Effect of PEG Induced Drought Stress on Germination and Seedling Traits of Maize (Zea mays L.) Lines

Year 2019, Volume: 6 Issue: 2, 196 - 205, 22.04.2019

Manu M. Magar , Atit Parajuli Bindeshwor P. Sah Jiban Shrestha Binesh M. Sakh Keshab B. Koirala Shambhu P. Dhital

https://doi.org/10.30910/turkjans.556607

Cited By: 5

Abstract

Maize is the staple food crop in hilly areas of Nepal.
Drought stress is one of the most important crop growth limiting factors
leading to lower crop productivity in these areas. Maize seedlings are
susceptible to drought stress. Nine lines (Arun-2, NML-1, Rampur Composite,
RL-100, RL-105, Rl-106, RL-197, RML-18 and RML-8) of maize obtained from
National Maize Research Program, Rampur, Chitwan, Nepal to study their
tolerance to drought stress. An experiment was designed to study their drought
tolerance by subjecting to four levels of osmotic potential (0, -5, -10 and -15
bar) induced by polyethylene glycol (PEG) 6000 in two factor completely
randomized design with three replication at Biotechnology Division, National
Agricultural Research Institute, Khumaltar, Lalitpur, Nepal. Effects of
different levels of PEG 6000 were found significant on all traits studied viz.
Germination rate (%), Speed of germination, Root length, Shoot length, Root
Shoot ratio, Seedling length, Seedling fresh weight, Seedling dry weight and
Vigor index. Line Arun-2 was recorded as best performer followed by Rampur
Composite and RL-105 for all the traits analyzed. Lines RL-100, RL-106, RL-197,
RML-18 and RML-8 showed poor performance under the same levels of PEG induced
drought stress. Furthermore, the results also showed that maize manifests
better genetic expression such as drought tolerance under severe moisture
stress conditions in soil. Therefore, Lines Arun-2, Rampur Composite and RL-
105 are suggested for varietal improvement program for drought stress conditions.

Keywords

Drought stress, maize, osmotic potential, PEG, seedling parameters

Effect of PEG Induced Drought Stress on Germination and Seedling Traits of Maize (Zea mays L.) Lines

Abstract

Maize is the staple food
crop in hilly areas of Nepal. Drought stress is one of the most important crop
growth limiting factors leading to lower crop productivity in these areas.
Maize seedlings are susceptible to drought stress. Nine lines (Arun-2, NML-1,
Rampur Composite, RL-100, RL-105, Rl-106, RL-197, RML-18 and RML-8) of maize
obtained from National Maize Research Program, Rampur, Chitwan, Nepal to study
their tolerance to drought stress. An experiment was designed to study their
drought tolerance by subjecting to four levels of osmotic potential (0, -5, -10
and -15 bar) induced by polyethylene glycol (PEG) 6000 in two factor completely
randomized design with three replication at Biotechnology Division, National
Agricultural Research Institute, Khumaltar, Lalitpur, Nepal. Effects of
different levels of PEG 6000 were found significant on all traits studied viz.
Germination rate (%), Speed of germination, Root length, Shoot length, Root
Shoot ratio, Seedling length, Seedling fresh weight, Seedling dry weight and
Vigor index. Line Arun-2 was recorded as best performer followed by Rampur
Composite and RL-105 for all the traits analyzed. Lines RL-100, RL-106, RL-197,
RML-18 and RML-8 showed poor performance under the same levels of PEG induced
drought stress. Furthermore, the results also showed that maize manifests
better genetic expression such as drought tolerance under severe moisture
stress conditions in soil. Therefore, Lines Arun-2, Rampur Composite and RL-
105 are suggested for varietal improvement program for drought stress conditions.

