Research Article
Erzurum Olur Yöresinden Toplanan Berberis vulgaris L. Meyvelerinin Antioksidan Aktivitelerinin Belirlenmesi
Abstract
Bu çalışma Berberis vulgaris L. meyve ekstraklarında toplam fenolik ve flavonoid madde miktarı ve antioksidan aktivitelerinin belirlenmesi amacıyla yapılmıştır. Folin-Ciocalteu ve alüminyum nitrat yöntemleri kullanılarak toplam fenolik ve flavonoid madde miktarları belirlendi. Hekzan ve metanol ekstraktında fenolik madde miktarı sırasıyla 33,64±1,86 mg GAE g-1 ve 85,98±1,59 mg of GAE g-1, flavonoid madde miktarı ise 94,74±2,59 mg KE g-1 ve 64,74±1,86 mg KE g-1 olarak belirlendi. DPPH• radikal giderme aktivitelerinin, fenolik madde miktarının çok olduğu metanol ekstraklarında, Fe3+-Fe2+ indirgeme kapasitesinin ise flavonoid madde miktarı fazla olan hekzan ekstraklarında daha yüksek olduğu belirlenmiştir. Bulduğumuz sonuçlara göre B. vulgaris’in gıda takviyesi ve doğal antioksidan kaynağı olarak değerlendirilebileceği kanaatine varılmıştır.
Keywords
Supporting Institution
Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi
Project Number
SYO.A4.20.002
Thanks
Bu çalışma Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje Numarası: SYO.A4.20.002). Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimine ve makalede kullandığımız fotoğraflar için Sayın Ergin ERGÜN’e teşekkür ederiz.
References
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- Alavia, N., Mazloumzadeh, S.M. 2012. Effect of harvesting and drying methods of seedless barberry on some fruit quality. J. Saudi Soc. Agric. Sci. 11: 51-55.
- Arslanoglu, S.F., Ayna, O.F. 2019. Anadolu Cografyasında Yayılış Gösteren Berberis Türleri ve Geleneksel Kullanımı. International Journal of Life Sciences and Biotechnology. 2(1): 36-42.
- Bakmohamadpor, M., Javadi A., Azadmard-Damirchi, S., Jafarizadeh-Malmiri, H. 2021. Effect of barberry (Berberis vulgaris) fruit powder on the quality and shelf life stability of puffed corn extrude. NFS Journal, 22: 9-13.
- Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181:1199-1200.
- El Fakir, L., Bouothmany, K., Alotaibi, A., Bourhia, M., Ullah, R., Zahoor, S., El Mzibri, M., Gmouh, S., Alaoui, T., Zaid, A., Benbacer, L. 2021. Antioxidant and understanding the anticancer properties in human prostate and breast cancer cell lines of chemically characterized methanol extract from Berberis hispanica Boiss. & Reut. Appl. Sci. 11(8): 3510.
- Erlund, I., Koli, R., Alfthan, G., Marniemi, J., Puukka, P., Mustonen, P., Mattila, P., Jula, A. 2008. Favorable effects of berry consumption on platelet function, blood pressure, and HDL cholesterol. The Am. J. of Clin. Nutrition. 87(2): 323-331.
- Eroğlu, A.Y., Çakır, Ö., Sağdıç, M., Dertli E. 2020. Bioactive characteristics of wild Berberis vulgaris and Berberis crataegina fruits. Journal of Chemistry. Article ID 8908301.
- Falahi, E., Ahmadvand, H., Moghadam, E.H., Roosta, S. 2019. Antioxidant properties of some traditional ıranian edible fruits growing wild in lorestan province in the western Iran. 4(2): 76-81.
- Foo, L.Y., Porter, L.J., 1981. The structure of tannins of some edible fruits. Journal Science Food Agricaltural, 32: 711-716.
- Gundogdu, M. 2013. Determination of antioxidant capacities and biochemical compounds of Berberis vulgaris L. Fruits. Advances in Environmental Biology, 7(2):344-348.
- Hadi, A., Arab, A., Ghaedi, E., Rafie, N., Miraghajani, M., Kafeshani, M. 2019. Barberry (Berberis vulgaris L.) is a safe approach for management of lipid parameters: A systematic review and meta-analysis of randomized controlled trials. Complement. Ther. Med. 43: 117-124.
- Hanachi, P., Golkho, S.H. 2009. Using HPLC to determination the composition and antioxidant activity of Berberis vulgaris. European Journal of Scientific Research. 29(1):47-54.
- Hassanpour, H., Alizadeh, S. 2016. Evaluation of phenolic compound, antioxidant activities and antioxidant enzymes of barberry genotypes in Iran. Sci Hortic. 200: 125-130.
