MICROPROPAGATION OF ORIENTAL ARBORVITAE (Thuja orientalis L.) VIA EMBRYO CULTURE

Year 2021, Volume: 8 Issue: 2, 381 - 387, 23.04.2021

Mehmet Sezgin , Mustafa Kahya

https://doi.org/10.30910/turkjans.853339

Abstract

Oriental arborvitae (Thuja orientalis L.), which belongs to the Cupressaceae family, is an important forest tree species for timber and wood products with its use in landscaping. However, its medicinal use puts oriental arborvitae in the class of very important plants. In the study, very important results have been obtained by propagation with the embryo culture technique, which is one of the important techniques of plant biotechnology. Along with different doses of sucrose, Woody Plant Medium (WPM) is added with doses of 6-benzyladenine (BA) (0, 1, and 2 mg l-1) and 2,4-Dichlorophenoxy acetic acid (2,4-D) or Indole-3-butyric acid (IBA) (0, 0.5, and 1 mg l-1) were combined and added to the medium. In the medium, 30 g l-1 sucrose was added to the BA (1 mg l-1) + IBA (0.5 mg l-1) growth regulator combination (BDM), and the germination success was 100%, while the maximum shoot length (4.8 cm) and root number (7.6). In the medium 45 g l-1 sucrose was added to BA (1 mg l-1) + 2,4-D (0.5 mg l-1) BDM combination, maximum root length (2.4 cm) was determined. 69% of the plants with acclimatization have adapted to external conditions.

Keywords

Acclimatization,, Embryo culture,, Thuja orientalis L.,, Tissue culture,, Sucrose,

Doğu Mazısının (Thuja orientalis L.) Embriyo Kültürü İle Çoğaltımı

Abstract

Cupresseaceae familyasına ait Doğu mazısı (Thuja orientalis L.), peyzaj açısından kullanımıyla birlikte kereste ve odun üretimi için önemli bir orman ağacı türüdür. Bununla birlikte tıbbi olarak kullanımı mazıyı oldukça önemli bitkiler sınıfına sokmaktadır. Çalışmada, bitki biyoteknolojisinin önemli tekniklerinden biri olan embriyo kültürü tekniği ile çoğaltarak oldukça önemli sonuçlar elde edilmiştir. Sakarozun farklı dozları ile birlikte, Woody Plant Medium (WPM) temel besin ortamına 6-benziladenin (BA)’in (0, 1, ve 2 mg l-1) dozları ile 2,4-Diklorofenoksi asetik asit (2,4-D) veya Indol-3-bütirik asit (IBA)’in (0, 0.5 ve 1 mg l-1) dozları kombine edilerek besin ortamına ilave edilmiştir. BA (1 mg l-1) + IBA (0.5 mg l-1) büyüme düzenleyici madde (BDM) kombinasyonuna 30 g/L sakaroz ilave edilmiş ortamda çimlenme başarısı %100 oranında meydana gelirken, maksimum sürgün uzunluğu (4.8 cm) ve kök sayısı (7.6) olarak elde edilmiştir. BA (1 mg l-1) + 2,4-D (0.5 mg l-1) BDM kombinasyonuna 45 g/L sakaroz eklenmiş ortamda ise maksimum kök uzunluğu (2.4 cm) belirlenmiştir. Aklimitizasyon uygulaması yapılan bitkilerden %69’u dış koşulara uyum göstermiştir.

Keywords

Aklimitizasyon,, Doku kültürü,, Embriyo kültürü,, Thuja orientalis L.,, Sakaroz,

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