Türkiye’nin Bingöl Bölgesinde Tanımlanan AMV (Yonca Mozaik Virüs) İzolatının Kapsid Protein Genine Dayalı Filogenetik İlişkisi

Year 2022, Volume: 9 Issue: 1, 166 - 172, 22.01.2022

Abdullah Güller , Mustafa Usta , Gülüstan Korkmaz

https://doi.org/10.30910/turkjans.947617

Cited By: 2

Abstract

Yonca mozaik virüsü (AMV), yem bitkileri arasında yonca bitkisinin en önemli viral hastalıklarından biridir ve yıllık olarak önemli ekonomik kayıplara neden olur. Etmen ayrıca domates, patates ve biber gibi diğer kültür bitkilri için de potansiyel bir öneme sahiptir. AMV'nin tanımlanması ve filogenetik ilişkileri, ters transkripsiyon-polimeraz zincir reaksiyonu (RT-PCR) ve ardından bakteriyel klonlama ile gerçekleştirildi. 12 yonca örneğinin cDNA'sı AMV'nin kapsid protein genine (CP) özgü primer çiftleri kullanılarak RT-PCR testlerine tabi tutuldu ve beklendiği gibi yaklaşık 700 bp'lik bir DNA fragmenti elde edildi. Amplikonlar doğrudan klonlandı ve daha sonra ortaya çıkan diziler genbankasına kaydedildi (Ulaşım No: MW962977 ve MW962976). Her iki sekansın BLASTn analizi, yoncadan elde edilen AMV virüsü izolatlarının, dünyadaki çeşitli bitkilerden izole edilen diğer AMV izolatlarına oldukça benzer olduğunu ve nükleotit benzerliğinin %97 ile %99.37 arasında değiştiğini gösterdi. CP gen dizilerine dayanarak elde edilen filogenetik dendrogramın sonuçları, bizim izolatlarımızın Türkiye'deki yonca bitkilerinden izole edilen dört AMV izolatı ile yakından ilişkili olduğunu açıkça ortaya koymuştur.
Bildiğimiz kadarıyla bu çalışma, Bingöl ilinde sararma, beneklenme ve yaprak anormallikleri gösteren yonca bitkilerinde belirlenen AMV virüsünün ve moleküler filogenisinin ilk raporudur.

Keywords

Yonca Mozaik Virüsü, Filogenetik Ağaç, Klonlama, Kapsid Proteini, RT-PCR

Supporting Institution

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Project Number

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Phylogenetic Relationship of Alfalfa Mosaic Virus (AMV) Isolate Identified in Bingöl Province of Turkey

Abstract

Alfalfa mosaic virus (AMV) is one of the most important viral diseases of alfalfa plant among the forage crop, causing significant annual economic losses. The agent is also of potential importance to other cultivars such as tomatoes, potatoes, and peppers in most cases. The identification and phylogenetic relationships of AMV were carried out by reverse-transcription polymerase chain reaction (RT-PCR), following by bacterial cloning. The cDNA of alfalfa samples (12) were subjected to RT-PCR tests using primer pairs, specific for the capsid protein gene (CP) of AMV, resulting in a DNA fragment of approximately 700 bp as expected. The amplicons were directly cloned and then resulting sequences were deposited in GenBank (Acc. No: MW962977, MW962976). The BLASTn analysis of both sequences demonstrated that AMV virus isolates from alfalfa were highly similar to other AMV isolates from various crops in the world, with nucleotide identity ranging from 97 to 99.37%. The results of phylogenetic dendrogram based on CP gene sequences clearly suggested that our isolates are closely related to four AMV isolates from alfalfa in Turkey.
To our knowledge, this study is the first report of molecular phylogeny and AMV presence in alfalfa exhibiting yellowing, mottling, and leaves abnormalities in Bingöl province, Turkey.

