Molecular Phylogenetic Analysis Of Morkaraman Sheeps In Bingol Region

Yıl 2021, Cilt: 8 Sayı: 1, 171 - 178, 23.01.2021

Oğuz Ağyar Emin Özköse Mehmet Sait Ekinci İsmail Akyol

https://doi.org/10.30910/turkjans.642421

Öz

In the present study, we report mitochondrial DNA (mtDNA) 16S rRNA gene sequence analysis of the Morkaraman ewe, (Ovis aries) was investigated. In domestic sheep, the 16S rRNA gene is ca 1574 bp in length. Partial fragment of 536 bp 16S rRNA gene of mtDNA was amplified by using designed primers from published data of Sheep sequences derived from National Center for Biotechnology Information (NCBI) web server (ncbi.nlm.nih.gov/NC_001941.1). Homology of the sequences obtained from the present work were performed by using BLAST program at NCBI. Obtained 16S rRNA gene sequences were submited to the GenBank database with accession numbers, KU686952.1, KU686953.1, KU686954.1, KU686955.1, KU686956.1, KU686957.1, KU686958.1, KU686959.1, KU686960.1, KU686961.1 and KU686962.1, respectively. According to nucleotide differences of mtDNA 16S rRNA gene region 10 polymorphic regions and 8 haplotypes were detected in Turkish domestic sheep Morkaraman (n=11). The nucleotide composition averages of all the sequences was 32.1% Adenine (A), 24.2% Thymine (T), 22.2% Cytosine (C) and 21.5% Guanine (G); G+C was 43.7%. DNA polymorphism based on 16S rRNA gene sequences, haplotype diversity (Hd) and nucleotide diversity (π) were found to be 0,9273±0,00442, and 0,00427±0,00125, respectively. Based on gene sequences information, in sheeps, mtDNA haplotypes, the phylogenetic relationship among mtDNA polymorphism, haplotypes and relationship between native sheep breeds and foreign sheep breeds were determined and discussed. Thus, sequence analysis of mitochondrial 16S rRNA gene can be used as a tool for phylogenetic analysis of Morkaraman sheep.

Anahtar Kelimeler

Genetic Diversity,, Morkaraman (Ovis aries),, Phylogenetic,, mtDNA,, 16S rRNA

Molecular Phylogenetic Analysis Of Morkaraman Sheeps In Bingol Region

Öz

Bu çalışmada, Morkaraman koyunlarının (Ovis aries) filogenetik ilişkileri mitokondriyal DNA (mtDNA) 16S rRNA gen dizisi analizi ile gerçekleştirilmiştir. Evcil koyunlarda, 16S rRNA geni 1574 baz çifti (bç) uzunluğundadır. Çalışmamızda mtDNA 16S rRNA gen bölgesi hedeflenerek, Ulusal Biyoteknoloji Bilgi Merkezi (NCBI) alınan örnek sekansla (NC_001941.1) tasarlanan primerler ile, 536 bç’lik sekans ürünleri elde edilmiştir. Elde edilen diziler için homoloji araştırması, NCBI'de BLAST kullanılarak yapılmıştır. Çalışma sonunda elde ettiğimiz 16S rRNA gen bölgesi dizi bilgileri GenBank veri tabanına sırası ile bu erişim numaralarıyla yer almaktadır; Sırasıyla KU686952.1, KU686953.1, KU686954.1, KU686955.1, KU686956.1, KU686957.1, KU686958.1, KU686959.1, KU686960.1, KU686961.1 ve KU686962.1. Morkaraman koyun örnekleri (n=11) üzerinde yapılan analizler sonunda, mtDNA 16S rRNA gen bölgesi sekanslarından nükleotit farklılıkları bakımından 10 polimorfik bölge ve 8 haplotip bölge tespit edilmiştir. Tüm dizilerin nükleotit kompozisyon ortalamaları % 32.1 Adenin (A), % 24.2 Timin (T), % 22.2 Sitosin (C) ve % 21.5 Guanin (G); G + C % 43.7 olarak bulundu. 16S rRNA gen dizileri, haplotip çeşitliliği (Hd) ve nükleotit çeşitliliğine (π) dayanan DNA polimorfizmi sırasıyla 0.9273 ± 0.00442 ve 0.00427 ± 0.00125 olarak bulunmuştur. Gen dizileri bilgisine dayanarak, koyunlarda, mtDNA haplotipleri, mtDNA polimorfizmi, haplotipleri arasındaki filogenetik ilişkiler ile belirlenen yerli ve yabancı koyun ırkları arasındaki filogenetik ilişkileri belirlenmiştir.

Anahtar Kelimeler

Filogenetik, Genetik Çeşitlilik, Morkaraman (Ovis aries)

Kaynakça

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