- Larson, R.A. 1988. The antioxidants of higher plants. Phytochemistry. 27: 969-978.
- Marianne, L., Engelhart, M.D. 2002. High ıntakes of antioxidant vitamins C and E may lower the risk of alzheimer’s disease. Journal of the American Medical Association 287 (24): 3223.
- Marja, P.K., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kajula T.S., Heinonen, M. 1999. Antioksidant Activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry (47): 3954-3962.
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- Moein, M., Sabahi, Z., Salim, H. 2020. A glance at Berberis integerimma pharmacological effects and its active constituents. Trends in Pharmaceutical Sciences. 6(1): 1-10.
- Moreno, M.I.N., Isla, M.I., Sampietro, A.R. and Vattueno, M.A. 2000. Comparison of the free radical scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71:109-114.
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- Oyaizu, M. 1986. Study on products of Browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315.
- Özkan K., Açıkgöz, Z. 2007. Kanatlı kümes hayvanlarının beslenmesi. 1.Baskı, Hasad Yayıncılık, İstanbul.
- Sarla, S., Subhash, C., Alok, K.S. 2011. Berberis asiatica future based excellent fruit in nutritional profile, antimicrobial and antioxidant ingredients. International research journal of pharmacy. 2(12): 213-216.
- Shamsa, F., Ahmadiani, A., Khosrokhavar, R. 1999. Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. Journal of Ethnopharmacology. 64, 161-166.
- Sharifi, F., Poorakbar, L. 2015. The survey of antioxidant properties of phenolic compounds in fresh and dry hybrid Barberry fruits (Berberis integerrima× vulgaris). Cumhuriyet Science Journal. 36(3): 1609-1617.
- Slinkard, K., Singleton, V.L. 1977. Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28:49-55.
- Thangapazham, R.L., Sharma, A., Maheshwari, R.K. 2006. Multiple molecular targets in cancer chemoprevention by curcumin. The AAPS Journal. 8(3): 443.
- Zargari, A. 1983. Medicinal Plants, Tehran University Press, Tehran 1, 68.
Year 2021,
Volume: 8 Issue: 4, 1028 - 1034, 24.10.2021
Abstract
This study was carried out to determine the total amount of phenolic and flavonoid substances and antioxidant activities in Berberis vulgaris L. fruit extracts. Total phenolic and flavonoid substances were determined by using Folin-Ciocalteu and aluminum nitrate methods. The amount of phenolic substance in the hexane and methanol extract was determined as 33.64±1.86 mg GAE g-1 and 85.98±1.59 mg of GAE g-1 respectively, and the amount of flavonoid substance was determined as 94.74±2.59 mg QE g-1 and 64.74±1.86 mg QE g-1 respectively. It was determined that DPPH• radical scavenging activities were higher in methanol extracts with a high amount of phenolic substances, while the reducing capacity of Fe3+-Fe2+ was higher in hexane extracts with a higher amount of flavonoid substances. According to the results we found, it was concluded that B. vulgaris can be considered as a food supplement and a source of natural antioxidants.
Project Number
SYO.A4.20.002
References
- Akbulut, M., Çalışır, S., Marakoglu, T., Çoklar, H. 2009. Some physicomechanical and nutritional properties of Berberis vulgaris L. fruits. Journal of Food Process Engineering. 32: 479-511.
- Alavia, N., Mazloumzadeh, S.M. 2012. Effect of harvesting and drying methods of seedless barberry on some fruit quality. J. Saudi Soc. Agric. Sci. 11: 51-55.
- Arslanoglu, S.F., Ayna, O.F. 2019. Anadolu Cografyasında Yayılış Gösteren Berberis Türleri ve Geleneksel Kullanımı. International Journal of Life Sciences and Biotechnology. 2(1): 36-42.
- Bakmohamadpor, M., Javadi A., Azadmard-Damirchi, S., Jafarizadeh-Malmiri, H. 2021. Effect of barberry (Berberis vulgaris) fruit powder on the quality and shelf life stability of puffed corn extrude. NFS Journal, 22: 9-13.
- Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181:1199-1200.
- El Fakir, L., Bouothmany, K., Alotaibi, A., Bourhia, M., Ullah, R., Zahoor, S., El Mzibri, M., Gmouh, S., Alaoui, T., Zaid, A., Benbacer, L. 2021. Antioxidant and understanding the anticancer properties in human prostate and breast cancer cell lines of chemically characterized methanol extract from Berberis hispanica Boiss. & Reut. Appl. Sci. 11(8): 3510.