Keywords

Alfalfa mosaic virus, Phylogenetic tree, Cloning, Capsid Protein, RT-PCR

Project Number

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References

• Abdalla, O.A., Al-Shahwan, I.M., AL-Saleh, M.A. and Amer M,A, 2019. Molecular characterization of Alfalfa mosaic virus (AMV) isolates in alfalfa and other plant species in different regions in Saudi Arabia. European Journal of Plant Pathology, 156: 603–613.
• Al-Saleh, M.A. and Amer, M.A. 2013. Biological and Molecular variability of Alfalfa mosaic virus affecting alfalfa crop in riyadh region. The Plant Pathology Journal, 29(4) : 410-417.
• Al-Shahwan, I.M., Abdalla, O.A., Al-Saleh, M.A. and Amer, M.A. 2017. Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi Journal of Biological Sciences, 24 (6): 1336- 1343.
• Brunt, A., Crabtree, K., and Gibbs, A. 1990. Viruses of Tropical Plants. CAB International, London. Chalam, V.C., Parakh, D.B., Khetarpal, R.K., Maurya, A.K., Anju Jain and Shamsher Singh, 2008. Interception of seed-transmitted viruses in cowpea and mungbean germplasm imported during 2003. Indian Journal of Virology, 19(1): 12-16.
• Colimba, J., Falcón, E., Castro, E.R., Davila-Aldas, D., Pallas, V., Sanchez-Navarro, J.A., and Gomez, G. 2016. First report of Alfalfa mosaic virus in red pepper plants in Ecuador. Plant Disease, 100(5): 1026.
• Çalı, S. 1990. Orta Anadolu Bölgesi Yonca Ekim Alanlarında Görülen Virüs Enfeksiyonlarının Saptanması, Yabancı Otlar ve Tohumla Taşınması Üzerine Araştırmalar. Adana Zirai Mücadele Araştırma Ens. Müdürlüğü, Araştırma Yayınlan Serisi, Yayın No: 72.
• Çetinkıran, A.D. and Baloğlu, S. 2011. Detection of the Alfalfa mosaic virus in pepper fields in Adana and Mersin. Ç.Ü Fen ve Mühendislik Bilimleri Dergisi, 26 (2): 89- 98.
• Çıtır, A. 1982. Erzurum ve çevresinde tohumluk patateslerdeki virüs hastalıkları ve bunlarm tanılanması üzerinde bazı çalışmalar. Doğa Bilim Dergisi, 6(3): 99-109.
• Çulal Kılıç, H. and Yardımcı, N. 2015. Occurrence of Alfalfa mosaic virus (AMV) infecting bean crop in Burdur Province, Turkey. Asian Journal of Agriculture and Food Sciences, 3(2): 173- 177.
• Edwardson, J.R. and Christie, R.G. 1986. Viruses infecting forage legumes. Vol. I. Fla. Agric. Exp. Stn. Monogr. 14: 42.
• Erdiller, G. and Lesemann, D.E. 1985. Türkiye İçin Yeni Bir Virüs Hastalığı "Alfalfa Mosaic Virus". Ankara Üniv. Ziraat Fakültesi Yıllığı, 35: 20-25.
• Fidan H., Adak N.A., Konuksal A., Akerzurumlu E. and Yılmaz M.A. 2012. Occurrence of Alfalfa mosaic virus (AMV) diseases on potato crops in Northern Cyprus. Acta Horticulturae, 960: 341-346.
• Fidan, H. and Sarıkaya, P. 2020. Virus diseases in eggplant (Solanum melongena) cultivation in Antalya province. Mediterranean Agricultural Sciences, 33(1): 27-35.
• Finetti Sialer, M.M., Parrella, G., Papanice, M., Vovlas, C. and Gallitelli, D. 2000. Biodiversity of viruses infecting tomato in Italy: methods for diagnosis and diversification. Bulletin OEPP, 30(2): 301-304.
• Fisher, J.R. and Nameth, S.T. 2000. Virus assessment of Ajuga reptans cultivars reveals alfalfa mosaic, tobacco streak, and cucumber mosaic (CMV) viruses, and a CMV satellite RNA. Horticultural Science, 35(2): 230-234.
• Fletcher, J.D. 2001. New hosts of Alfalfa mosaic virus, Cucumber mosaic virus, Potato virüs Y, Soybean dwarf virus, and Tomato spotted wilt virus in New Zealand. New Zealand Journal of Crop and Horticultural Science, 29 (3): 213-217.
• Hajimorad, M.R. and Francki, R.I.B. 1988. Alfalfa mosaic virus isolates from lucerne in South Australia: Biological variability and antigenic similarity. The Annals of Applied Biology, 113: 45–54.
• Hulse, L.B., Helms, K. and Waterhouse, P.M. 1993. National survey of viruses in pastures of subterranean clover. III. Equipment for large scale screening of viruses by ELISA. Australian Journal of Agricultural Research, 44(8): 1883-1890.
• Mangeli, F., Massumi, H., Alipour, F., Maddahian, M., Heydarnejad, J., Hosseinipour, A., Amid-Motlagh, M.H., Azizizadeh, M. and Varsani, A. 2019. Molecular and partial biological characterization of the coat protein sequences of Iranian Alfalfa mosaic virus isolates. Journal of Plant Pathology, 101: 73-742.
• Martínez-Priego, L., Córdoba, M.C. and Jordá, C. 2004. First report of Alfalfa mosaic virus in Lavandula officinalis. Plant Disease, 88(8): 908.
