İn Vivo Ve İn Vitro Koşullarında Bazı Alkanna Taksonların Sekonder Metabolit İçerikleri Ve Antioksidan Aktiviteleri

Year 2020, Volume: 7 Issue: 3, 618 - 626, 20.07.2020

Cennet Yaman , Serkan Uranbey , Muhammet Er , Dilek Başalma

https://doi.org/10.30910/turkjans.672572

Cited By: 2

Abstract

Bu çalışmada in vivo koşullarda yetişmiş ve in vitro koşullarda büyütülen Alkanna orientalis (L.) Boiss. var. orientalis ve endemik Alkanna sieheana Rech. Fil. taksona ait bitki örneklerinde toplam alkanin/şikonin (A/Ş), toplam fenolik ve flavonoid içerikleri ile DPPH aktivitelerinin belirlenmesi amaçlanmıştır. Her iki taksona ait doğadan toplanan tohumlar in vitro koşullarda farklı büyüme düzenleyicileri içeren Murashige ve Skoog (MS) besin ortamında sekonder metobolit üretimine uygun çoğaltım ve rejenerasyon ortamında kültüre alınmıştır. A. orientalis‘de en yüksek sürgün sayısı (6.53 adet/bitki) 0.25 mg L-1 BAP, 0.5 mg L-1 KIN, 1.0 mg L-1 IAA , A. sieheana‘da ise 5.57 adet/bitki ile 0.25 mg L-1 BAP, 0.5 mg L-1 KIN, 1.0 mg L-1 IBA içeren besin ortamında elde edilmiştir. Her iki taksonda en yüksek ekstrakt verimi in vivo dan toplanan örneklerde saptanmıştır. A. orientalis taksonunda en yüksek toplam fenolik içerik 172.80 mg GAE g-1 ekstrakt ile in vivo koşullarında kök ekstraktında, A. sieheana için ise 122.99 mg GAE g-1 ekstrakt ile in vivo koşullarda herba ekstraktında gözlenmiştir. Her iki takson için de en yüksek toplam flavonoid içerik yine in vivo koşularındaki ekstraklarda belirlenmiştir. A. sieheana türünde en yüksek toplam A/Ş içeriği (61.45 µg g-1 ekstrakt) in vivo koşullarındaki köklerinden elde edilmiş ve A. orientalis köklerinden 23 kat daha fazla olduğu saptanmıştır. En yüksek radikal kovucu aktivite ise A. orientalis için 1000 µg ml-1 konsantrasyonda in vivo koşullarında köklerden, A. sieheana için 1500 µg ml-1 konsantrasyonda yine in vivo koşularındaki herba kısmında belirlenmiştir.

Keywords

Alkanna, Alkanin/Şikonin, DPPH, Fenolik, Flavonoid

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