- Erlund, I., Koli, R., Alfthan, G., Marniemi, J., Puukka, P., Mustonen, P., Mattila, P., Jula, A. 2008. Favorable effects of berry consumption on platelet function, blood pressure, and HDL cholesterol. The Am. J. of Clin. Nutrition. 87(2): 323-331.
- Eroğlu, A.Y., Çakır, Ö., Sağdıç, M., Dertli E. 2020. Bioactive characteristics of wild Berberis vulgaris and Berberis crataegina fruits. Journal of Chemistry. Article ID 8908301.
- Falahi, E., Ahmadvand, H., Moghadam, E.H., Roosta, S. 2019. Antioxidant properties of some traditional ıranian edible fruits growing wild in lorestan province in the western Iran. 4(2): 76-81.
- Foo, L.Y., Porter, L.J., 1981. The structure of tannins of some edible fruits. Journal Science Food Agricaltural, 32: 711-716.
- Gundogdu, M. 2013. Determination of antioxidant capacities and biochemical compounds of Berberis vulgaris L. Fruits. Advances in Environmental Biology, 7(2):344-348.
- Hadi, A., Arab, A., Ghaedi, E., Rafie, N., Miraghajani, M., Kafeshani, M. 2019. Barberry (Berberis vulgaris L.) is a safe approach for management of lipid parameters: A systematic review and meta-analysis of randomized controlled trials. Complement. Ther. Med. 43: 117-124.
- Hanachi, P., Golkho, S.H. 2009. Using HPLC to determination the composition and antioxidant activity of Berberis vulgaris. European Journal of Scientific Research. 29(1):47-54.
- Hassanpour, H., Alizadeh, S. 2016. Evaluation of phenolic compound, antioxidant activities and antioxidant enzymes of barberry genotypes in Iran. Sci Hortic. 200: 125-130.
- Larson, R.A. 1988. The antioxidants of higher plants. Phytochemistry. 27: 969-978.
- Marianne, L., Engelhart, M.D. 2002. High ıntakes of antioxidant vitamins C and E may lower the risk of alzheimer’s disease. Journal of the American Medical Association 287 (24): 3223.
- Marja, P.K., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kajula T.S., Heinonen, M. 1999. Antioksidant Activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry (47): 3954-3962.
- Meliani, N., Dib A.E.M., Allali H., Boufeldja T. 2011. Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats, Asian Pacific Jor. of Tropical Biomedicine. 1(6): 468-471.
- Moein, M., Sabahi, Z., Salim, H. 2020. A glance at Berberis integerimma pharmacological effects and its active constituents. Trends in Pharmaceutical Sciences. 6(1): 1-10.
- Moreno, M.I.N., Isla, M.I., Sampietro, A.R. and Vattueno, M.A. 2000. Comparison of the free radical scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71:109-114.
- Motalleb, G., Hanachi, P., Kua, S.H., Fauziah, O., Asmah, R. 2005. Evaluation of phenolic content and total antioxidant activity in Berberis vulgaris fruit extract. Journal of Biological Sciences. 5(5): 648-653.
- Oyaizu, M. 1986. Study on products of Browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315.
- Özkan K., Açıkgöz, Z. 2007. Kanatlı kümes hayvanlarının beslenmesi. 1.Baskı, Hasad Yayıncılık, İstanbul.
- Sarla, S., Subhash, C., Alok, K.S. 2011. Berberis asiatica future based excellent fruit in nutritional profile, antimicrobial and antioxidant ingredients. International research journal of pharmacy. 2(12): 213-216.
- Shamsa, F., Ahmadiani, A., Khosrokhavar, R. 1999. Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. Journal of Ethnopharmacology. 64, 161-166.
- Sharifi, F., Poorakbar, L. 2015. The survey of antioxidant properties of phenolic compounds in fresh and dry hybrid Barberry fruits (Berberis integerrima× vulgaris). Cumhuriyet Science Journal. 36(3): 1609-1617.
- Slinkard, K., Singleton, V.L. 1977. Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28:49-55.
- Thangapazham, R.L., Sharma, A., Maheshwari, R.K. 2006. Multiple molecular targets in cancer chemoprevention by curcumin. The AAPS Journal. 8(3): 443.
- Zargari, A. 1983. Medicinal Plants, Tehran University Press, Tehran 1, 68.
There are 29 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Project Number | SYO.A4.20.002 |
| Publication Date | October 24, 2021 |
| Submission Date | June 7, 2021 |
| Published in Issue | Year 2021 Volume: 8 Issue: 4 |