• Massumi, H., Maddahian, M., Heydarnejad, J., Hosseini Pour, A. and Farahmand, A. 2012. Incidence of Viruses Infecting Alfalfa in the Southeast and Central Regions of Iran. Journal of Agricultural Science and Technology, 14: 1141-1148.
• Mohammed, A.A.S. and Mahmoud, A.A. 2013. Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region. The Plant Pathology Journal, 29(4): 410-417.
• Moreira, A. and Fageria, N.K. 2010. Liming influence on soil chemical properties, nutritional status and yield of alfalfa grown in acid soil. The Revista Brasileira de Ciência do Solo, 34: 1231-1239.
• Ozdemir, S., Erilmez, S. and Paylan, I.C. 2011. First report of Alfalfa mosaic virus in eggplant in Turkey. The Journal of Plant Pathology, 93(4): 63-89.
• Özdemir, S. and Erilmez, S. 2007. Denizli ilinde yetiştirilen biber, patlıcan ve marul üretim alanlarında bazı viral etmenlerin saptanması. Türkiye II. Bitki Koruma Kongresi Bildirileri.27-29 Ağustos Isparta. 114s.
• Parrella, G., Cavicchi, L. and Bellardi, M.G. 2012. First Record of Alfalfa mosaic virus in Teucrium fruticans in Italy. Disease Notes, 96(2): 294.
• Parrella, G., Acanfora, N. and Bellardi, M.G. 2010. First record and complete nucleotide sequence of Alfalfa mosaic virus from Lavandula stoechas in Italy. Plant Disease, 94(7): 924.
• Peña, E., Olate, E., Chorbadjian, R.A. and Rosales, I.M. 2011. First Report of Alfalfa mosaic virus Infection in Viburnum tinus in Chile. Plant Disease, 95(9): 1198.
• Pourrahim, R. and Farzadfar, S. 2016. Biological and Molecular Characterization of Alfalfa Mosaic Virus Infecting Trumpet Creeper (Campsis radicans) in Iran. Journal of Phytopathology, 164: 276–280.
• Ragsdale, D.W., Radcliffe, E.B. and DiFonzo, C.D. 2001. Epidemiology and feld control of PVY and PLRV. In: Virus and Virus-like Diseases of Potatoes and Production of Seed-potatoes. Eds. Loebenstein G., PH Berger, AA Brunt and RH Lawson. Huwer Academic Publishers, Dordrecht: 237-270.
• Rubies-Autonell, C. and Turina, M. 1994. Alfalfa mosaic virus (AMV) isolated from kenaf (Hibiscus cannabinus). Phytopathologia Mediterranea, 33 (3): 234-239.
• Sertkaya, G., Çarpar, H. and Sertkaya, E. 2017. Detection of Alfalfa mosaic virus (AMV) in Potato Production Areas in Hatay Province of Turkey. Journal of the Institute of Science and Technology, 7(1): 23-29.
• Shepherd, R.J., Hall, D.H. and Purcifull, D.E. 1964. Occurrence of the Alfalfa Mosaic Virus in Sugar Beet in California. Journal of A. S. S: B. T. 13: 4.
• Sofy, A.R., Sofy, M.R., Hmed, A.A., Dawoud, R.A., Refaey, E.E., Mohamed, H.I. and El-Dougdoug, N.K. 2021. Molecular Characterization of the Alfalfa mosaic virus Infecting Solanum melongena in Egypt and the Control of Its Deleterious Effects with Melatonin and Salicylic Acid. Plants, 10: 459.
• Stanković, I., Vrandečić, K., Ćosić, J., Milojević, K. Bulajić, A. and Krstić, B. 2014. The spreading of Alfalfa mosaic virus in lavandin in Croatia. Pestic. Pesticides and Phytomedicine, 29(2): 115–122.
• Topkaya, Ş. 2020. Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province. Journal of Agricultural Faculty of Gaziosmanpasa University, 37 (1): 53-59.
• TUİK, 2020. Türkiye İstatistik Kurumu, Bitkisel Üretim İstatistikleri. www.tuik.gov.tr Erişim tarihi: 18.03.2020.
• Usta, M. and Güller, A. 2020. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Journal of the Institute of Science and Technology, 10(4): 2366-2377.
• Xu, H. and Nie, J. 2006. Identification, characterization and molecular detection of Alfalfa mosaic virus in potato. Phytopathology, 96: 1237−1242.
• Yardımcı, N. and Açıkgöz, S. 1997. Erzurum Yöresi Yoncalarında, Mozayik Hastalığı Etmeninin Test Bitkileri İle Tanılanması. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 28(2): 195-205.
• Yardımcı, N., Eryiğit H. and Erdal, İ. 2007. Effect of Alfalfa Mosaic Virus (AMV) on The Content of Some Macro- And Micronutrients in Alfalfa. Journal of Culture Collections, 5: 90-93.
• Zadjali, A.D., Matrooshi, A.R. and Moghal, S.M. 2002. Occurrence, distribution and properties of Alfalfa Mosaic Virus. Sultan Qaboos University Journal for Scientific Research - Agricultural Sciences, 7(1):47-51.
• Zitikaitė, I. and Samuitienė, M. 2008. Identification and some properties of Alfalfa mosaic alfamovirus isolated from naturally infected tomato cropbiologija. Biologija, 54 (2): 83–88.