Investigation of Antimicrobial Effects of Silver Nanoparticles (AgNPs) Synthesized on Polygonum cognatum Meissn. and Fungus Environment

Yıl 2020, Cilt: 7 Sayı: 1, 221 - 230, 25.01.2020

Nevcihan Gürsoy Sezai Elagöz Evren Gölge

https://doi.org/10.30910/turkjans.680075

Öz

In this study, antimicrobial effects of silver nanoparticles (AgNPs) synthesized on Madımak (Polygonum cognatum Meissn.) and A. alternata were investigated. The antimicrobial activity was investigated by using bacterial species such as Staphyloccus auerus, Enterecoccus fecalis, Escherichia coli and fungus species such as Aspergillus niger, Penicillium chrysogenum and Fusarium oxysporium, which are common in foods and caused serious health problems as a result of consumption. UV-Vis Spectrophotometry, Atomic Force Microscope (AFM) and High Contrast Scanning Electron Microscope (CCTEM) were used for particle characterization of synthesized AgNPs. In the statistical evaluation of Kirby Bauer Disk Diffusion test results, which were applied to determine the antimicrobial efficacy, the data were expressed as mean ± standard error and analyzed by using SPSS V22 software. AgNPs synthesized on Madımak and A. alternata showed no antifungal effect on F. oxysporum. However, it has been found that it has a high antifungal effect on other fungi species but this effect has no superiority over Amphotericin B agent. It was determined that AgNPs obtained from both syntheses had antibacterial effect on all bacterial species studied. However, it was found that AgNPs synthesized on A. alternata had a stronger antibacterial activity on S. aureus than AgNPs synthesized on Madımak and Ampicillin, an antibacterial agent (p <0.05).

Anahtar Kelimeler

Madımak, A. alternata

Polygonum cognatum Meissn. Ve Funguslu Ortamda Sentezlenen Gümüş Nanopartiküllerinin (AgNP) Antimikrobiyal Özelliklerinin Araştırılması

Öz

Bu çalışmada, Madımak (Polygonum cognatum Meissn.) ve A. alternata ve üzerinde sentezlenen gümüş nanopartiküllerinin (AgNP) antimikrobiyal etkileri araştırılmıştır. Antimikrobiyal etkinin araştırılmasında patojen bakteri suşlarından gıdalarda yaygın olarak bulunan ve tüketimleri sonucunda ciddi sağlık sorunlarına neden olan Staphyloccus auerus, Enterecoccus fecalis, ve Escherichia coli bakteri türleri ile Aspergillus niger, Penicillium chrysogenum ve Fusarium oxysporium fungus türleri kullanılmıştır. Sentezlenen AgNP’lerin karakterizasyonunda UV-Vis Spektrofotometri Atomik Güç Mikroskobu (AFM) ve Yüksek Kontrastlı Taramalı Elektron Mikroskobu (CCTEM) kullanılmıştır. Antimikrobiyal etkinliğin belirlenmesi amacıyla uygulanan Kirby Bauer Disk Difüzyon testi verilerinin istatistiki değerlendirmelerinde veriler ortalama ± standart hata olarak ifade edilmiş ve SPSS Ver. 22 yazılım programı kullanılarak analiz edilmiştir. Madımak ve A. alternata üzerinde sentezlenen AgNP’leri sadece F. oxysporum’da antifungal etki göstermemiştir. Bununla birlikte diğer fungus türleri üzerinde yüksek bir antifungal etkiye sahip olduğu ancak bu etkinin antifungal ajan Amphotericin B’ye göre bir üstünlüğünü olmadığı saptanmıştır. Her iki sentez sonucunda elde edilen AgNP’lerin çalışılan tüm bakteri türlerinde antibakteriyel etkiye sahip olduğu belirlenmiştir. Ancak A. alternata üzerinde sentezlenen AgNP’lerinin S. aureus üzerinde hem Madımak üzerinde sentezlenen AgNP’lerden hem de Ampicilin antibakteriyel ajanından daha güçlü bir antibakteriyel etkinliği olduğu tespit edilmiştir (p<0.05).

Anahtar Kelimeler

Madımak, A. alternata

Kaynakça

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