
fructigena kontrolünde sentetik fungusitlere alternatif olabileceğini
göstermiştir.

Anahtar Kelimeler

Thymbra spicata, Rosmarinus officinalis, Monilinia fructigena, uçucu yağ, antifungal aktivite, biyopestisit

Kaynakça

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