Keywords

Drought stress, maize, osmotic potential, PEG, seedling parameters

References

• Ahmad, S., Ahmad, R., Ashraf, M.Y., Ashraf, M., Waraich, E.A. 2009. Sunflower (Helianthus annuus L.) response to drought stress at germination and growth stages. Pakistan Journal of Botany, 41: 647-654.
• Álvarez-Iglesias, L., de la Roza-Delgado, B., Reigosa, M.J., Revilla, P., Pedrol, N. 2017. A simple, fast and accurate screening method to estimate maize (Zea mays L.) tolerance to drought at early stages. Maydica, 62: 3-24.
• Avramova. V., Abd Elgawad, H., Zhang, Z., Fotschki, B., Casadevall, R., Vergauwen, L., Knapen, D., Taleisnik, E., Guisez, Y., Asard, H., Beemster, G.T.S. 2015. Drought Induces Distinct Growth Response, Protection and Recovery Mechanisms in the Maize Leaf Growth Zone. Plant Phyiology, 169: 1382-1396.
• Ayalew, H., Ma, X., Yan, G. 2014. Screening Wheat (Triticum spp.) Genotypes for Root Length under Contrasting Water Regmies: Potential Sources of Variability for Drought Resistance Breeding. Journal of Agronomy and Crop Science, 201(3): 189-194.
• Blum, A. 2011. Drought resistance – is it really a complex trait?. Functional Plant Biology, 38: 753-757.
• Comas, L., Becker, S., Cruz, V.M.Z., Byrne P.F., Dierig, D.A. 2013. Root Traits Contributing To Plant Productivity under Drought. Frontiers in Plant Science, 4.
• Dhanda, S.S., Sethi, G.S., Behl, B.K. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science, 190: 6-12.
• Edmeades, G.O., Bänziger, M., Campos, H., Schussler, J. 2006. Improving tolerance to abiotic stresses in staple crops: a random or planned process. Plant Breeding: The Arnel R. Hallauer International Symposium, pp. 293-309.
• Farooq, J., Khaliq, I., Ali, M.A., Kashif, M., Rehman, A.,Naveed, M., Ali, Q., Nazeer, W., Farooq, A. 2011. Inheritance pattern of yield attributes in spring wheat at grain filling under different temperature regimes. Australian Journal of Crop Science, 5(13): 1745-1753.
• Guoxiong, C., Krugman, T., Fahima, T., Korol, A.B., Nevo, E. 2002. Comparative study on morphological and physiological traits related to drought resistance between xeric and mesic Hordeum spontaneum lines in Isreal. Barley Genetics Newsletter, 32: 22-33.
• Hanan, E.D. 2007. Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Advances in Biological Research, 1: 40-48.
• Huang, Q., Zhao, Y., Liu, C., Zou, X., Cheng, Y. Fu, G., Xu, J., Zhang, X., Lu, G. 2015. Evaluation of and selection criteria for drought resistance in Chinese semi-winter rapeseed varieties at different developmental stages. Plant Breeding, 134(5): 542-550.
• ISTA (International Seed Testing Association). 1996. International rules for seed testing rules. Seed Science and Technology. 24, Supplement.155-202.
• Kauser, R., Athar, H.U.R., Ashraf, N. 2006. Chlorophyll flurescence: A potential indicator for rapid assessment of water stress tolerance in canola (Brassica napus L.). Pakistan Journal of Botany, 38: 1501-1509.
• Khan, N.H., Ahsan, M., Naveed, M., Sadaqat, H.A., Javed, I. 2016. Genetics of drought tolerance at seedling and maturity stages in Zea maysL.. Span Journal of Agricultural Research, 14(3): e0705. http://dx.doi.org/10.5424/sjar/2016143-8505.
• Khayatnezhad, M., Gholamin, R., Jamaati-e-Somarin, S.H., Zabihi-Mahmoodabad, R. 2010. Effects of peg stress on corn cultivars (Zea mays L.) at germination stage. World Applied Sciences Journal, 11(5): 504-506.
• Khodarahmpour, Z. 2011. Effect of drought stress induced by polyethylene glycol (PEG) on germination indices in corn (Zea mays L.) hybrids. African Journal of Biotechnology, 10(79): 18222-18227.
• Kulkarni, M., Deshpande, U. 2007. In Vitro screening of tomato genotypes for drought resistance using polyethylene glycol. African Journal of Biotechnology, 6: 691-696.
• Kumar, A.P., Reddy, N.N., Lakshmi, J.N. 2017. PEG Induced screening for drought tolerance in tomato genotypes. International Journal of Current Microbiology and Applied Sciences, 6(7): 168-181.
• Langridge, P., Reynolds, M.P. 2015. Genomic tools to assist breeding for drought tolerance. Current Opinion in Biotechnology, 32: 130-135.
• Lobell, D.B., Roberts, M.J., Schlenker, W., Braun, N., Little, B.B., Rejesus, R.M., Hammer, G.L. 2014. Greater sensitivity to drought accompanies maize yield increase in the U.S. Midwest. Science, 344: 516-519.
• MoAD. 2013. Statistical Information on Nepalese Agriculture, 2012/13. Ministry of Agricultural Development, Singh Durbar, Kathmandu, Nepal.
• Mostafavi, K.H., SadeghiGeive, H., Dadresan, M., Zarabi, M. 2011. Effects of drought stress on germination indices of corn hybrids (Zea mays L.). International Journal of Agricultural Science, 1(2): 10-18.
• Mut, Z., Akay, H., Aydin, N. 2010. Effects of seed size and drought stress on germination and seedling growth of some oat genotypes (Avena sativa L.). African Journal of Agricultural Research, 5: 1101-1107.
• Obidiegwu, J.E., Bryan, G.J., Jones, H.G., Prashar, A. 2015. Coping with Drought: Stress and Adaptive Responses in Potato and Perspectives for Improvement. Frontiers in Plant Science, 6.
• Partheeban, C., Chandrasekhar, C.N., Jeyakumar, P., Ravikesavan, R., Gnanam, R. 2017. Effect of PEG induced drought stress on seed germination and seedling characters of maize (Zea mays L.) genotypes. International Journal of Current Microbiology and Applied Sciences, 6(5): 1095-1104.
• Petcu, E., Martura, T., Ciocazanu, I., Iordan, H.L., Badut, C., Urechean, V. 2018. The Effect of water stress induced with PEG solution on maize seedlings. Romanian Agricultural Research, No. 35, DOI: 2067-5720 rar 2018-121.
• Queiroz,M.S., Oliveira,C.E.S., Steiner, F., Zuffo, A.M., Zoz, T., Vendruscolo, E.P., Silva, M.V., Mello, B.F.F.R., Cabra, R.C., Menis, F.T. 2019. Drought stresses on seed germination and early growth of maize and sorghum. Journal of Agricultural Science, 11(2): 310-318.
• Ribaut, J.M., Betran, J., Monneveux, P., Setter, T. 2009. Drought Tolerance in Maize. In: Bennetzen, J. & Hake S. (Eds.) Handbook of Maize: It’s Biology., pp. 311-344. Springer, New York.
• Scott, S.J., Jones, R.A., Williams, W.A. 1984. Review of data analysis methods for seed germination. Crop Science, 24: 1192-1199.
• Shamim, F., Saqlan, S.M., Athar, H-u-R., Waheed, A. 2014. Screening and selection of tomato genotypes/cultivars for drought tolerance using multivariate analysis. Pakistan Journal of Botany, 46: 1165-1178.
• Shatpathy, P., Kar, M., Dwibedi, S.K., Dash, A. 2018. Seed priming with salicylic acid improves germination and seedling growth of rice (Oryza sativa L.) under PEG-6000 induced water stress. International Journal of Current Microbiology and Applied Sciences, 7(10): 907-924.
• Singh, B., Usha, K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulator, 39: 137-141.
• Spielmeyer, W., Hyles, J., Joaquim, P., Azanza, F., Bonnet, D., Ellis, M.E., Moore, C., Richards, R.A. 2007. A QTL on chromosome 6A in bread wheat is associated with longer coleoptiles, greater seedling vigor and final plant height. Theoretical and Applied Genetics, 115: 59-66.
• Steel, R.G.D., Torrie, J.H., Dickey, D.A. 1997. Principles and Procedures of Statistics; A Biometrical Approach. 3. Boston: McGraw-Hill.
• Tripathi, M.P., 2012. Development of Drought and Low N Stress Tolerant Maize Cultivars for Terai and Mid hills of Nepal. Annual Report of National Maize Research Program 2011/12, Rampur, Chitwan, Nepal, pp. 103-107.
• Tsago, Y., Andargie, M., Takele, A. 2014. In vitro selection of sorghum (Sorghum bicolor (L.) Moench) for polyethylene glycol (PEG) induced drought stress. Plant Science Today, 1(2): 62-68.
• Zhan, A., Schneider, H., Lynch, J.P. 2015. Reduced Lateral Root Branching Density Improves Drought Tolerance in Maize. Plant Physiology, 16.